期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 8, 期 5, 页码 2259-2269出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2008.501
关键词
carbon nanotubes; poly(ethylene glycol); fusogenic polymer; T-lymphocytes; endocytosis; flow cytometry
类别
资金
- NCI NIH HHS [U54 CA119335-02] Funding Source: Medline
In this paper we report the fabrication of a multivalent, cell-type specific and cytoplasmic delivery system based on single-walled carbon nanotubes. The latter were functionalized through adsorption of phospholipids terminated by biotinylated PEG chains functionalized with fluorochrome-coupled neutravidin, and subsequently with antibodies (anti-CD3 epsilon and anti-CD28) for T cell receptor post-signaling endocytosis and a synthetic fusogenic polymer for disruption of lysosomal compartments. The biomimetic nanoassemblies were composed by PEGylated individual/very small bundles of carbon nanotubes having an average length and a standard deviation of 176 nm and 77 nm, respectively. The nanoassemblies were stably dispersed under physiological conditions, visible by conventional optical and confocal microscopy and specifically targeted to T cells both in vitro and in living animals. The addition of a fusogenic polymer to the nanoassemblies did not affect the cellular uptake and allowed the release into the cytosol of the targeted cells both in vitro and in the animals. The present manuscript is the first report about the cytoplasmic delivery of carbon nanotubes in a specific cell type in intact animals and paves the way for their use as in vivo intracellular delivery systems.
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