期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 13, 期 9, 页码 4097-4108出版社
SPRINGER
DOI: 10.1007/s11051-011-0353-8
关键词
Mesoporous silica; MCM-41; Transfection; Gene delivery; PEI; Nanoparticles; Nanomedicine
资金
- Ministerio de Ciencia e Innovacion [MAT2009-14695-C04-02]
- Fundacion Mutua Madrilena
- Fundacion Ramon Areces
- Comunidad de Madrid
- 2006 Ramon y Cajal program [order ECI/158/2005]
- Fondo de Investigaciones Sanitarias
Mesoporous silica nanoparticles have been synthesized and functionalized with four different types of molecules containing amino groups, i.e., with primary amines only, with quaternary amines, with quaternized cyclic amines, or with polyethylenimine (PEI), which is formed by primary, secondary, and tertiary amines. These nanoparticles were then incubated with reporter plasmids and the ability of the resulting complexes to transfect human cells was studied. Only nanoparticles functionalized with PEI were efficient for transfection. The agglomeration behavior and the electrokinetic potential of the nanoparticle-plasmid complexes have been studied, as well as their cell internalization behavior using a fluorescent-labeled plasmid that allows its monitorization by confocal microscopy. The results indicate that the efficiency of PEI-functionalized nanoparticles for transfection resides to some extent in the different characteristics imparted to the nanoparticles regarding agglomeration and surface charge behavior.
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