Article
Genetics & Heredity
Qi Zeng, Beijuan Hu, Andres Hortas Blanco, Wanchang Zhang, Daxian Zhao, Paulino Martinez, Yijiang Hong
Summary: This study combined Illumina RNA-seq and PacBio Isoform Sequencing to analyze the gonads of H. schlegelii and identified genes related to sex determination and gonadal development.
FRONTIERS IN GENETICS
(2022)
Article
Fisheries
Xuenan Li, Shangle Feng, Xingrong Xuan, He Wang, Xiaoya Shen, Yige Chen, Yuanshuai Fu, Zhiyi Bai, Wenjuan Li
Summary: In this study, the differentially expressed proteins (DEPs) of the mantle and pearl sac in the production of freshwater pearls were identified and the related signaling pathways of biomineralization and immune response were analyzed. The expression levels of selected proteins were verified using quantitative real-time PCR. The findings contribute to the understanding of pearl formation and immune response mechanisms.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Physiology
Yayu Wang, Xiaoqiang Wang, Jingyuan Ge, Guiling Wang, Jiale Li
Summary: The regulatory element Tra-2 plays a critical role in sex formation in insects, with Hctra-2 identified in Hyriopsis cumingii showing potential involvement in regulating gonadal development. The expression of Hctra-2 is highest in the testes compared to ovaries, indicating a potential role in sex determination mechanisms in shellfish.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Pattanan Nuchan, Uthaiwan Kovitvadhi, Akkarasiri Sangsawang, Satit Kovitvadhi, Pakasinee Klaimala, Nopparat Srakaew
Summary: This study evaluated the biochemical and cellular responses of the freshwater mussel, Hyriopsis bialata, to the herbicide atrazine. The results showed that the mussels were resistant to atrazine and did not show significant changes in detoxification enzymes. However, DNA damage was observed in all tissues of the treated mussels. This suggests that H. bialata could be a potential candidate for monitoring aquatic toxicity using DNA damage as a biomarker.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
ShangLe Feng, He Wang, XueNan Li, WenJuan Li, ZhiYi Bai
Summary: This study found that the Hc-CCND2 gene is widely expressed in freshwater pearl mussel and plays an important role in the cell cycle. Overexpression of Hc-CCND2 promotes cell proliferation, which is significant for improving the proliferation rate of shellfish cells in in vitro cultures.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Fisheries
He Wang, Kai Huang, Shangle Feng, Xuenan Li, Luping Wang, Xingrong Xuan, Yuanshuai Fu, Zhiyi Bai, Wenjuan Li
Summary: This study investigated the most suitable position for inserting nucleuses in the visceral mass of freshwater pearl mussels by analyzing physiological responses and cultured pearl biology. It was found that the axe foot visceral group was the most appropriate position. The insertion of nucleuses caused metabolic disorders and immune inflammation. Blood cells showed an early immune defense function and the synthesis of granulosa in the pearl capsule contributed to the formation of thicker pearl deposits. This study filled the knowledge gaps in cultivating pearls in the visceral mass and provided a theoretical basis for artificial pearl breeding.
AQUACULTURE RESEARCH
(2022)
Article
Biology
Xuenan Li, Shangle Feng, He Wang, Xiaoya Shen, Yige Chen, Xingrong Xuan, Yuanshuai Fu, Zhiyi Bai, Wenjuan Li
Summary: In this study, the function of IGF1 in Hyriopsis cumingii was explored. It was found that IGF1 plays a role in promoting cell proliferation through the PI3K/AKT signaling pathway. The results provide a new method to solve the low proliferative capacity of mantle cells in vitro and contribute to a better understanding of the growth regulation mechanism of H. cumingii.
Article
Biochemistry & Molecular Biology
Zongyu Liu, Xin Jin, Yulin Miao, Ping Wang, Yang Gu, Xiaozhao Shangguan, Lijing Chen, Guiling Wang
Summary: C-Mos, a proto-oncogene, regulates oocyte maturation by activating the classical MAPK pathway in cells. This study identified C-Mos in Hyriopsis cumingii and found that it is highly expressed during early gonad development, indicating its involvement in gonadal development in this species. Knockdown of C-Mos resulted in down-regulation of ERK and P90rsk.
Article
Fisheries
Yayu Wang, Ya Chen, Mulian Cao, Xiaoqiang Wang, Guiling Wang, Jiale Li
Summary: Wnt2 plays a key role in immune regulation and gonadal development in Hyriopsis cumingii, with high expression in the ovary and gill. MiR-1988b-5p negatively regulates Wnt2 to affect the expression of other key genes in the Wnt signaling pathway.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Jinpan Zhang, Baiying Guo, Mengying Zhang, Jiale Li, Zhiyi Bai
Summary: The gene HcStAR-like is crucial for both the accumulation of carotenoids and inner shell pigmentation in the freshwater pearl mussel, Hyriopsis cumingii. Expression levels of HcStAR-like also correlate with shell color in different tissues of the mussel.
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
(2022)
Article
Fisheries
Honghui Hu, Chaohu Sun, Zhiyi Bai, Jiale Li
Summary: This study estimated the heritabilities and genotype by environment interactions on inner shell color and growth traits of Hyriopsis cumingii reared in different water depths and mud substrates. The results showed that under the same mud substrate condition, animals reared in shallower water depths exhibited better performance in shell color and growth, while animals reared with mussel pond mud substrate showed superior performance in shell color and growth traits under the same water depth condition.
Article
Multidisciplinary Sciences
Juergen Geist, Helmut Bayerl, Bernhard C. Stoeckle, Ralph Kuehn
Summary: Ensuring genetic integrity is crucial in conservation-oriented captive breeding programs, especially for endangered species like freshwater pearl mussels. A study comparing the genetic makeup of captive-bred mussels from different facilities with their original source populations found close resemblance but also signs of inbreeding. Long-term breeding over multiple years can minimize erosion of genetic diversity, while effective breeding strategies and collaboration networks can enhance resilience and effectiveness in conservation efforts.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Xuming Xu, Yaru Xu, Nan Xu, Baozhu Pan, Fengyue Shu, Jinren Ni
Summary: Thirty-five PPCPs were measured in freshwater pearl mussels in Poyang Lake, China, and their response to sedimentary PPCPs were observed. The concentration of PPCPs in soft tissues of mussels was much higher than that in sediments, with anti-inflammatories being the primary contaminants detected. The gonads of mussels were identified as a hotspot for these contaminants, and correlation analysis showed that they were more likely to assimilate triclosan from sediments. These findings highlight the concern on the potential effects of sedimentary PPCPs and the necessity to formulate control strategies for a healthy lake.
MARINE POLLUTION BULLETIN
(2023)
Article
Immunology
Zhiyi Bai, He Wang, Xuenan Li, Xiaoya Shen, Yige Chen, Yuanshuai Fu, Wenjuan Li
Summary: This study investigates the biological characteristics of fetal bovine serum (FBS) and Hyriopsis cumingii serum (HCS), and compares their effects on mussel cell culture. The results show significant differences in total protein, calcium ions, and osmotic pressure between the two serums, and the HCS culture system promotes better activity of mantle cells compared to the FBS culture system. Furthermore, HCS contains a high abundance of immune factors that play important roles in mussel cell culture.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Huan-huan Yang, Chan Zhong, Le-chang Sun, Ya-ke Li, Hu Chen, Guo-ping Wu
Summary: Substituting NaCl with KCl, MgCl2, and CaCl2 in low levels can improve the functional properties of myofibrillar protein, especially in terms of solubility and ordered microstructure.