Article
Multidisciplinary Sciences
Yuanyuan Chen, Reka Toth, Sara Chocarro, Dieter Weichenhan, Joschka Hey, Pavlo Lutsik, Stefan Sawall, Georgios T. Stathopoulos, Christoph Plass, Rocio Sotillo
Summary: This study demonstrates that Club cells utilize regeneration mechanisms to develop lung adenocarcinoma (LUAD). Club cells undergo an epigenetic switch and acquire an AT2-like phenotype during tumor formation. This research highlights the significant role of Club cells in the initiation of LUAD.
NATURE COMMUNICATIONS
(2022)
Review
Physiology
Jennifer M. K. Nguyen, Douglas N. Robinson, Venkataramana K. Sidhaye
Summary: COPD is a respiratory disease characterized by breathing difficulties, with current treatments focusing on symptom relief rather than cure. Emerging research on upstream pathways such as mitochondrial function, cytoskeletal structure, and airway hydration may provide breakthroughs in COPD therapeutics.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Rick Heida, Paul Hagedoorn, Melle C. van Meel, Jurrie E. R. Prins, Frederike S. Simonis, Renate Akkerman, Anke L. W. Huckriede, Henderik W. Frijlink, Anne H. de Boer, Wouter L. J. Hinrichs
Summary: A challenge in developing dry powder formulations for inhalation is the poor reproducibility of administration to small laboratory animals. Current devices for delivering dry powder formulations to small rodents often fail to function optimally due to the use of the same puff of air for both powder dispersion and aerosol delivery. A novel aerosol generator was introduced, allowing the administration of a pre-generated aerosol to mice lungs, with controlled powder delivery. Although the emitted aerosol showed favorable size range for lung deposition and reproducibility, in vivo fluorescent imaging revealed significant differences in dose deposition and powder distribution among mice, suggesting inevitable biological variation in lung deposition.
Review
Pediatrics
Venkatakrishna Kakkilaya, Kanekal Suresh Gautham
Summary: The harmful effects of mechanical ventilation on preterm lung have been established, making less invasive surfactant administration (LISA) a desirable alternative. While LISA has been widely adopted in other countries, its use is not widespread in the United States.
PEDIATRIC RESEARCH
(2023)
Article
Engineering, Biomedical
Olive Jung, Yen-Ting Tung, Esther Sim, Yu-Chi Chen, Emily Lee, Marc Ferrer, Min Jae Song
Summary: The COVID-19 pandemic has emphasized the importance of human respiratory tract-based assay platforms for the development of antivirals and disease-modifying therapies. This study has developed vascularized lung tissue models for the alveoli and the small airway, which can be used for the investigation of respiratory viral infections and drug discovery.
Article
Cell Biology
Frida Berlin, Sofia Mogren, Camilla Ly, Sangeetha Ramu, Morten Hvidtfeldt, Lena Uller, Celeste Porsbjerg, Cecilia K. Andersson
Summary: Bronchial and alveolar remodeling and impaired epithelial function are common in chronic respiratory diseases. This study found that tryptase released by mast cells enhanced the growth of bronchial and alveolar epithelial cells and shortened cell division intervals. Tryptase also increased the expression of anti-apoptotic protein BIRC3 and growth factor release. These findings suggest that intraepithelial and alveolar release of tryptase by mast cells may contribute to the disturbance of bronchial and alveolar homeostasis.
Review
Health Care Sciences & Services
Lei Fang, Michael Roth
Summary: Airway wall remodeling is a pathology seen in chronic inflammatory lung diseases, with challenges including lack of consensus in defining it, methodologic limitations, absence of anti-remodeling therapies, and difficulty in determining study endpoints and outcomes.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Narumon Changsan, Chutima Sinsuebpol
Summary: Chitosan nanoparticles co-loaded with isoniazid and pyrazinamide were prepared using the ionic gelation method. The impact of different TPP to chitosan mass ratios on nanoparticle generation was studied. The selected nanoparticle formulations exhibited good aerosol performance and safety for potential use in tuberculosis treatment in the respiratory tract.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Kim Jee Goh, Ee Kim Tan, Hao Lu, Sudipto Roy, N. Ray Dunn
Summary: New hPSC line with dual fluorescent reporters, NKX2-1(GFP);TP63(tdTomato), has been developed to specifically label and track human basal cells, facilitating further research on their origins and roles in lung development.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Chandani Sen, Delilah Freund, Brigitte N. Gomperts
Summary: Respiratory diseases are a leading cause of death globally, but the development of therapies has been slow due to a lack of relevant lung models. Three-dimensional co-culture systems, known as organoids/spheroids, have emerged as a promising tool to bridge the gap between in-dish and in-tissue cell behavior. These models have diverse applications in understanding lung biology, disease modeling, and drug screening.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Medicine, General & Internal
Eunsu Kang, Byeong Cheol Lee, Jae Hong Park, Sang Eun Lee, Se Hun Kim, Daeseok Oh, Dae Yun Choi, Myoung Jin Ko
Summary: The coordination of consciousness and muscular function recovery is crucial during emergence from anesthesia. Timing of sugammadex administration does not significantly affect upper airway obstruction, but a higher minimum alveolar concentration (MAC) is associated with an increased risk of upper airway obstruction after tracheal extubation.
MEDICINA-LITHUANIA
(2021)
Article
Engineering, Chemical
Estefania Fernandez-Paz, Cristina Fernandez-Paz, Sheila Barrios-Esteban, Irene Santalices, Noemi Csaba, Carmen Remunan-Lopez
Summary: This work presents a novel micro-nanoplatform for pulmonary administration of active ingredients. The chitosan-based nanocapsules with or without hyaluronic acid were encapsulated in mannitol microspheres using a simple spray-drying technique, resulting in dry powders suitable for inhalation. In vitro studies showed high cell viabilities and internalization rates of the nanocapsules.
Article
Pharmacology & Pharmacy
Mahwash Mukhtar, Noemi Csaba, Sandra Robla, Ruben Varela-Calvino, Attila Nagy, Katalin Burian, David Kokai, Rita Ambrus
Summary: A nano-DPI system was developed by combining isoniazid (INH) with mannosylated chitosan (MC) and hyaluronic acid (HA), and preparing dry powder through an ionic gelation method. The nanoparticles showed no significant toxicity to cells, good compatibility with red blood cells, and activated T-cell response. Moreover, the nanoparticles were translocated in macrophages.
Article
Food Science & Technology
Hyo Jin Kim, Jiwon Yang, Kalahe Hewage Iresha Nadeeka Madushani Herath, You-Jin Jeon, Young-Ok Son, Doyoung Kwon, Hyun Jung Kim, Youngheun Jee
Summary: The study finds that prolonged oxidative stress and lipid peroxidation are induced by particulate matter (PM) exposure in alveolar macrophages (AMs) and alveolar epithelial cells. Specifically, PM exposure leads to enhanced oxidative DNA damage in M2-polarized AMs. However, Sargassum horneri ethanol extract (SHE) repairs oxidative DNA damage in both M1- and M2-polarized AMs and reduces polarization imbalance caused by PM exposure.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Cell Biology
Arvind Konkimalla, Aleksandra Tata, Purushothama Rao Tata
Summary: This article describes the existence of lung epithelial cells and their lineage relationships during normal state, discusses the cellular sources contributing to repair, regeneration, and remodeling following different injury models, and also explores the role of regeneration-associated cell states in disease pathogenesis.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2022)
Article
Allergy
T. Otani, H. Iwamoto, Y. Horimasu, K. Yamaguchi, S. Sakamoto, T. Masuda, S. Miyamoto, T. Nakashima, K. Fujitaka, H. Hamada, N. Hattori
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Respiratory System
Hiroki Tanahashi, Kakuhiro Yamaguchi, Koji Kurose, Satoshi Nakao, Shinjiro Sakamoto, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Taku Nakashima, Hiroshi Iwamoto, Kazunori Fujitaka, Hironobu Hamada, Toru Oga, Mikio Oka, Noboru Hattori
Summary: This study explored the potential of HMGB1 as a blood marker to predict the development and severity of checkpoint inhibitor pneumonitis (CIP) caused by anti-PD-1/PD-L1 therapy in non-small cell lung cancer (NSCLC) patients.
Article
Oncology
Angela D. Trobaugh-Lotrario, Rudolf Maibach, Daniel C. Aronson, Arun Rangaswami, Beate Haeberle, Allison F. O'Neill, Irene Schmid, Marc Ansari, Tomoro Hishiki, Sarangarajan Ranganathan, Rita Alaggio, Ronald R. de Krijger, Yukichi Tanaka, Soo-Jin Cho, Christian Vokuhl, Rebecca Maxwell, Mark Krailo, Eiso Hiyama, Piotr Czauderna, Milton Finegold, James H. Feusner, Marcio H. Malogolowkin, Rebecka L. Meyers, Dolores Lopez-Terrada
Summary: Hepatoblastoma is the most common malignant liver tumor in children, and small cell undifferentiated histology and low alpha-fetoprotein levels have been associated with poor prognosis. It is important to differentiate rhabdoid tumors from hepatoblastoma with small cell undifferentiated component or low alpha-fetoprotein levels, as the treatment differs significantly. When rhabdoid tumors are correctly diagnosed, neither the presence of small cell undifferentiated component nor low alpha-fetoprotein levels are associated with poor prognosis.
Article
Biology
Sungmin Yang, Kyungsik Ha, Woojeung Song, Masashi Fujita, Kirsten Kuebler, Paz Polak, Eiso Hiyama, Hidewaki Nakagawa, Hong-Gee Kim, Hwajin Lee
Summary: We have developed COOBoostR, a computational method that can predict the tissue or cell of origin for various types of cancer. COOBoostR uses regional somatic mutation density information and chromatin mark features in an extreme gradient boosting-based machine-learning algorithm. By ranking chromatin marks from different tissue and cell types, COOBoostR can determine the most likely tissue or cell of origin based on the somatic mutation density landscape of a sample.
Article
Infectious Diseases
Keitaro Omori, Hiroki Kitagawa, Kakuhiro Yamaguchi, Shinjiro Sakamoto, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Taku Nakashima, Hiroshi Iwamoto, Kazunori Fujitaka, Hironobu Hamada, Kayoko Tadera, Toshihito Nomura, Norifumi Shigemoto, Gaku Aoki, Noboru Hattori, Hiroki Ohge
Summary: The prevalence of nontuberculous mycobacteria (NTM) infections is increasing worldwide. However, there is limited research on the clinical characteristics of extrapulmonary NTM. In this study, researchers analyzed the species distribution, infected sites, and risk factors of extrapulmonary NTM compared to pulmonary NTM. The results showed that younger age, corticosteroid use, and AIDS were associated with extrapulmonary NTM, and rapid-growing mycobacteria (RGM) were more prevalent in extrapulmonary NTM. The findings provide a better understanding of extrapulmonary NTM.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Taku Nakashima, Keitaro Omori, Masashi Namba, Kakuhiro Yamaguchi, Shinjiro Sakamoto, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Hiroshi Iwamoto, Kazunori Fujitaka, Hironobu Hamada, Noboru Hattori
Summary: This study investigates the potential of soluble B7H3 (sB7H3) as a biomarker to predict prognosis in patients with fibrosing interstitial lung diseases (ILDs). The results show that BALF sB7H3 levels at the time of ILD diagnosis have independent prognostic potential on 5-year survival in patients with fibrosing ILDs.
RESPIRATORY MEDICINE
(2023)
Article
Multidisciplinary Sciences
Shinichiro Miura, Hiroshi Iwamoto, Keitaro Omori, Kakuhiro Yamaguchi, Shinjiro Sakamoto, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Taku Nakashima, Kazunori Fujitaka, Hironobu Hamada, Akihito Yokoyama, Noboru Hattori
Summary: This study aimed to clarify the clinical features of restrictive and non-restrictive preserved ratio impaired spirometry (PRISm). The results suggest that non-restrictive PRISm may be a precursor of chronic obstructive pulmonary disease and requires increased monitoring.
SCIENTIFIC REPORTS
(2023)
Article
Allergy
Hiroshi Iwamoto, Tsunahiko Hirano, Yoshihiro Amano, Keita Murakawa, Ayumi Fukatsu-Chikumoto, Yoshikazu Yamaji, Mayuka Yamane, Kazuki Anabuki, Toshihito Otani, Naoko Higaki, Shintaro Miyamoto, Takeshi Isobe, Akihito Yokoyama, Kazuto Matsunaga, Noboru Hattori
Summary: This study aimed to investigate the risk factors and clinical phenotypes associated with reduced physical activity in a wide range of patients with asthma. The study found that higher eosinophils and body mass index (BMI) were associated with reduced physical activity in patients with asthma without chronic obstructive pulmonary disease (COPD). Cluster analysis revealed four asthma phenotypes, one of which had preserved physical activity.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Oncology
Kentaro Tokumo, Takeshi Masuda, Taku Nakashima, Masashi Namba, Kakuhiro Yamaguchi, Shinjiro Sakamoto, Yasushi Horimasu, Shintaro Miyamoto, Hiroshi Iwamoto, Kazunori Fujitaka, Yoshihiro Miyata, Morihito Okada, Hironobu Hamada, Noboru Hattori
Summary: This study shows that PAI-1 is involved in tolerance to osimertinib through epithelial-mesenchymal transition in drug-tolerant non-small-cell lung cancer cells. Additionally, PAI-1 inhibitors can suppress the proliferation and expression of mesenchymal genes in tolerant cells. Based on these observations, PAI-1 may be a potential therapeutic target for overcoming osimertinib tolerance.
Article
Oncology
Takahiro Yamada, Taku Nakashima, Takeshi Masuda, Shinjiro Sakamoto, Kakuhiro Yamaguchi, Yasushi Horimasu, Shintaro Miyamoto, Hiroshi Iwamoto, Kazunori Fujitaka, Hironobu Hamada, Nobuhiko Kamada, Noboru Hattori
Summary: Increasing evidence suggests a link between intestinal microbiota, including fungi, and diseases in distal organs. This study aimed to investigate the impact of changes in intestinal fungal microbiota on the development of pulmonary fibrosis. Mice with Candida albicans overgrowth in the intestine, induced by oral administration of antibiotics and C. albicans, exhibited accelerated bleomycin-induced pulmonary fibrosis. These mice also showed enhanced Th17-type immune response, which was alleviated by IL-17A-neutralizing antibody treatment. The study highlights the involvement of IL-17A-mediated endothelial-mesenchymal transition in Candida albicans-exacerbated pulmonary fibrosis.
JOURNAL OF PATHOLOGY
(2023)
Article
Medicine, General & Internal
Takashi Oda, Hiroshi Iwamoto, Sachio Takeno, Tomohiro Kawasumi, Kota Takemoto, Manabu Nishida, Nobuyuki Chikuie, Yuichiro Horibe, Kakuhiro Yamaguchi, Shinjiro Sakamoto, Naoko Higaki, Takayuki Taruya, Yasushi Horimasu, Takeshi Masuda, Takao Hamamoto, Taku Nakashima, Takashi Ishino, Tsutomu Ueda, Kazunori Fujitaka, Hironobu Hamada, Noboru Hattori
Summary: This study aimed to investigate the etiology of olfactory dysfunction in patients with asthma and the relationship between fractional exhaled nitric oxide (FeNO) levels. The results showed that chronic rhinosinusitis was the most common etiology of olfactory dysfunction in asthma patients, with eosinophilic chronic rhinosinusitis being associated with high FeNO levels and non-eosinophilic chronic rhinosinusitis being associated with low FeNO levels. Additionally, this study supported the link between upper and lower airway inflammation in asthma patients with olfactory dysfunction.
MEDICINA-LITHUANIA
(2023)