Article
Chemistry, Analytical
Yawen Liu, Xing Huang, Han Zhu, Yongkai Lu, Hongzhi Song, Junfeng Niu, Hongxia Chen
Summary: In this paper, a biomimetic probe was synthesized by functionalizing cancer cell membrane with gold nanoparticles, achieving highly sensitive detection of circulating exosomes. This strategy offers new possibilities for clinical non-invasive cancer diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Peng He, Shannon Faris, Reddy Sudheer Sagabala, Payel Datta, Zihan Xu, Brian Callahan, Chunyu Wang, Benoit Boivin, Fuming Zhang, Robert J. Linhardt
Summary: Cholesterol, an important lipid in animal cell membranes, can bind to various soluble proteins, transport proteins, and membrane-bound proteins. However, studying the interactions between cholesterol and proteins in aqueous solutions is challenging due to the low solubility of cholesterol, often necessitating the use of organic co-solvents or surfactant additives. In this study, a biotinylated cholesterol derivative was synthesized and immobilized on a streptavidin chip for analysis using surface plasmon resonance (SPR). The kinetics of cholesterol interactions with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B, were determined.
Article
Multidisciplinary Sciences
Long Dao, Qingnan Zhao, Jiemiao Hu, Xueqing Xia, Qing Yang, Shulin Li
Summary: Currently available techniques cannot directly visualize receptor-ligand interactions and there is no publicly available method for analyzing these interactions at the single-cell level. This study describes a new microfluidic chip-based technique that allows for analysis and visualization of these interactions at the single-cell level, and demonstrates its efficacy and versatility.
Article
Chemistry, Multidisciplinary
Axel Gomes, Jose M. Carnerero, Aila Jimenez-Ruiz, Elia Grueso, Rosa M. Giraldez-Perez, Rafael Prado-Gotor
Summary: This study investigated the adsorption of lysozyme onto gold nanoparticles and obtained the free energy of binding of the system for the first time. The interactions between lysozyme and AuNPs induce aggregation of citrate-stabilized AuNPs at low concentrations. The study also found that 10 nm AuNPs were the most appropriate for this interaction.
Article
Biochemistry & Molecular Biology
Shoko Mori, Kaoru Nomura, Kohki Fujikawa, Tsukiho Osawa, Masafumi Shionyu, Takao Yoda, Tsuyoshi Shirai, Shugo Tsuda, Kumiko Yoshizawa-Kumagaye, Shun Masuda, Hideki Nishio, Taku Yoshiya, Sonomi Suzuki, Maki Muramoto, Ken-ichi Nishiyama, Keiko Shimamoto
Summary: Inducing the integration of newly synthesized proteins into appropriate locations is a crucial biological function. MPIase, a proteinaceous factor found in organisms, plays an essential role in membrane protein integration. This study investigated the interaction between MPIase and a model substrate, revealing the detailed mechanisms of membrane protein integration. The results demonstrated direct interactions between MPIase and the substrate protein, shedding light on the molecular processes involved.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Physical
T. Schoenfeldova, H. Okur, V. Vezocnik, I. Iacovache, C. Cao, M. Dal Peraro, P. Macek, B. Zuber, S. Roke
Summary: This study demonstrates an ultrasensitive method for direct detection of protein binding to liposome membranes using high throughput second harmonic scattering (SHS). The approach allows label-free and noninvasive probing of protein-membrane interactions at sub-picomolar concentrations in 3D systems.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Analytical
Benjamin Charron, Alexandre Delorme, Caroline Dubois, Maryam Hojjat Jodaylami, Jean-Francois Masson
Summary: This study reveals the significant impact of human serum albumin (HSA) on the response and binding constants of sensing assays, while bovine serum albumin (BSA) does not show the same effect. The results also highlight the importance of the matrix protein/analyte/receptor interactions in determining the assay response.
Article
Physics, Applied
Kishan Jayanand, Anupama B. Kaul
Summary: The study explores the plasmonic and excitonic interactions in two-dimensional (2D) semiconductors and zero-dimensional (0D) metal nanoparticles, revealing the tunability of the spectral response. The geometry of the hybrid structures affects the surface plasmon resonance (SPR) peaks, leading to a shift in the spectral response of the 2D semiconductor and influencing the carrier dynamics.
APPLIED PHYSICS LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Maciej Trzaskowski, Marcin Drozd, Tomasz Ciach
Summary: This study investigated the attachment process of saccharide particles to cell receptors responsible for glucose transport across the cell membrane. The interactions between simple saccharides, such as glucose and sucrose, and dextran nanoparticles with two species of GLUT proteins were measured. The results showed that polysaccharide nanoparticles interacted with GLUT proteins, and the strengths of interactions varied between proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Edwin J. Ortiz-Riano, Diana L. Mancera-Zapata, Martha Ulloa-Ramirez, Fernando Arce-Vega, Eden Morales-Narvaez
Summary: Macromolecular association is important in biomedical sciences. This study presents a real-time biosensing platform using graphene oxide-modified microwell plates and fluorophore-labeled proteins to determine protein-binding constants. The platform proves to be comparable and consistent with existing methods in determining protein kinetics constants.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Ben Tadgell, Luis M. Liz-Marzan
Summary: Chiral plasmonic nanoparticles interact with biomolecules in distinct ways and can be probed using circular dichroism spectroscopy, offering potential advantages in biosensing and biomedical applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Analytical
Gizem Erol, Patricia Perez Schmidt, Alessia Pancaro, Javier M. Melo Diaz, Africa G. Barrientos, John Porter, Laura Polito, Michal Szymonik, Inge Nelissen, Daniel I. R. Spencer, Arianna Piotti, Marten Beeg, Maria-Grazia De Simoni, Stefano Fumagalli, Marco Gobbi
Summary: Circulating mannose-binding lectin (MBL) plays a crucial role in tissue damage caused by ischemic events. A new surface plasmon resonance (SPR) assay was developed to screen compounds inhibiting the binding of MBL to damaged endothelium. The assay successfully detected MBL binding and identified inhibitory effects of mannose, mannose residues carrying glycan, and mannose-coated gold nanoparticles.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Chemical
Aosheng Chang, Ben Niu, Jia Liu, Haoran Han, Zhibing Zhang, Wei Wang
Summary: Surface nanobubbles are spontaneously formed at the interface between hydrophobic surfaces and aqueous solutions, and their lifetimes are much longer than those predicted by classical thermodynamics model. This study used surface plasmon resonance microscopy (SPRM) to quantitatively measure the dissolution kinetics of individual surface nanobubbles in real time and investigated the effects of ionic strength and pH value on the dissolution rates (lifetime) of nanobubbles. The results showed that nanobubbles could persist for a long time in low-concentration electrolyte solutions or high-concentration non-electrolyte solutions, but dissolved quickly in high-concentration electrolyte solutions. Furthermore, the dissolution rates of surface nanobubbles increased with increasing ionic strength and acidity or alkalinity of the solution.
Article
Chemistry, Multidisciplinary
Ziwen Jiang, Yu-Hsuan Kuo, Mengqi Zhong, Jianchao Zhang, Xin X. Zhou, Lijuan Xing, James A. Wells, Yanzhuang Wang, Michelle R. Arkin
Summary: Protein-protein interactions (PPIs) play crucial roles in cellular signaling and functions. Precise modulation of PPIs can help understand their roles in cellular events and be therapeutically valuable. In this study, an antibody fragment-based modulator for the PPI between p97 and its adaptor protein NSFL1C was developed, demonstrating its potential application in therapeutic interventions by disrupting specific intracellular PPIs.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)