期刊
JOURNAL OF MOLECULAR NEUROSCIENCE
卷 51, 期 2, 页码 602-614出版社
HUMANA PRESS INC
DOI: 10.1007/s12031-013-0033-5
关键词
Micturition; Sensory transducer; Growth factor; Q-PCR; Western blot
资金
- National Institutes of Health (NIH) [DK051369, DK060481, DK065989]
- National Center for Research Resources [5 P30 RR 032135]
- National Institute of General Medical Sciences from the NIH [8 P30 GM 103498]
- Center of Biomedical Research Excellence (COBRE) in Neuroscience
- National Center for Research Resources of the NIH
Transient receptor potential vanilloid (TRPV) family member 4 (TRPV4) expression has been demonstrated in urothelial cells and dorsal root ganglion (DRG) neurons, and roles in normal micturition reflexes as well as micturition dysfunction have been suggested. TRP channel expression and function is dependent upon target tissue expression of growth factors. These studies expand upon the target tissue dependence of TRPV4 expression in the urinary bladder and lumbosacral DRG using a recently characterized transgenic mouse model with chronic overexpression of nerve growth factor (NGF-OE) in the urothelium. Immunohistochemistry with image analyses, real-time quantitative polymerase chain reaction, and Western blotting were used to determine TRPV4 protein and transcript expression in the urinary bladder (urothelium + suburothelium, detrusor) and lumbosacral DRG from littermate wild-type (WT) and NGF-OE mice. Antibody specificity controls were performed in TRPV4(-/-) mice. TRPV4 transcript and protein expression was significantly (p a parts per thousand currency signaEuro parts per thousand 0.001) increased in the urothelium + suburothelium and suburothelial nerve plexus of the urinary bladder and in small- and medium-sized lumbosacral (L1, L2, L6-S1) DRG cells from NGF-OE mice compared to littermate WT mice. NGF-OE mice exhibit significant (p a parts per thousand currency signaEuro parts per thousand 0.001) increases in NGF transcript and protein in the urothelium + suburothelium and lumbosacral DRG. These studies demonstrate regulation of TRPV4 expression by NGF in lower urinary tract tissues. Ongoing studies are characterizing the functional roles of TRPV4 expression in the sensory limb (DRG, urothelium) of the micturition reflex.
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