Article
Biochemistry & Molecular Biology
Mafalda S. L. Aresta Branco, Alejandro Gutierrez Cruz, Mahsa Borhani Peikani, Violeta N. Mutafova-Yambolieva
Summary: The urinary bladder requires proper concentrations of extracellular ATP and other purines at receptor sites for normal functioning. Ectonucleotidases play a crucial role in dephosphorylation of ATP to ADP, AMP and ADO. The activation of metabolic pathways can restrict the release of ectonucleotidases and prevent excessive hydrolysis of ATP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jin Kono, Masakatsu Ueda, Atsushi Sengiku, Sylvia O. Suadicani, Osamu Ogawa, Hiromitsu Negoro
Summary: The study suggests that urothelial Cx43 mediated ATP signaling and coordination of urothelial activity are essential for proper perception and regulation of responses to bladder distension in the animals' awake, active phase. This research highlights the significant role of Cx43 in bladder function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alejandro Gutierrez Cruz, Mafalda S. L. Aresta Branco, Mahsa Borhani Peikani, Violeta N. Mutafova-Yambolieva
Summary: The bladder urothelium and suburothelium/lamina propria play important roles in sensory and transducer functions, with the involvement of afferent neurons and urothelium-derived purine mediators. The effective concentrations of purines at receptor targets depend on the degradation of ATP by ectonucleotidases. This study explores the interactions between peptidergic and purinergic signaling mechanisms in the bladder and suggests that they have an impact on bladder excitability and functions by regulating the concentrations of adenine purines at effector cells in the lamina propria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jia-Fong Jhang, Han-Chen Ho, Yuan-Hsiang Hsu, Yuan-Hong Jiang, Hann-Chorng Kuo
Summary: This study found that patients with recurrent urinary tract infection (rUTI) have bladder defects and elevated urine inflammatory markers. After treatment with platelet-rich plasma (PRP) injections, some patients showed improvement in bladder defects.
Article
Pharmacology & Pharmacy
Zhinoos Taidi, Tommy Zhou, Kate H. Moore, Kylie J. Mansfield, Lu Liu
Summary: Inflammatory conditions of the urinary bladder are associated with urothelial damage and loss of function. The purinergic P2X7 receptor plays a role in such inflammatory conditions and may be a new therapeutic target for bladder inflammation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Neurosciences
Christopher H. Fry, Karen D. McCloskey
Summary: This passage summarizes the research on the role of ATP and purines in urinary tract physiology over the past fifty years, focusing on the modulation of urinary tract function by purines and purinergic signaling mechanisms.
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
(2021)
Article
Food Science & Technology
Hodan Ibrahim, Jacquie Maignel, Fraser Hornby, Donna Daly, Matthew Beard
Summary: This review discusses the current evidence of BoNT/A's effect on bladder sensation, potential mechanisms by which it exerts these effects, and recent advances in understanding its action in bladder tissue.
Article
Physiology
B. Malique Jones, Gerald C. Mingin, Nathan R. Tykocki
Summary: Mast cells and degranulation of inflammatory mediators contribute to lower urinary tract symptoms. This study found that the mast cell stimulator compound 48/80 alters urinary bladder smooth muscle contractility via urothelium-dependent inflammatory pathways, independent of mast cells. These findings suggest that compound 48/80 could be used to manage inflammatory nonneurogenic bladder hyperactivity.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Physiology
Mafalda S. L. Aresta Branco, Alejandro Gutierrez Cruz, Jacob Dayton, Brian A. Perrino, Violeta N. Mutafova-Yambolieva
Summary: Prior studies indicate that ATP released by the urothelium plays an important role in bladder mechanotransduction and undergoes hydrolysis during bladder filling. Experimental results show that the hydrolysis of ATP and ADP is significantly greater in distended bladders compared to non-distended bladders, while the hydrolysis of AMP remains unchanged. Furthermore, the activity of nucleotidases is also higher in the extraluminal solution from distended bladders. These findings suggest that the hydrolysis reaction during bladder filling is mechanosensitive.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alejandro Gutierrez Cruz, Mafalda S. L. Aresta Branco, Brian A. Perrino, Kenton M. Sanders, Violeta N. Mutafova-Yambolieva
Summary: Adenosine 5'-triphosphate (ATP) is released in the bladder lumen during filling to regulate bladder excitability. Increased urinary ATP is suggested as a biomarker for bladder dysfunctions including overactive bladder, interstitial cystitis, and bladder pain syndrome. Altered urinary ATP might also be associated with voiding dysfunctions linked to disease states associated with metabolic syndrome.
Article
Immunology
Nicole M. Gilbert, Valerie P. O'Brien, Chevaughn Waller, Ekatherina Batourina, Cathy Lee Mendelsohn, Amanda L. Lewis
Summary: Gardnerella, a common member of the female urinary microbiome, is associated with urological disorders in women. In a mouse model, Gardnerella inoculation leads to urothelial exfoliation and activation of pathways involved in inflammation and immunity. Bladder exposure to Gardnerella also increases susceptibility to subsequent urinary tract infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Natalia Zeber-Lubecka, Maria Kulecka, Katarzyna Zaleska-Oracka, Michalina Dabrowska, Aneta Balabas, Ewa E. Hennig, Magdalena Szymanek-Szwed, Michal Mikula, Beata Jurkiewicz, Jerzy Ostrowski
Summary: The aim of this study is to determine the molecular differences between the bladder body and trigonal urothelia. The analysis of transcriptomes and microbiomes revealed functional differences between the two regions and significant differences in bacterial taxa in urine.
Review
Biochemistry & Molecular Biology
Xiang Xie, Jiayu Liang, Run Huang, Chuang Luo, Jiali Yang, Hongming Xing, Le Zhou, Han Qiao, Erti Ergu, Huan Chen
Summary: Ketamine cystitis is a chronic bladder inflammation caused by multiple tissue injuries in the bladder, including urothelium disruption, lamina propria fibrosis and inflammation, microvascular injury, neuropathological alterations, and bladder smooth muscle abnormalities. Ketamine induces these tissue injuries by regulating different signaling pathways, contributing to the pathogenesis of ketamine cystitis.
Article
Biochemistry & Molecular Biology
Emma Mitidieri, Annalisa Pecoraro, Erika Esposito, Vincenzo Brancaleone, Carlotta Turnaturi, Luigi Napolitano, Vincenzo Mirone, Ferdinando Fusco, Giuseppe Cirino, Raffaella Sorrentino, Giulia Russo, Annapina Russo, Roberta dEmmanuele di Villa Bianca
Summary: The urothelium plays an important role in bladder homeostasis through the hydrogen sulfide pathway and beta(3) adrenoceptor-mediated relaxation. The study demonstrates the relevance of urothelial beta(3) adrenoceptors in regulating bladder tone and supports the use of beta(3) agonists in patients with an overactive bladder.
Review
Biochemistry & Molecular Biology
Xu Li, Junwei Hu, Xuedan Zhao, Juanjuan Li, Yuelai Chen
Summary: Understanding the role of piezo channel proteins in the urinary system could lead to novel treatments for urinary system diseases. These proteins convert mechanical signals into biological signals and mutations or disruption to them may trigger diseases. Further investigations are needed to fully understand their mechanisms of action.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
