Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Agricultural Engineering
Yang Zhang, Xiuling Zhou, Yingying Yao, Qianqian Xu, Haiying Shi, Kuiming Wang, Wei Feng, Yanbing Shen
Summary: Coexpression of Vitreoscilla hemoglobin and sterol transporter ATPase genes in Mycobacterium sp. was found to improve androstenone production. Immobilization on bagasse increased productivity significantly.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Ting Ding, Yong Fan, Er-Ying Jiang, Xiao-Yi Shi, Eike Krautter, Guang-Rong Hu, Fu-Li Li
Summary: This study successfully improved the growth rate and eicosapentaenoic acid content of N. oceanica cells by introducing the Vitreoscilla hemoglobin gene, while reducing ROS levels and photorespiration levels. These results indicate that Vitreoscilla hemoglobin can enhance microalgal growth under stress conditions by mitigating ROS damage and modulating photorespiration.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yaning Xu, Nan Zhao, Fengxi Li, Chunyu Wang, Hanqing Xie, Junhao Wu, Lei Cheng, Lei Wang, Zhi Wang
Summary: An efficient and eco-friendly method for the synthesis of benzoxazoles catalyzed by Vitreoscilla hemoglobin (VHb) in water at room temperature was reported. The annulation reaction of phenols with amines in the presence of VHb yielded 27 functionally diverse benzoxazoles in moderate to high yields (62%-94%) within 1 hour. This work expands the application of hemoglobin in organic synthesis.
Article
Biotechnology & Applied Microbiology
Jun-feng Li, Hong-fang Li, Shu-min Yao, Meng-juan Zhao, Wen-xun Dong, Sheng-kang Liang, Xing-yong Xu
Summary: The study focused on enhancing the oxygen utilization efficiency and production of Sophorolipids by introducing the Vitreoscilla hemoglobin (VHb) gene into S. bombicola. The results showed improved SLs production in shake flask culture and a higher DO level in the fermentation broth in a 5 L bioreactor. VHb expression also upregulated key genes related to electron transfer, TCA cycle, and SL synthesis, leading to enhanced biosynthesis of SLs and intracellular oxygen utilization efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Yaning Xu, Nan Zhao, Fengxi Li, Hanqing Xie, Junhao Wu, Chunyu Wang, Zhengqiang Li, Lei Wang
Summary: We reported a method for the synthesis of 2-aryl benzothiazoles catalyzed by Vitreoscilla hemoglobin (VHb). This method has the advantages of high efficiency, mild reaction conditions, and wide substrate scope.
MOLECULAR CATALYSIS
(2022)
Review
Microbiology
Fei Yu, Xinrui Zhao, Ziwei Wang, Luyao Liu, Lingfeng Yi, Jingwen Zhou, Jianghua Li, Jian Chen, Guocheng Du
Summary: Vitreoscilla hemoglobin (VHb) is a widely applied soluble heme-binding protein in the field of biotechnology due to its ability to enhance cell growth, product synthesis, and stress tolerance. Particularly effective under oxygen-limited conditions, VHb can interact with terminal oxidase to deliver enough oxygen for high-cell-density fermentation. The development of bioinformatics and synthetic biology has led to the discovery of new physicochemical properties and metabolic regulatory effects of VHb, enhancing its expression levels and expanding its applications.
Article
Biotechnology & Applied Microbiology
Karim E. Jaen, Daniela Velazquez, Juan-Carlos Sigala, Alvaro R. Lara
Summary: This study demonstrated that expressing Vitreoscilla hemoglobin (VHb) in E. coli chromosome can improve plasmid DNA (pDNA) production in biphasic fed-batch cultures by reducing acetate accumulation and increasing pDNA yields. The presence of VHb also led to higher fluxes in anaplemtic pathways and increased lactate production in cells under microaembic conditions. Despite lower RNAII/RNAI ratio, VHb-expressing strain achieved a significantly higher final pDNA titer compared to the wild type strain.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Zi-Xu Wang, Na Li, Jun-Wei Xu
Summary: Efficient expression of the Vitreoscilla hemoglobin gene in G. lucidum increased the production of exopolysaccharides and enhanced their antioxidant activity, without changing their monosaccharide composition or molecular weight distribution.
Article
Biotechnology & Applied Microbiology
Yan Du, Fangyu Cheng, Miaomiao Wang, Chunmeng Xu, Huimin Yu
Summary: By utilizing a combination of strategies, the yield of hyaluronic acid was successfully increased, achieving a high titer in fed-batch culture, laying the foundation for future industrial production using engineered Corynebacterium glutamicum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qingzhuo Wang, Huabao Zheng, Rongsheng Tao, Qi Li, Yu Jiang, Sheng Yang
Summary: VHb can enhance the catalytic activity of oxidases under hypoxic conditions, suggesting its potential importance in industrial applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yaning Xu, Fengxi Li, Nan Zhao, Jiali Su, Chunyu Wang, Ciduo Wang, Zhengqiang Li, Lei Wang
Summary: This study demonstrates the synthesis of 2-aminobenzoxazoles and 2-aminobenzothiazoles using an artificial Vitreoscilla hemoglobin (VHb) with cobalt porphyrin, providing a green and efficient method. The porphyrin ligand substitution strategy is shown to enhance the catalytic properties of hemoproteins in non-natural reactions.
Article
Biotechnology & Applied Microbiology
Qing Zhang, Yaozhong Chen, Lin Gao, Jian'gang Chen, Xin Ma, Dongbo Cai, Dong Wang, Shouwen Chen
Summary: This study optimized the expression cassette of VHB for the production of gamma-PGA, resulting in increased yields and improved oxygen transfer and ATP generation. The study also identified a suitable promoter for VHB expression and gamma-PGA synthesis, leading to further increases in gamma-PGA yield. Overall, this study achieved an efficient VHB expression cassette for oxygen transfer and gamma-PGA synthesis, with potential applications in the production of other metabolites.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Review
Microbiology
Dale A. Webster, Kanak L. Dikshit, Krishna R. Pagilla, Benjamin C. Stark
Summary: In 1986, the discovery of hemoglobin (VHb) in Vitreoscilla bacteria expanded the scope of oxygen binding proteins. Research in the following years focused on its biochemical properties, genetic sequence, and expression control, leading to practical applications in microbial hosts. VHb's pioneering role in bacterial hemoglobin research and its ongoing biotechnological applications have made it a topic of interest 35 years after its discovery.
Review
Environmental Sciences
N. Nivetha, B. Srivarshine, B. Sowmya, Mangaiyarkarasi Rajendiran, Panchamoorthy Saravanan, R. Rajeshkannan, M. Rajasimman, Thi Hong Trang Pham, VenkatKumar Shanmugam, Elena-Niculina Dragoi
Summary: Pollution of heavy metals is a risky contamination that should be managed for public health concerns. Bioremediation is a bio-mechanism where organic entities reuse and transform wastes into a less harmful substance. This review discusses the sources, toxicity, effects, and bioremediation methods of five heavy metals: lead, mercury, arsenic, chromium, and manganese. The concentrations here are on biosorption methods, microbial utilization, algae, and fungi in bioremediation.
Article
Biotechnology & Applied Microbiology
Stephanie A. Kunkel, Krishna R. Pagilla, Benjamin C. Stark
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Review
Biotechnology & Applied Microbiology
Benjamin C. Stark, Krishna R. Pagilla, Kanak L. Dikshit
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Agricultural Engineering
Bhargavi Subramanian, Alexandre Miot, Bonnie Jones, Corey Klibert, Krishna R. Pagilla
BIORESOURCE TECHNOLOGY
(2015)
Review
Biophysics
Bhargavi Subramanian, Krishna R. Pagilla
COLLOIDS AND SURFACES B-BIOINTERFACES
(2015)
Article
Engineering, Civil
Sara Glade, Krishna Pagilla
JOURNAL AMERICAN WATER WORKS ASSOCIATION
(2015)
Article
Engineering, Environmental
Andrew Shaw, Imre Takacs, Krishna Pagilla, Rumana Riffat, Haydee DeClippeleir, Christopher Wilson, Sudhir Murthy
WATER ENVIRONMENT RESEARCH
(2015)
Article
Engineering, Environmental
Giridhar Prabhukumar, Gurmanpreet Singh Bhupal, Krishna R. Pagilla
WATER ENVIRONMENT RESEARCH
(2015)
Article
Engineering, Environmental
Chris Maher, J. B. Neethling, Sudhir Murthy, Krishna Pagilla
Article
Engineering, Environmental
M. Arnaldos, K. R. Pagilla
WATER SCIENCE AND TECHNOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Stephanie A. Kunkel, Krishna R. Pagilla, Benjamin C. Stark
Article
Environmental Sciences
Laura Haak, Ratul Roy, Krishna Pagilla
Article
Biochemistry & Molecular Biology
Ratul Roy, Laura Haak, Lin Li, Krishna Pagilla
PROCESS BIOCHEMISTRY
(2016)
Review
Engineering, Environmental
Lin Li, Krishna R. Pagilla
Article
Engineering, Environmental
Yanming Zhang, Demetrios Moschandreas, Krishna Pagilla
WATER SCIENCE AND TECHNOLOGY
(2014)
Article
Engineering, Environmental
Tayebeh Goli, Kamrun Ahmadi, Krishna R. Pagilla, Eric A. Marchand
Summary: This study investigated the removal of dissolved organic nitrogen (DON) in reclaimed water through enhanced coagulation, ozonation, and ozonation/biodegradation at both lab and pilot scales. The results showed that DON could be effectively removed by enhanced coagulation, ozonation, and ozonation/biodegradation, with the best results achieved by the combination of ozonation and biodegradation.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)