Review
Biology
Akihiro Yasoda
Summary: C-type natriuretic peptide (CNP) is an important member of the natriuretic peptide family, with significant physiological and pathophysiological importance in the heart. Together with its receptor NPR-B, CNP is considered the major heart-protective natriuretic peptide in heart failure.
Review
Biology
Yasuaki Nakagawa, Toshio Nishikimi
Summary: CNP is the third natriuretic peptide to be discovered and is widely expressed in the central nervous system, osteochondral system, and vascular system. It acts mainly through GC-B receptor, which differs from the receptors targeted by ANP and BNP. Research has shown that CNP attenuates cardiac remodeling, regulates vascular function, and has therapeutic potential for cardiovascular diseases.
Article
Pharmacology & Pharmacy
Leonie Berten-Schunk, Yvonne Roger, Heike Bunjes, Andrea Hoffmann
Summary: The design of implants for tissue transitions is challenging, and the rotator cuff in the shoulder is an example of such a transition. Electrospun fiber mats of PCL loaded with CS/TPP nanoparticles and TGF-beta(3) were used to optimize the implant for enthesis regeneration. Higher loading concentrations of TGF-beta(3) led to increased release, larger cell pellets, and enhanced chondrogenic differentiation of MSCs.
Article
Biotechnology & Applied Microbiology
Chantal Voskamp, Wendy J. L. M. Koevoet, Gerjo J. V. M. Van Osch, Roberto Narcisi
Summary: This study investigated the impact of senescence and the senescence-associated secretory phenotype (SASP) on chondrogenic differentiation of MSCs. Inducing senescence in cells during expansion or early differentiation significantly reduced cartilage matrix formation. However, inducing senescence at a later stage of differentiation did not significantly affect chondrogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhenwei Zhou, Jinghong Zhong, Jingcheng Zhang, Jie Yang, Xiangyang Leng, Baojin Yao, Xukai Wang, Haisi Dong
Summary: Deer TGF-beta 1 promotes chondrogenesis by regulating chondrocyte proliferation, differentiation, and ECM synthesis. This study provides insights into the molecular mechanisms underlying the effects of deer TGF-beta 1 on chondrocyte viability.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Mingyan Zhao, Xiang Gao, Jinsong Wei, Chenlin Tu, Hong Zheng, Kaipeng Jing, Jiaqi Chu, Wei Ye, Thomas Groth
Summary: Polyelectrolyte multilayer (PEM) surface coatings composed of nHA and type I collagen can promote adhesion, growth, and chondrogenic differentiation of hUC-MSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
Hong-Bin Yu, Jing Xiong, Hui-Zhen Zhang, Qin Chen, Xu-Yong Xie
Summary: Treating tendon fibrosis with TGF beta 1-transfected TSCs may be an effective approach.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Safak Chasan, Eliane Hesse, Passant Atallah, Matthias Gerstner, Solvig Diederichs, Astrid Schenker, Kay Grobe, Carsten Werner, Wiltrud Richter
Summary: This study demonstrates the crucial role of GAG sulfation as a niche instruction signal in determining the fate of chondral stem cells by silencing prohypertrophic pathways, providing the first proof-of-principle on how GAG modification-patterns can determine cell lineage-choice during tissue morphogenesis in vivo.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Yann D. Ladner, Hermann Kasper, Angela R. Armiento, Martin J. Stoddart
Summary: This paper presents a new, simple-to-build and operate, multi-well kinematic load bioreactor and explores its impact on the chondrogenic differentiation of human bone marrow-derived stem cells. The bioreactor activates transforming growth factor beta 1, upregulates chondrogenic genes, and increases sulfated glycosaminoglycan retention within the scaffolds, thereby accelerating and improving the testing of cells, new biomaterials, and tissue-engineered constructs.
Article
Multidisciplinary Sciences
Maxwell Landolina, Anne Yau, Yupeng Chen
Summary: This study successfully designed a DNA-inspired Janus-based nanomatrix (JBNm) with a highly localized microenvironment and layer-by-layer structure for self-sustainable cartilage tissue constructs.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Behrouz Baghaiee, Roshanak Bayatmakoo, Pouran Karimi, Linda Shannon Pescatello
Summary: The study demonstrated that aerobic training effectively inhibits the calcineurin/NFATc pathway in middle-aged rats, leading to improvements in intracellular Ca2+ levels and related factors in the heart tissue.
Article
Biology
Chizuru Sogawa-Fujiwara, Yasuhiro Fujiwara, Atsuki Hanagata, Qunhui Yang, Taiki Mihara, Noriyuki Kaji, Tetsuo Kunieda, Masatoshi Hori
Summary: Histological observation revealed clear dilation of blood vessels in the mesentery of preweaning Npr2(slw/slw) mice. Additionally, lacteals were partially dilated or randomly oriented in the villi, and mesenteric adipocytes were undeveloped in these mice. These findings provide valuable insights into the effects of CNP/NPR-B signal deficiency on the intestine with mesentery.
BMC RESEARCH NOTES
(2021)
Article
Biochemical Research Methods
Kyungsoo Kim, Young Sik Kim, Ju Woong Jang, Gyun Min Lee
Summary: In this study, the production of recombinant human transforming growth factor-beta1 (rhTGF-beta 1) in Chinese hamster ovary (CHO) cells was enhanced through characterization and optimization. It was found that mature rhTGF-beta 1 was mostly produced in the form of latent rhTGF-beta 1 in CHO-rhTGF-beta 1 cells, and a significant amount of unprocessed precursors was also produced. Overexpression of PACEsol in CHO-rhTGF-beta 1 cells effectively cleaved the unprocessed precursors, resulting in increased production of mature rhTGF-beta 1.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Medicine, Research & Experimental
Nicole K. Patel, Johanna H. Nunez, Michael Sorkin, Simone Marini, Chase A. Pagani, Amy L. Strong, Charles D. Hwang, Shuli Li, Karthik R. Padmanabhan, Ravi Kumar, Alec C. Bancroft, Joey A. Greenstein, Reagan Nelson, Husain A. Rasheed, Nicholas Livingston, Kaetlin Vasquez, Amanda K. Huber, Benjamin Levi
Summary: The study demonstrates the critical role of autocrine TGF-beta 1 signaling in macrophages in heterotopic ossification (HO) after traumatic injury. The findings highlight the importance of the TGF-beta 1/ALK5 signaling pathway in HO formation.
Article
Medicine, Research & Experimental
Lu Feng, Zhengmeng Yang, Yucong Li, Nan Hou, Boguang Yang, Xuan Lu, Shanshan Bai, Ming Wang, Xiaoting Zhang, Haixing Wang, Yaofeng Wang, Micky D. Tortorella, Gang Li
Summary: This study identified a small molecule, docosahexaenoic acid (DHA), with therapeutic potential for osteoarthritis (OA) treatment. DHA exhibited anti-inflammatory and chondroprotective effects in OA chondrocytes and promoted chondrogenesis in bone marrow-derived mesenchymal stem cells (BMSCs) impaired by IL-1 beta. The key mediator of DHA's effects was found to be Malat1. Additionally, DHA rescued cartilage loss and damage in a surgery-induced OA mice model. These findings suggest that DHA may be a potential drug candidate for OA patients with elevated MALAT1 expression levels.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Orthopedics
Alp Akman, Fahir Demirkan, Nuran Sabir, Murat Oto, Cagdas Yorukoglu, Esat Kiter
INDIAN JOURNAL OF ORTHOPAEDICS
(2017)
Article
Neurosciences
Sema Serter Kocoglu, Duygu Gok Yurtseven, Cihan Cakir, Zehra Minbay, Ozhan Eyigor
Article
Neurosciences
Duygu Gok Yurtseven, Sema Serter Kocoglu, Zehra Minbay, Ozhan Eyigor
Article
Anatomy & Morphology
S. Serter Kocoglu, C. Cakir, Z. Minbay, O. Eyigor
Summary: This study demonstrated the expression of glutamate receptor subunits in neuronostatin neurons, suggesting the formation of functional receptor complexes. These findings provide important clues for further research on the function and regulation mechanisms of neuronostatin neurons.
FOLIA MORPHOLOGICA
(2022)
Review
Cell & Tissue Engineering
Ahmet Cevik Tufan
Summary: The Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China in December 2019 and has since spread globally. Treatments are currently supportive, with ongoing investigations into potential drug discovery. Recent developments have suggested the use of Mesenchymal Stem/Stromal Cells (MSCs) as a potential therapy, with clinical trials and case studies being performed to evaluate their efficacy in treating COVID-19.
CURRENT STEM CELL RESEARCH & THERAPY
(2021)
Article
Anatomy & Morphology
S. Serter Kocoglu, C. Oy, Z. Halk, C. Cakir, Z. Minbay, O. Eyigor
Summary: This study found that refeeding and intraperitoneal glucose injection can activate Nesfatin-1 neurons, and this effect may be regulated by the glutamatergic system. These findings are important for further understanding of satiety regulation and glucose metabolism.
FOLIA MORPHOLOGICA
(2022)
Article
Oncology
Sema Serter Kocoglu, Mucahit Secme, Fatma Bahar Sunay
Summary: The study aimed to investigate the anticancer effect of erianin and its underlying mechanism on SH-SY5Y cells. The results showed that erianin significantly inhibited cell proliferation, invasion, and migration, induced apoptosis through increased expression of caspase-7, FADD, BID, and DR5 genes. Additionally, erianin significantly decreased the expression of hsa-miR-155-5p and hsa-miR-223-3p. Erianin may be a natural, safe, and easily accessible drug candidate for neuroblastoma treatment.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2023)
Article
Infectious Diseases
Sema Serter Kocoglu, Fatma Bahar Sunay, Pakize Nur Akkaya
Summary: This study investigated the therapeutic effects of single and combined monensin and rapamycin treatments on mTOR signaling pathway-mediated apoptosis and tumor growth in a neuroblastoma cell xenograft model. The combination of monensin and rapamycin reduced tumor growth, triggered apoptosis, and suppressed the expression of PI3K/AKT/mTOR. These preclinical results may pave the way for developing new therapeutic approaches to treat neuroblastoma.
Article
Infectious Diseases
Sema Serter Kocoglu, Muecahit Secme, Ceren Oy, Gozde Korkusuz, Levent Elmas
Summary: This study aimed to investigate the antiproliferative effects of monensin alone and in combination with rapamycin on neuroblastoma cells mediated by the PI3K/AKT signaling pathway. The results showed that monensin exerts an antiproliferative effect by targeting the PI3K/AKT signaling pathway in neuroblastoma cells. It is suggested that monensin and its combination with rapamycin may be an effective therapeutic candidate for treating neuroblastoma.
Article
Medicine, Research & Experimental
Sema Serter Kocoglu, Ceren Oy, Muecahit Secme, F. Bahar Sunay
Summary: Monensin inhibits the growth, invasion, migration, and colony formation of neuroblastoma cells, and induces apoptosis in these cells. It increases the expression of caspase-3, 7, 8, and 9, and decreases the expression of Bax and Bcl-2. This study suggests that monensin could be a safe and effective therapeutic candidate for pediatric neuroblastoma.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Ceyhun Caglar, Serhat Akcaalan, Yasemin Akcaalan, Gulben Akcan, Ahmet Cevik Tufan, Mustafa Akkaya, Metin Dogan
Summary: The effects of tranexamic acid (TXA) on the knee's articular cartilage, ACL, and joint capsule were evaluated histologically. The results showed that IV administration of TXA damages the cartilage and ACL, while IA administration of TXA is more protective.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Serhat Akcaalan, Gulben Akcan, Ahmet Cevik Tufan, Ceyhun Caglar, Yasemin Akcaalan, Mustafa Akkaya, Metin Dogan
Summary: The experiment aimed to examine the effects of tranexamic acid, a drug commonly used to control bleeding, on the hip joint and sciatic nerve in animal experiments. Three groups of rats were used, with topical saline injections, topical tranexamic acid injections, and intravenous tranexamic acid injections. Histological analysis of samples taken from the hip joint showed significantly more cartilage degradation in the femoral head in both the intravenous and intraarticular tranexamic acid groups compared to the control group. No difference was found in acetabular cartilage. The experiment also showed that tranexamic acid had negative effects on hip joint cartilage thickness.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Oncology
Mucahit Secme, Sema Serter Kocoglu
Summary: This study aimed to investigate the TLR4/IRF3-mediated antiproliferative and anti-inflammatory effects of monensin in colorectal cancer cells. The results showed that monensin inhibited the proliferation of colorectal cancer cells and reduced the mRNA expression levels of TLR4 and IRF3 genes. This study demonstrates for the first time the TLR4/IRF3-mediated anti-inflammatory effects of monensin in colorectal cancer cells.
Article
Biology
Sema Serter Kocoglu, Mucahit Secme, Levent Elmas
Summary: Erianin may serve as a potential therapeutic component for treating glioblastoma, exhibiting strong anti-tumor and pro-apoptotic effects.
ARCHIVES OF BIOLOGICAL SCIENCES
(2022)
Article
Orthopedics
Ahmet Cevik Tufan
JOURNAL OF ORTHOPAEDICS
(2019)
