Article
Biochemistry & Molecular Biology
Christine Njiru, Corinna Saalwaechter, Oliver Gutbrod, Sven Geibel, Nicky Wybouw, Thomas Van Leeuwen
Summary: This study reports the discovery of a highly pyflubumide resistant population of the spider mite Tetranychus urticae with a H258Y substitution in subunit B of SDH. The mutation has a significant impact on the toxicity of SDH inhibitors and causes cross-resistance between cyenopyrafen and pyflubumide.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Environmental Sciences
Yifan Zhang, Zhiqiang Kong, Noel Gregoire, Lin Li, Lin Yang, Mengying Zhao, Nuo Jin, Fengzhong Wang, Bei Fan, Frederic Francis, Minmin Li
Summary: This study investigated the effects of CYF on non-target (honeybees) and target (bee mites and red spider mites) organisms. It was found that (+)-CYF had a stronger negative effect on cell viability compared to (-)-CYF and rac-CYF in MCF-7 cells. Acute toxicity analysis showed that honeybees had low toxicity to CYF, while bee mites and red spider mites were more sensitive to (+)-CYF. Proteomics profiling revealed potential CYF-targeted proteins in honeybees related to energy metabolism, stress responses, and protein synthesis.
Article
Food Science & Technology
Maria Anastassiadou, Giovanni Bernasconi, Alba Brancato, Luis Carrasco Cabrera, Lucien Ferreira, Luna Greco, Samira Jarrah, Aija Kazocina, Renata Leuschner, Jose Oriol Magrans, Ileana Miron, Stefanie Nave, Ragnor Pedersen, Hermine Reich, Alejandro Rojas, Angela Sacchi, Miguel Santos, Alessia Pia Scarlato, Anne Theobald, Benedicte Vagenende, Alessia Verani
Summary: Applicants BASF Agro B.V. and Certis Europe B.V. submitted requests to modify the existing maximum residue levels (MRLs) for cyflumetofen in various crops. The data supported MRL proposals for citrus fruits, apricots, peaches, tomatoes, aubergines, cucumbers, and hops, with risk assessment finding no health risks from consuming residues of cyflumetofen.
Article
Biochemistry & Molecular Biology
Yusuke Itoh, Yushi Shimotsuma, Akiya Jouraku, Wannes Dermauw, Thomas Van Leeuwen, Masahiro Osakabe
Summary: The study found that maternal effects and CYP activity were related to pyridaben resistance inheritance in T. urticae, with CYP392A3 potentially playing a key role in high resistance levels. Congenic strains with both mutant PSST and CYP392A3 displayed high resistance, indicating a synergistic or cumulative effect between the two genes. Further investigation is needed to uncover other potential factors influencing pyridaben resistance.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Ayumu Maeoka, Masahiro Osakabe
Summary: This study found that individual mutations of B-I260V or C-S56L alone did not confer high resistance levels to pyflubumide and cyenopyrafen in Tetranychus urticae, but the co-occurrence of these two mutations could result in very high resistance levels. The effects of mutations vary among complex II inhibitors.
PEST MANAGEMENT SCIENCE
(2021)
Review
Food Science & Technology
Giulia Bellisai, Giovanni Bernasconi, Alba Brancato, Luis Carrasco Cabrera, Lucien Ferreira, German Giner, Luna Greco, Samira Jarrah, Aija Kazocina, Renata Leuschner, Jose Oriol Magrans, Ileana Miron, Stefanie Nave, Ragnor Pedersen, Hermine Reich, Silvia Ruocco, Miguel Santos, Alessia Pia Scarlato, Anne Theobald, Benedicte Vagenende, Alessia Verani
Summary: EFSA reviewed the maximum residue levels for cyflumetofen and conducted a consumer risk assessment. While no apparent risk to consumers was identified, some information required by the regulatory framework was missing. Further consideration by risk managers is needed for some MRL proposals derived by EFSA.
Review
Infectious Diseases
Jessica Waldman, Guilherme Marcondes Klafke, Lucas Tirloni, Carlos Logullo, Itabajara da Silva Vaz Jr
Summary: Acaricide resistance is a global problem with economic and public health implications. The synganglion, the tick central nervous system, is a key target for acaricides and understanding its mode-of-action and resistance mechanisms can help develop new strategies for control.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Environmental Sciences
Jie Han, Qinglan Zheng, Danxi Xie, Anas Muhammad, Cem Isik
Summary: The study examines the relationship between green financing and high-quality economic development, and finds that green financing can improve high-quality economic development. The research also highlights the importance of government governance and market synergy in promoting green finance for high-quality economic development. The impact of green finance on high-quality economic development is heterogeneous, with green credit and green insurance having significantly higher impacts in the eastern regions of China.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Entomology
Delzuite Teles Leite, Roberto Barbosa Sampaio, Emerson Dechechi Chambo, Candida Maria Lima Aguiar, Mauricio Sekiguchi de Godoy, Carlos Alfredo Lopes de Carvalho
Summary: This study evaluated the toxic effects of chlorpyrifos, cyflumetofen, and difenoconazole on Tetragonisca angustula bees exposed in different ways. While cyflumetofen and difenoconazole caused lethal effects, surviving bees showed significant behavioral changes. Chlorpyrifos was extremely toxic to T. angustula in all exposure means, with difenoconazole showing less tolerance when applied via ingestion and on treated surfaces. All pesticides caused changes in bee behavior.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2022)
Article
Entomology
Nevriye Ozkan, Sibel Yorulmaz
Summary: This study investigates the development of pyridaben resistance in the predatory mite N. californicus, the inheritance of this resistance, and its association with target region mutations. The researchers found that after seven selections, N. californicus exhibited a 270-fold increase in pyridaben resistance. Inheritance studies revealed that pyridaben resistance in F1 females was due to incomplete dominance and sex. The mites resistant to pyridaben did not show significant differences in monooxygenase enzyme levels compared to the initial population. No amino acid changes were detected in the target site mutations of pyridaben-resistant N. californicus populations. The researchers suggest that the increased pyridaben resistance may be caused by mechanisms other than target site mutations.
INTERNATIONAL JOURNAL OF ACAROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tatiana Saporiti, Mauricio Cabrera, Josefina Bentancur, Maria Elisa Ferrari, Nallely Cabrera, Ruy Perez-Montfort, Francisco J. Aguirre-Crespo, Jorge Gil, Ulises Cuore, Dimitris Matiadis, Marina Sagnou, Guzman Alvarez
Summary: Rhipicephalus microplus, the common cattle tick, is a major ectoparasite in livestock causing significant economic and health losses. There is an urgent need to find alternatives for controlling resistant ticks since all currently available acaricides have reported resistance. This study discovered compounds derived from natural sources and synthetic organic molecules that showed potential as acaricidal agents against resistant ticks, with minimal toxicity to mammalian cells.
Article
Entomology
Mohammadreza Havasi, Ali R. Bandani, Azadeh Zahedi Golpayegani
Summary: The assessment of the long-term negative effects of acaricides on mites can determine their toxicity and side effects. Sublethal concentrations can affect populations at lower concentrations and provide insights into their sublethal effects. The study found that exposure to cyflumetofen affected the biological parameters of the mites, including their lifetime, fecundity, and longevity. These findings suggest the importance of using sublethal concentrations for integrated pest management programs and benefiting farmers and consumers.
SYSTEMATIC AND APPLIED ACAROLOGY
(2023)
Review
Agriculture, Dairy & Animal Science
Tom Strydom, Robert P. Lavan, Siddhartha Torres, Kathleen Heaney
Summary: Cattle parasites, such as intestinal roundworms, flatworms, and ticks, significantly impact the health and economic value of beef cattle. The losses incurred by beef cattle producers due to parasitism reach billions of dollars annually. Proper prevention and treatment are essential for maintaining healthy beef cattle, profitability, and meeting the global demand for sustainable protein and cattle products.
Article
Infectious Diseases
Adeyinka Fadahunsi, Christopher Kumm, Kirsty Graham, Adalbertoto A. Perez de Leon, Felix Guerrero, Oliver A. E. Sparagano, Robert D. Finn
Summary: The management of Rhipicephalus microplus, the cattle tick, is difficult due to its resistance to multiple acaricides. In this study, a cytochrome P450 oxidoreductase (CPR) from ticks was characterized biochemically. The findings suggest that targeting RmCPR could lead to the development of safer and more effective acaricides against R. microplus.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Yujun Han, Ying Sun, Hong Ma, Ruolin Wang, Yuning Lan, Haifeng Gao, Zhaofeng Huang
Summary: Wild oat is a common and problematic weed in wheat fields in China, and controlling it has become increasingly difficult. This study aimed to identify the molecular basis of clodinafop-propargyl resistance in wild oat. Two resistant populations were found to have high resistance to fenoxaprop-p-ethyl, low resistance to clethodim and pinoxaden, but they were sensitive to mesosulfuron-methyl and pyroxsulam. An Ile2041-Asn mutation was identified in both resistant plants. The resistant populations also showed metabolic resistance, which was confirmed by the application of inhibitors.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yusuke Itoh, Yushi Shimotsuma, Akiya Jouraku, Wannes Dermauw, Thomas Van Leeuwen, Masahiro Osakabe
Summary: The study found that maternal effects and CYP activity were related to pyridaben resistance inheritance in T. urticae, with CYP392A3 potentially playing a key role in high resistance levels. Congenic strains with both mutant PSST and CYP392A3 displayed high resistance, indicating a synergistic or cumulative effect between the two genes. Further investigation is needed to uncover other potential factors influencing pyridaben resistance.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Food Science & Technology
Laura Franz, Klaus Raming, Ralf Nauen
Summary: This study confirmed the importance of mutations in the extracellular loop 4 of the ATP-binding cassette transporter C2 for resistance to Cry1F in the fall armyworm.
Article
Biochemistry & Molecular Biology
Leily Daneshian, Isabella Renggli, Ryan Hanaway, Lesa R. Offermann, Caleb R. Schlachter, Shannon Henry, Rahul Prakash, Nicky Wybouw, Wannes Dermauw, Linda S. Shimizu, Thomas M. Makris, Vojislava Grbic, Miodrag Grbic, Maksymilian Chruszcz, Ricardo Hernandez Arriaza, Thomas Van Leeuwen
Summary: This study reveals the structure and function of TuCAS in Tetranychus urticae, showing its involvement in cyanide detoxification and additional enzymatic activities. The findings provide new molecular insights into the xenobiotic metabolism of this species.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Berdien De Beer, Ernesto Villacis-Perez, Mousaalreza Khalighi, Corinna Saalwaechter, Marilou Vandenhole, Wim Jonckheere, Ibrahim Ismaeil, Sven Geibel, Thomas Van Leeuwen, Wannes Dermauw
Summary: This study found that resistance to the organotin acaricide FBO in the spider mite Tetranychus urticae is polygenic. Two QTLs on chromosome 1 were associated with FBO resistance, and several cytochrome P450 monooxygenase (CYP) genes, including CYP392E4, CYP392E6 and CYP392E11, were located near the second QTL. A unique V89A mutation in the subunit c of mitochondrial ATP-synthase, located near the first QTL, was found to be enriched in the resistant parents and selected populations. Marker-assisted introgression experiments demonstrated a significant effect of the V89A mutation on the toxicity of organotin acaricides.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Infectious Diseases
Latifa Elhachimi, Thomas Van Leeuwen, Wannes Dermauw, Carolien Rogiers, Felix Valcarcel, Angeles Sonia Olmeda, Sarah Elhamiani Khatat, Sylvie Daminet, Hamid Sahibi, Luc Duchateau
Summary: In this study, the acaricide susceptibility of Hyalomma marginatum in the Rabat-Sale-Kenitra region of Morocco was investigated. The results showed varying levels of susceptibility to diazinon and amitraz, and emerging resistance to acaricides in ticks.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Biology
Christine Njiru, Wenxin Xue, Sander De Rouck, Juan M. Alba, Merijn R. Kant, Maksymilian Chruszcz, Bartel Vanholme, Wannes Dermauw, Nicky Wybouw, Thomas Van Leeuwen
Summary: The study reveals the presence of a gene family in the two-spotted spider mite that allows it to detoxify plant metabolites, enabling the mite to feed on a wide range of host plants. These genes were acquired through horizontal gene transfer from fungi and are expressed in the mite's gut region. They function as detoxification enzymes by cleaving aromatic rings in plant compounds, preventing them from reaching toxic levels.
Article
Agronomy
Catherine Mermans, Wannes Dermauw, Sven Geibel, Thomas Van Leeuwen
Summary: Afoxolaner demonstrated strong antagonistic activity against GABACls of T. urticae, making it a powerful tool for controlling spider mites. In contrast, other drugs such as fluralaner, fipronil, and endosulfan showed minimal antagonistic activities against GABACls channels of T. urticae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Genetics & Heredity
Andre H. Kurlovs, Berdien De Beer, Meiyuan Ji, Marilou Vandenhole, Tim De Meyer, Rene Feyereisen, Richard M. Clark, Thomas Van Leeuwen
Summary: The study found that in the two-spotted spider mite, trans effects are common in explaining variation in detoxification gene expression, with the striking trans-driven upregulation of a subset of cytochrome P450 monooxygenases of broad substrate specificity in the most pesticide resistant strains.
Article
Biology
Berdien De Beer, Marilou Vandenhole, Christine Njiru, Pieter Spanoghe, Wannes Dermauw, Thomas Van Leeuwen
Summary: The resistance of two-spotted spider mite Tetranychus urticae to bifenthrin is mediated by both target-site mutations and metabolic mechanisms. Increased expression of detoxification enzymes in resistant strains suggests a synergistic effect in conferring resistance.
Article
Evolutionary Biology
Ernesto Villacis-Perez, Wenxin Xue, Marilou Vandenhole, Berdien De Beer, Wannes Dermauw, Thomas Van Leeuwen
Summary: Pesticide resistance in the cosmopolitan pest Tetranychus urticae involves various mechanisms, including target-site insensitivity, metabolic detoxification, and additive or synergistic effects. In this study, researchers used experimental evolution and bulked segregant analysis to map quantitative trait loci (QTL) associated with abamectin resistance in two populations of T. urticae. They found shared QTLs containing genes encoding subunits of the glutamate-gated chloride channel (GluCl), as well as novel candidate loci. The study also revealed a non-functional variant of GluCl2 as a unique resistance mechanism. This research uncovers the complex basis of abamectin resistance and highlights the diversity of genetic mechanisms underlying resistance in this pest.
EVOLUTIONARY APPLICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Wen-Xin Xue, Jing-Tao Sun, Johan Witters, Marilou Vandenhole, Wannes Dermauw, Sabina A. Bajda, Eba A. Simma, Nicky Wybouw, Ernesto Villacis-Perez, Thomas Van Leeuwen
Summary: The two-spotted spider mite Tetranychus urticae has evolved resistance to most classes of pesticides, but different populations show genetic differentiation and reproductive compatibility variation. Understanding these factors is crucial for developing sustainable crop management strategies.
Article
Agronomy
Emre Inak, Berke Demirci, Marilou Vandenhole, Gokhan Soylemezoglu, Thomas Van Leeuwen, Umut Toprak
Summary: Understanding the molecular mechanisms of resistance and cross-resistance between different ketoenol acaricides is crucial to manage resistance. P450 monooxygenases and carboxyl/cholinesterases were found to be involved in resistance. Gene expression analysis confirmed the involvement of P450-mediated resistance, while no target-site mutations were detected.
Review
Biochemistry & Molecular Biology
Sander De Rouck, Emre Inak, Wannes Dermauw, Thomas Van Leeuwen
Summary: The subclass of Acari, known as Arachnida, consists of numerous harmful pests, including spider mites, Varroa (a bee parasite), Dermanyssus (a poultry mite), and various tick species, which pose threats to agriculture and animal health. To minimize the damage caused by these pests, acaricides are frequently used in agriculture, leading to the development of resistance. Additionally, beneficial predatory mites used in biological control are also affected by acaricide selection. The use of new genetic and genomic tools has greatly advanced our understanding of the molecular genetic mechanisms of resistance in Acari, particularly in the model species Tetranychus urticae. These tools have enabled the discovery and validation of new resistance mutations in a wider range of species and have stimulated research on the gene regulation mechanisms associated with detoxification and resistance.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Agronomy
Christine Njiru, Corinna Saalwaechter, Konstantinos Mavridis, John Vontas, Sven Geibel, Nicky Wybouw, Thomas Van Leeuwen
Summary: A target site mutation in a spider mite pest has been found to confer resistance to certain acaricides and has significant fitness costs. These findings highlight the importance of considering multiple factors in assessing the impact of resistance mutations on pest populations.
PEST MANAGEMENT SCIENCE
(2023)