Article
Chemistry, Multidisciplinary
Bon Il Koo, Jae Woong Choi, Seuk Young Song, Ye Hun Choi, Tae Yong Lee, Shin-Hyun Kim, Ki Jun Jeong, Yoon Sung Nam
Summary: Genetically engineered Corynebacterium glutamicum is introduced as a robust cell display platform for the in situ stabilization and low-cost production of carbonic anhydrase (CA). The cell-displayed CA shows high stability at high temperature and provides a promising technique for achieving highly efficient, sustainable, and low-cost CO2 capture for industrial applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Xing Zhu, Chenxi Du, Bo Gao, Bin He
Summary: This study developed a robust bionic jellyfish-carbonic anhydrase complex (Bj-CA) for efficient CO2 capture.
Article
Environmental Sciences
Sefli Sri Wahyu Effendi, Shih- Tan, Wan-Wen Ting, I-Son Ng
Summary: The study explores the use of chaperone GroELS and TrxA-tag to enhance soluble expression of recombinant Sulfurihydrogenibium yellowstonense CA for carbon dioxide conversion. Co-expression of GroELS driven by J23100 promoter led to a significant increase in soluble protein production and CA activity. The involvement of TrxA fusion tag further boosted the soluble protein production and thermostability of SyCA, ultimately improving carbon dioxide conversion efficiency.
Article
Engineering, Chemical
Bin Liu, Zhe Cui, Wende Tian
Summary: Tertiary amines are used as alternative absorbents for carbon capture, but their slow absorption rate increases costs and limits large-scale application. Carbonic anhydrase as a homogeneous catalyst can accelerate CO2 absorption in aqueous tertiary amines, significantly improving the reaction rate.
Article
Biochemistry & Molecular Biology
In Seong Hwang, Joo Hyeon Kim, Byung Hoon Jo
Summary: The study found that the addition of ng3 peptide improved the expression level of NEXT-taCA, while the previously developed TEE and SKIK tags were not effective. It was also discovered that changes in translation initiation rate were not the primary determinant for changes in expression level.
Review
Engineering, Chemical
Hannaneh Rasouli, Kaven Nguyen, Maria C. Iliuta
Summary: Currently, immobilization of the enzyme carbonic anhydrase (CA) is considered a promising approach to enhance the activity, stability, and reusability of CA for CO2 capture. Different immobilization strategies and supports have been developed to improve the performance and efficiency of large-scale applications. This state-of-the-art review analyzes the scientific literature on immobilization of CA and carbon capture technologies using immobilized CA, highlighting the benefits, issues, and limitations. Modelling approaches and techno-economic analyses are also presented to assess the feasibility of enzymatic capture processes in an industrial context.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Environmental
Peijing Shao, Zheng He, Yutao Hu, Yao Shen, Shihan Zhang, Yanan Yu
Summary: Enzyme immobilization using zeolitic imidazolate frameworks, particularly ZIF-8, has been shown to improve the stability and activity levels of carbonic anhydrase (CA) enzymes for flue gas CO2 capture.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Arthur G. G. Fink, Eric W. W. Lees, Julie Gingras, Eric Madore, Sylvie Fradette, Shaffiq A. A. Jaffer, Maxwell Goldman, David J. J. Dvorak, Curtis P. P. Berlinguette
Summary: The electrolysis of carbon capture solutions can convert them into carbon-based products without the need for energy-intensive CO2 recovery steps. Carbonic anhydrase enzymes can enhance CO2 capture by increasing the reaction rate between CO2 and alkaline solutions. A carbon microporous layer can suppress the deactivation of the catalyst caused by carbonic anhydrase.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Maria Elena Russo, Clemente Capasso, Antonio Marzocchella, Piero Salatino
Summary: Carbonic anhydrase (CA) is a promising biocatalyst for the capture and utilization of CO2. Efficient immobilization methods have made it increasingly attractive for continuous bioreactors and industrial applications. This mini-review discusses various CA immobilization techniques and their advantages and disadvantages in CO2 capture and utilization processes. It also highlights the criteria for selecting the optimal CA-biocatalyst and designing CO2 absorption units, as well as presents potential applications of CA in enzymatic CO2 absorption processes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shima Ghaedizadeh, Majid Zeinali, Bahareh Dabirmanesh, Behnam Rasekh, Khosrow Khajeh, Ali Mohammad Banaei-Moghaddam
Summary: Implementing hyperthermostable carbonic anhydrases into CO2 capture and storage technologies can increase the rate of CO2 absorption from industrial flue gases. This study successfully improved the thermostability of a known hyperthermostable carbonic anhydrase through rational engineering of a single-point mutation.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Franziska Steger, Johanna Reich, Werner Fuchs, Simon K. -M. R. Rittmann, Georg M. Guebitz, Doris Ribitsch, Guenther Bochmann
Summary: Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed, and carbonic anhydrases (CAs) can contribute to solving this problem. This current study examines four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab), and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA and PmCA. AwCA showed high stability in a mesophilic temperature range, while PmCA proved to be exceptionally thermostable. These results indicate the potential to utilize CAs from anaerobic microorganisms for CO2 sequestration applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Timothy Wiryaman, Navtej Toor
Summary: Protein nanocompartments are common structures in bacteria and archaea, with functions that are not yet fully understood. The high-resolution cryo-EM structure of a nanocompartment from Thermotoga maritima reveals that interactions in the E-loop domain may be crucial for its thermostability. Additionally, channels at various axes are analyzed for their iron transport capabilities, and an unexpected flavin ligand on the shell suggests a potential direct role in iron metabolism.
Article
Green & Sustainable Science & Technology
Shan Chang, Yan He, Yinxi Li, Xuemin Cui
Summary: This study focused on immobilizing carbonic anhydrases on geopolymer microspheres for CO2 capture and sequestration. Optimal conditions included pH 8.0, temperature 30 degrees C, and incubation for 2 hours. The immobilized enzyme showed improved thermal and storage stability compared to the free enzyme.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Qin Ying, Han Chen, Peijing Shao, Xiaowei Zhou, Xiaojia He, Jiexu Ye, Shihan Zhang, Jianmeng Chen, Lidong Wang
Summary: A core-shell magnetic ZIF-8@Fe3O4-carbonic anhydrase composite was developed to enhance CO2 absorption into tertiary amine, showing a high relative activity. Larger particle size of ZIF-8@Fe3O4 was beneficial for activity but adverse for loading. The developed composite exhibited remarkable magnetic response for convenient recycling.
JOURNAL OF CO2 UTILIZATION
(2021)
Article
Chemistry, Multidisciplinary
Guanzhang Liu, Hang Yuan, Xiaobo Li, Ke Li, Lei Mao, Guangya Zhang
Summary: This study proposes an all-in-one strategy to address the challenges of carbon dioxide capture, conversion, and utilization using carbonic anhydrase (CA) as a green and sustainable method. The strategy involves the self-assembly of nanosized CA oligomeric particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs) with well-designed tags. The prepared mCASPs, with a simple and effective purification method, exhibited high yields and excellent enzymatic properties. Furthermore, the mCASPs could serve as carrier-free immobilized CAs, and the nCAOPs formed from them showed excellent catalytic performances.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Review
Biochemical Research Methods
Sachin Talekar, Byung Hoon Jo, Jonathan S. Dordick, Jungbae Kim
Summary: This article discusses the challenges and solutions in carbonic anhydrase-related technologies and presents the recent advances in utilizing carbonic anhydrase for the production of value-added chemicals through CO2 conversion.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Eunjoo Moon, Eungsu Kang, Wooho Song, Bum Jin Kim, Hyung Joon Cha, Yoo Seong Choi
Summary: Chitosan-based nanofibers are promising biomaterials for wound healing and dressing applications due to their biocompatibility, biodegradability, and antibacterial properties. However, their unstable spinnability has limited their further use. In this study, oleamide, a fatty acid amide, was added to chitosan to improve the spinnability of the nanofibers. The addition of oleamide resulted in lower viscosity of the electrospinning solution, leading to enhanced spinnability. The chitosan/oleamide nanofibers exhibited higher hydrophobicity and decreased tensile strength compared to chitosan-only nanofibers. Cell adhesion in vitro was similar or slightly higher on the blended nanofibers, but cell proliferation was decreased, likely due to the increased hydrophobicity. These findings suggest that chitosan/oleamide nanofibers with improved spinnability could be used for wound dressing with antibacterial properties and moderate hydrophobicity.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Physical
Joo Hyun Kim, Byung Hoon Jo
Summary: A modified Wilbur-Anderson assay using a temperature controllable UV/Vis spectrophotometer and pH indicator phenol red is developed to improve the speed, accuracy, and precision of carbonic anhydrase (CA) activity determination. The results show that the modified assay is a simple and reliable method for routine measurement of CO2 hydration activity.
Article
Biotechnology & Applied Microbiology
Yun Kee Jo, Bong-Hyuk Choi, Cong Zhou, Sang Ho Jun, Hyung Joon Cha
Summary: Titanium mesh for guided bone regeneration (GBR) has been widely used due to its mechanical properties and biocompatibility. However, soft tissue invasion and limited bioactivity often affect the outcomes of GBR treatments. In this study, a cell recognitive osteogenic barrier coating using a bioengineered mussel adhesive protein fused with Alg-Gly-Asp peptide was developed. The coating showed excellent performance in promoting bone regeneration in vitro and in vivo, making it a promising therapeutic platform for GBR treatment.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jaeyun Lee, Ha Neui Choi, Hyung Joon Cha, Yun Jung Yang
Summary: Diverse forms and materials of hemostatic agents are necessary for controlling excessive bleeding during surgery. The use of these agents can significantly reduce the risk of dehydration, lack of oxygen, and even death. Polysaccharide-based agents, such as starch, are widely used due to their safety. In this study, starch was blended with silk protein and crosslinked to improve its structural integrity. The resulting sponge-like material exhibited increased swelling ability and underwater retentivity, promoting blood coagulation. Animal bleeding model tests confirmed the clinical effectiveness of these sponges as topical hemostatic agents.
Article
Biochemistry & Molecular Biology
Wonchan Park, Seong-Woo Maeng, Jee Won Mok, Minji Choi, Hyung Joon Cha, Choun-Ki Joo, Sei Kwang Hahn
Summary: A facile method for early diagnosis of tumors is developed by extracting exosomes from interstitial fluid using hydrogel microneedles. The hydrogel MNs expand in the skin to absorb the ISF, and the tumor exosomes bind with the glypican-1 antibodies inside the hydrogel. Exosomes are separately purified from the ISF to analyze tumor-related biomarkers, and colorectal cancer can be diagnosed by ELISA for model mice. This noninvasive hydrogel MN system plays an important role in early cancer diagnosis.
Article
Biotechnology & Applied Microbiology
Junghwan Heo, Eun Young Jeon, Kye Il Joo, Hyung Joon Cha
Summary: In this study, a self-biodegradable tissue adhesive micro-needle (MN) patch was developed for localized and sustained delivery of doxorubicin (DOX) via microchannels, leading to effective inhibition of melanoma growth.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2023)
Article
Engineering, Environmental
Seong-Woo Maeng, Ji-Yun Ko, Tae Yoon Park, Jinyoung Yun, So Hyun Park, Sang Jun Han, Kye Il Joo, Seongmin Ha, Mingoo Jee, Gun-Il Im, Hyung Joon Cha
Summary: Articular cartilage (AC), a smooth tissue that covers bones and absorbs impacts, can be damaged and trigger osteoarthritis (OA). Cell transplantation has been explored for AC reconstitution, but the lack of cell retention has limited its efficacy. This study developed a bioadhesive using mussel adhesion protein (MAP) and high-molecular-weight hyaluronic acid (HA) to encapsulate and retain transplanted stem cells in defective AC. The results showed that the MAP-HA complex coacervate improved cell survival and therapeutic effects in the treatment of OA.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Ae Sol Lee, Su Min Kim, Kyeong Rok Kim, Chulmin Park, Dong-Gun Lee, Hyung Joon Cha, Hye Ryoung Heo, Chang Sup Kim
Summary: To control SARS-CoV-2, early diagnosis of infected individuals is crucial. A colorimetric lateral flow immunoassay (LFIA) was developed in this study, enabling sensitive detection of cultured SARS-CoV-2 in 15 minutes with a detection limit of 5 x 10^4 copies/mL. Clinical evaluation showed that the LFIA accurately detected all positive and negative samples, achieving 100% accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hyun Sun Choi, Ji Hye Yum, Yeonsu Jeong, Soobin Lim, Tomotaka Kumagai, Hyung Joon Cha, Soyoung Park
Summary: Triple negative breast cancer (TNBC) affects young women's health and has a socioeconomic impact. Conventional diagnostic methods have difficulties in detecting TNBC. A fluorescent probe is described here that allows rapid and sensitive visualization of TNBC cells, with potential for extensive therapeutic applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Applied
Sangmin Lee, Geunho Choi, Yun Jung Yang, Kye Il Joo, Hyung Joon Cha
Summary: Researchers developed a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains. This photocrosslinkable alginate microgel showed higher cell viability and printing resolution compared to the bulk gel form, and demonstrated excellent 3D bioprinting ability and structural stability. It provided an effective strategy for constructing implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.
CARBOHYDRATE POLYMERS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Jaeyun Lee, Eun Jin Kim, Ki Joo Kim, Jong Won Rhie, Kye Il Joo, Hyung Joon Cha
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Hyun Sun Choi, Eunseo Kim, Hyomin Lee, Hyung Joon Cha
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Hyun Tack Woo, Hyung Joon Cha
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Jinyoung Yun, Hyung Joon Cha
TISSUE ENGINEERING PART A
(2022)
