期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 91, 期 -, 页码 52-58出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2013.02.009
关键词
Yeast cells; Invertase; Immobilization; Electropolymerization; Conducting polymers
资金
- Scientific and Technological Research Council of Turkey (TUBITAK) [109T439]
- Scientific Research Projects Foundation of Karamanoglu Mehmetbey University (KMU-BAP) [09-M-11]
- Scientific and Technological Research Council of Turkey [TUBITAK-2209]
Yeast cells (Saccharomyces cerevisiae) and invertase enzyme were immobilized in thiophene capped poly(ethyleneoxide)/polypyrrole (PEO-co-PPy) and 3-methylthienyl methacrylate-co-p-vinyl benzyloxy poly(ethyleneoxide)/polypyrrole (CP-co-PPy) matrices. Immobilization of the enzyme and yeast cells was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole through the thiophene moiety of the polymers. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values, operational and storage stabilities of the enzyme and yeast cell electrodes were investigated. (c) 2013 Elsevier B.V. All rights reserved.
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