Biocatalytic activity of immobilized pointed gourd (Trichosanthes dioica) peroxidase-concanavalin A complex on calcium alginate pectin gel

Salt fractionated pointed gourd (Trichosanthes dioica) peroxidase-concanavalin A (PGP-Con A) complex expressed 79% of original peroxidase activity which decreased on entrapment into calcium alginate-pectin gel. Immobilized PGP-Con A complex retained 82.7% activity even at 60 C which was achieved at pH 4.0. Urea treatment resulted in activity loss by similar to 40%. With dioxane, immobilized PGP exhibited an activity of over 55% whereas an increasing concentration of dimethylformide resulted in decline. Activity of immobilized PGP-Con A was dependent on nature and concentration of detergent. Michaelis-Menten constant (K-m) and V-max for entrapped PGP-Con A complex was 0.08 mM and 15.7 mM/min. respectively. This study shows the efficacy, durability and sustainability of immobilized catalytic system which could be efficiently used for the removal of synthetic dyes from industrial effluents. (C) 2011 Elsevier B.V. All rights reserved.