期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 68, 期 2, 页码 206-210出版社
ELSEVIER
DOI: 10.1016/j.molcatb.2010.11.005
关键词
Direct electrochemistry; Carbon nanotube; TiO2; Glucose oxidase
资金
- Research Councils of Sharif University of Technology
- University of Tehran
A nano-composite material consisting of amine functionalized TiO2-coated carbon nanotubes was prepared and used for glucose oxidase (GOx) absorption. The GOx bearing nanomaterial was fixed on a glassy carbon electrode to construct a novel biosensor for glucose determination. The direct electrochemistry of immobilized GOx and its electron transfer parameters at the modified glassy carbon electrode were reported. The apparent heterogeneous electron transfer rate constant (k(s)) of GOx was estimated to be 3.5 s(-1), which is higher than those reported previously. Amperometric detection of glucose resulted in a rapid (3 s) and stable response in the linear concentration range from 1.8 to 266 mu M. The apparent Michaelis-Menten constant was measured to be 8.59 mM, indicating that the immobilized GOx on NH2-TiO2-CNT matrix retained its native activity. The anti-interference ability and long-term stability of the biosensor were also assessed. (c) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据