期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 69, 期 1-2, 页码 1-7出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2010.12.002
关键词
Biosensor; Prussian blue-gold nanocomposite; Platinum nanoclusters; Glucose oxidase; Electrochemical deposition
资金
- National Natural Science Foundation of China [20675064]
- Key Lab of Chongqing Modern Analytical Chemistry
- Doctor Foundation of Southwest University [SWUB2008048]
- Fundamental Research Funds for the Central Universities [XDJK2009B013, XDJK2009C082]
A glucose biosensor was developed, which was based on Prussian blue-gold (PB-Au) nanocomposite films and platinum nanoclusters (Pt-NCs). Prussian blue (PB), as an electron mediator, was electrochemically deposited on the glass carbon electrode (GCE) in the presence of chloroauric acid, forming PB-Au nanocomposite films. Then. Pt-NCs were electrodeposited to construct a bilayer film. At last, glucose oxidase was modified on the electrode with the bilayer film and the film of Nafion (Nf) was used to prevent the glucose oxidase (GOD) from leaking off. The resulting amperometric glucose biosensor exhibited a fast response time (within 8 s) and a linear calibration range from 3.0 mu M to 1.1 mM with a low detection limit of 1.0 mu M glucose (S/N = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including acetum acid, uric acid and arginine, indicating an excellent selectivity. The improved performances of resulting electrode for electrocatalysis-oxidation glucose were ascribed to the high surface-to-volume ratio and the excellent catalytic activity of nanoparticles. The combination of the PB-Au nanocomposite and Pt-NCs would open new horizons for fabrication of biosensors and biocatalysts. (C) 2010 Elsevier B.V. All rights reserved.
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