Review
Immunology
Julia Hambach, Anna Marei Mann, Peter Bannas, Friedrich Koch-Nolte
Summary: This article discusses the specificity and effectiveness of nanobody-based constructs in targeting CD38-expressing myeloma cells. Promising results have been obtained in preclinical studies, warranting further clinical research to evaluate the potential of these CD38-specific nanobody-based constructs for the treatment of multiple myeloma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Patrycja Sroga, Angela Sloan, Bryce M. Warner, Kevin Tierney, Jocelyne Lew, Guodong Liu, Michael Chan, Yvon Deschambault, Derek R. Stein, Geoff Soule, Logan Banadyga, Darryl Falzarano, David Safronetz
Summary: The study demonstrates the potential of utilizing unique camelid-based IgG antibodies for prevention and treatment of deadly viral pathogens, showing reduction in viral burderns and organ-specific pathology associated with lethal diseases like HPS. Further pre-clinical evaluation is warranted due to the promising results.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Yi Gu, Yang Guo, Yang Deng, Haipeng Song, Rui Nian, Wenshuai Liu
Summary: A novel pentameric nanobodies-based immunoassay (PNIA) demonstrated superior sensitivity and high specificity for detecting CEACAM-5 antigen. The use of bispecific or multivalent nanobody design provided new insights into the development of immunoassays for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Multidisciplinary
Bernadette Tse Sum Bui, Alejandra Mier, Karsten Haupt
Summary: Molecularly imprinted polymers (MIPs) are synthetic polymers that mimic antibodies by nanomoulding the shape and chemical functionalities of a target molecule. They show exquisite molecular recognition ability and have potential applications in diagnostics and therapeutics, replacing antibodies. This review focuses on the development of MIPs targeting proteins, from their synthesis in bulk format to soluble nanogels, with emphasis on epitope imprinting for selective recognition of macromolecules.
Article
Immunology
Mukhtar Ahmad Dar, Pawan Kumar, Prakash Kumar, Ashish Shrivastava, Muneer Ahmad Dar, Richa Chauhan, Vinita Trivedi, Ashutosh Singh, Eshan Khan, Ravichandiran Velayutham, Sameer Dhingra
Summary: This study designed a peptide-based multi-epitope vaccine construct against gallbladder cancer using immunoinformatics approaches. The designed vaccine construct showed satisfactory validation of the secondary and tertiary models, and demonstrated good binding affinity with TLR 2, 3, and 4 receptors.
Article
Chemistry, Analytical
Zilong Liu, Xing Liu, Hua Pei, Kunlu Bao, Benchao Su, Hongmei Cao, Long Wu, Qi Chen
Summary: We developed a convenient method for the fabrication of homogeneous multivalent primary antibody-enzyme complexes (PAECs) using protein self-assembly and validated it using anti-alpha-fetoprotein nanobody and alkaline phosphatase as models. The heptavalent PAECs showed a 4-fold enhancement in enzymatic catalytic activity compared to monovalent PAECs. The developed heptavalent PAEC-based ELISA demonstrated a detection limit of 0.69 ng mL(-1), about 3 times higher than that of monovalent PAECs, with the entire detection process completed within 3 hours.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Lijie Ren, Zhigang Zhang, Xinyuan Zhang, Zhibo Kong, Shaowei Li, Qinjian Zhao
Summary: By utilizing a fluorescence imaging-based high content analysis, this study analyzed the interactions of protein molecules on particle surfaces, demonstrating the feasibility of analyzing subtle conformational changes in antigen epitopes. The method is highly amenable for automation and is an ideal choice for formulation development and stability testing of products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Agriculture, Multidisciplinary
Jinqiang Hu, Luyang Yuan, Guangjie An, Junsong Zhang, Xuewei Zhao, Yang Liu, Jinjin Shan, Zhangcun Wang
Summary: This study analyzed the changes in molecular composition and antigenicity of soybean beta-conglycinin hydrolyzed by pepsin, and identified the presence of complete sequential epitopes in the resulting hydrolysates as a reason for the incomplete disappearance of antigenic activity. The peptides generated from enzymatic hydrolysis showed some resistance to further hydrolysis, suggesting a potential mechanism for the persistence of antigenic activity in beta-conglycinin.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Food Science & Technology
Jinqiang Hu, Yang Liu, Guangjie An, Junsong Zhang, Xuewei Zhao, Peiqi Zhang, Zhangcun Wang
Summary: The study found that beta-conglycinin is difficult to completely eliminate its antigenicity through enzymatic hydrolysis due to its evident resistance to digestion. Even though the protein was partially degraded, it still retained antigenic activity, suggesting a relationship between the existence of anti-enzymolysis fragments and incomplete elimination of antigenicity.
Article
Biochemistry & Molecular Biology
Yiran Zhang, Shihao Cheng, Peiyu Zhong, Ziying Wang, Rui Liu, Yu Ding
Summary: The study identified nanobody Rh57 that specifically binds to active RhoA-GTP, providing a potential for developing a non-cytotoxic BRET biosensor. Structural analysis revealed the interaction mechanism between Rh57 and RhoA, offering critical information for optimizing relevant nanobodies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Yucheng Guo, Zhenlin Ouyang, Wenbo He, Jiaxin Zhang, Qian Qin, Min Jiao, Serge Muyldermans, Fang Zheng, Yurong Wen
Summary: This study focuses on the screening and structural characterization of nanobodies targeting full length and active BFT. The results provide a new strategy for the early diagnosis of ETBF and the possibility for BFT as a biomarker for diagnosing diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Farrukh Jamil, Laiba Aslam, Laraib, Hira Ali, Kainaat Shoukat, Muhammad Asif Rasheed, Sohail Raza, Muhammad Ibrahim
Summary: A new multi-epitope vaccine with higher efficiency against Newcastle disease virus (NDV) has been designed by shuffling cytotoxic T lymphocytes segments. This new vaccine construct shows improved immunogenicity, antigenicity, and stability, making it more effective against NDV strains in Pakistan.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Paul Stahl, Sebastian Kollenda, Jonas Sager, Laura Schmidt, Martin A. Schroer, Roland H. Stauber, Matthias Epple, Shirley K. Knauer
Summary: A nanobody (SuN) targeting the disease-relevant protein Survivin is used to track its concentration inside cells but fails to inhibit its biological functions. The coupling of multiple SuN to ultra-small gold nanoparticles (SuN-N) enables intracellular uptake and interference with mitotic progression. This demonstrates the universal applicability of coupling nanobodies to nanosized scaffolds to improve their function and therapeutic applicability.
Article
Immunology
Fang Zheng, Jinhong Zhou, Zhenlin Ouyang, Jiaxin Zhang, Xinyi Wang, Serge Muyldermans, Jo Van Ginderachter, Nick Devoogdt, Yurong Wen, Steve Schoonooghe, Geert Raes
Summary: Nanobodies derived from camelids have been developed targeting the Kupffer cell-specific receptor Clec4F, with a strong affinity and the ability to specifically target the liver. Structural characterization revealed distinct features within the CDR2 and CDR3 regions of the nanobodies, indicating different recognition epitopes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuexing Liu, Li Yi, Yixue Li, Zhen Wang, Jirimutu
Summary: Camelids are the only mammals capable of producing functional heavy-chain antibodies. This study explores the antibody gene repertoire of Bactrian camels and reveals their similarities and differences with other camelid species. It provides insights into the diversity and expression of VHHs and adds useful resources for HCAb engineering.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Article
Instruments & Instrumentation
Junos Lukan, Klemen Ambrozic, Gasper Zerovnik, Luka Snoj
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
(2017)
Article
Multidisciplinary Sciences
Ariel Talavera, Jelle Hendrix, Wim Versees, Dukas Jurenas, Katleen Van Nerom, Niels Vandenberk, Ranjan Kumar Singh, Albert Konijnenberg, Steven De Gieter, Daniel Castro-Roa, Anders Barth, Henri De Greve, Frank Sobott, Johan Hofkens, Nikolay Zenkin, Remy Loris, Abel Garcia-Pino
Article
Biochemistry & Molecular Biology
Peter D. Szigetvari, Gopinath Muruganandam, Juha P. Kallio, Erik Hallin, Agnete Fossbakk, Remy Loris, Inari Kursula, Lisbeth B. Moller, Per M. Knappskog, Petri Kursula, Jan Haavik
JOURNAL OF NEUROCHEMISTRY
(2019)
Article
Biophysics
Pieter De Bruyn, San Hadzi, Alexandra Vandervelde, Albert Konijnenberg, Marusa Prolic-Kalinsek, Yann G-J Sterckx, Frank Sobott, Jurij Lah, Laurence Van Melderen, Remy Loris
BIOPHYSICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Ariel Talavera, Hedvig Tamman, Andres Ainelo, Albert Konijnenberg, San Hadzi, Frank Sobott, Abel Garcia-Pino, Rita Horak, Remy Loris
NATURE COMMUNICATIONS
(2019)
Article
Biophysics
Marusa Prolic-Kalinsek, Pieter De Bruyn, Dukas Jurenas, Laurence Van Melderen, Remy Loris, Alexander N. Volkov
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biochemical Research Methods
Gabriela Garcia-Rodriguez, Ariel Talavera Perez, Albert Konijnenberg, Frank Sobott, Jan Michiels, Remy Loris
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2020)
Article
Environmental Sciences
Larissa Bolliger, Junos Lukan, Elena Colman, Leen Boersma, Mitja Lustrek, Dirk De Bacquer, Els Clays
Summary: This study used a qualitative research approach to identify work stressors as perceived by office workers. The findings confirmed existing data from occupational stress literature and highlighted the multi-level complexity of work stress experiences. The study also revealed the presence of relevant stressors experienced by office workers that go beyond existing quantitative frameworks.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Public, Environmental & Occupational Health
Larissa Bolliger, Gillian Debra, Junos Lukan, Rani Peeters, Elena Colman, Ellen Baele, Mitja Lustrek, Dirk De Bacquer, Els Clays
Summary: This study investigated the relationships between day-to-day job demands, job control, job strain, social support at work, and work-life interference among office workers in academia. The findings showed that higher job demands and job strain were associated with higher work interfering with personal life, while higher job control was associated with lower personal life interfering with work.
INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH
(2023)
Article
Multidisciplinary Sciences
Larissa Bolliger, Ellen Baele, Elena Colman, Gillian Debra, Junos Lukan, Mitja Lustrek, Dirk De Bacquer, Els Clays
Summary: This study examined the associations between day-to-day work-related stress exposures, job strain, and next-day work engagement among office workers in academic settings. It also investigated the influence of psychological detachment and relaxation on next-day work engagement, as well as the interaction effects of these recovery variables.
Article
Multidisciplinary Sciences
Uros Zavrtanik, San Hadzi
Article
Chemistry, Multidisciplinary
San Hadzi, Andrej Mernik, Crtomir Podlipnik, Remy Loris, Jurij Lah
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2017)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)