Review
Genetics & Heredity
Michael Cargill, Rasika Venkataraman, Stanley Lee
Summary: DEAD-box RNA helicases are crucial regulators of RNA metabolism and are linked to genome stability and cancer development. They play important roles by regulating the expression of factors related to genome stability and repairing DNA damage.
Article
Multidisciplinary Sciences
Qi Li, Ningkun Liu, Qing Liu, Xingguo Zheng, Lu Lu, Wenrui Gao, Yang Liu, Yan Liu, Shicheng Zhang, Qian Wang, Jing Pan, Chen Chen, Yingjie Mi, Meiling Yang, Xiaofei Cheng, Guodong Ren, Yao-Wu Yuan, Xiaoming Zhang
Summary: The RNA helicases RH6, RH8, and RH12 play key roles in modulating the formation of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis via liquid-liquid phase separations (LLPSs). Their accumulation decreases in the nuclei upon Turnip mosaic virus infections, leading to a decrease in D-bodies.
Review
Cell Biology
Lu Zhang, Xiaogang Li
Summary: RNA helicases play essential roles in regulating the cell cycle by influencing various cellular processes such as cell proliferation, cell cycle arrest, and apoptosis, impacting multiple diseases. Studies have shown their involvement in regulating progression through different phases of the cell cycle.
Review
Oncology
Massimiliano Secchi, Camilla Lodola, Anna Garbelli, Silvia Bione, Giovanni Maga
Summary: The transformation of a normal cell into a cancerous one is caused by the deregulation of metabolic pathways and protein-protein interactions. Cellular enzymes DDX3X and DDX5 play important roles in normal cell metabolism, but their deregulation can accelerate tumor transformation. This review summarizes the roles of DDX3X and DDX5 in different tumors and discusses their protein-protein interactions in cancer proliferation.
Article
Biochemistry & Molecular Biology
Yongliang Wang, Junwen Liao, Jun Wu, Huimei Huang, Zhanxin Yuan, Wei Yang, Xinying Wu, Xia Li
Summary: In this study, we identified 80 DEAD-box genes in the soybean genome and found that most of these genes were highly conserved in Arabidopsis. However, two genes were specific to soybean. The promoters of these genes contained cis-acting elements involved in plant responses to various stresses, and half of the genes contained nodulation-related elements. Microarray analysis showed differential expression of the DEAD-box genes in roots and nodules, with 31 genes being induced or highly expressed in nodules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai
Summary: RNA helicases, such as Prp16 and Prp22, promote correct mRNA synthesis by binding and proofreading on their RNA substrates in defined spliceosome complexes. The stability of spliceosome during exon ligation may affect the proofreading activity of helicases. The new method psiCLIP provides key insights into the binding and proofreading activity of spliceosomal RNA helicases.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Bichun Zheng, Xudong Chen, Qiaoyun Ling, Quan Cheng, Shaoshun Ye
Summary: This review summarizes the role of DEAD-box RNA helicase proteins in CRC and discusses their cellular mechanisms, aiming to provide new strategies for the treatment of CRC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Infectious Diseases
Ashaq Hussain, Malay Kumar Ray
Summary: This study demonstrates the protective role of DEAD box RNA helicases against nucleic acid damaging agents. The results show that different mutants exhibit varying sensitivities to different DNA damaging agents.
INFECTION GENETICS AND EVOLUTION
(2022)
Review
Immunology
Hongjuan You, Lihong Ma, Xing Wang, Fulong Zhang, Yiran Han, Jiaqi Yao, Xiucheng Pan, Kuiyang Zheng, Fanyun Kong, Renxian Tang
Summary: DEAD/H-box helicases are essential proteins that regulate RNA metabolism and innate immune responses. They have been implicated in the development of various diseases, particularly those related to HBV infection. Recent studies have shown that DEAD/H-box helicases interact with HBV, modulating viral replication and innate signaling pathways. Targeting these helicases may be a promising treatment strategy for HBV infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Cell Biology
Shringar Rao, Tokameh Mahmoudi
Summary: This review provides an overview of the functions and mechanisms of DEAD-box family proteins in HIV-1 vRNA metabolism. It discusses the implications of these interactions in viral infection, viral latency, and cell death, and speculates on the potential of DEAD-box protein inhibitors as therapeutics for controlling the HIV-1 pandemic.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Qing Feng, Keegan Krick, Jennifer Chu, Christopher B. Burge
Summary: This study reveals a widespread splicing quality control function for DHX15 in human cells and identifies SUGP1 as a factor that activates DHX15's splicing QC function.
Review
Biochemistry & Molecular Biology
Joon Tae Park, Sekyung Oh
Summary: RNA helicase DDX3 is an important regulator of translation and can remodel complex RNA structures by unwinding RNA molecules. It interacts with specific RNA molecules and ribosomal RNA to facilitate or repress translation. Recent studies have revealed that DDX3 plays critical roles in various modes of translation and disease-associated DDX3 variants can alter the translation landscape in cells.
Article
Biochemistry & Molecular Biology
Sarah Venus, Kaba Tandjigora, Eckhard Jankowsky
Summary: The protein K7 from vaccinia virus is shown to inhibit the biochemical activities and cellular functions of DDX3X by binding to its N-terminus, restraining its association with stress granules.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Chi Xu, Zhanhui Zhang, Juan He, Yongsheng Bai, Jie Cui, Lin Liu, Jihua Tang, Guiliang Tang, Xuemei Chen, Beixin Mo
Summary: RCF1 is shown to play a role in pri-miRNA processing and splicing, as well as pre-mRNA splicing in Arabidopsis thaliana. A mutant with miRNA biogenesis defects was identified, and it was found to be caused by a recessive point mutation in RCF1 (rcf1-4). RCF1 promotes D-body formation and facilitates the interaction between pri-miRNAs and HYL1, and rcf1-4 exhibits a global splicing defect in intron-containing pri-miRNAs and pre-mRNAs. This study uncovers the importance of RCF1 in miRNA biogenesis and RNA splicing in Arabidopsis.
Review
Biochemistry & Molecular Biology
Ali Khreiss, Katherine E. Bohnsack, Markus T. Bohnsack
Summary: During the biogenesis process of ribosomal subunits, RNA helicases play a crucial role in remodeling events through structural and compositional changes. However, understanding their specific functions has been challenging due to limited knowledge on their molecular functions and RNA substrates. Recent advances in RNA helicase characterization, identification of their binding sites on pre-ribosomes, and structural analysis of pre-ribosomal complexes containing RNA helicases provide new insights into how different RNA helicases contribute to ribosomal subunit maturation.
BIOLOGICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Xuan Ye, Eckhard Jankowsky
Review
Cell Biology
Donny D. Licatalosi, Xuan Ye, Eckhard Jankowsky
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2020)
Article
Biochemistry & Molecular Biology
Sukanya Srinivasan, Zhonghua Liu, Watchalee Chuenchor, Tsan Sam Xiao, Eckhard Jankowsky
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Correction
Biochemistry & Molecular Biology
Andrea Putnam, Madeline Cassani, Jarrett Smith, Geraldine Seydoux
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Armend Axhemi, Elizabeth V. Wasmuth, Christopher D. Lima, Eckhard Jankowsky
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Sai Kiran Naineni, Rayelle Itoua Maiga, Regina Cencic, Andrea A. Putnam, Luis A. Amador, Abimael D. Rodriguez, Eckhard Jankowsky, Jerry Pelletier
Article
Biology
Chih-Yung S. Lee, Andrea Putnam, Tu Lu, ShuaiXin He, John Paul T. Ouyang, Geraldine Seydoux
Article
Biochemistry & Molecular Biology
Beth Walters, Armend Axhemi, Eckhard Jankowsky, Sunnie R. Thompson
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Xuan Ye, Armend Axhemi, Eckhard Jankowsky
Summary: The 3' to 5' exonuclease Pop2p is part of the CCR4-NOT deadenylation complex, with structurally conserved but functionally unusual enzymatic properties in Saccharomyces cerevisiae. The enzyme degrades RNAs via two alternative reaction pathways, with three distinct reaction phases, and demonstrates functional redundancy of amino acids in the catalytic core. Mutations in the catalytic center moderately reduce enzymatic activity, while complete mutation of all five amino acids is required to diminish activity to background levels.
Article
Cell Biology
Haidong Huang, Xingjiang Yu, Xiangzi Han, Jing Hao, Jianjun Zhao, Gurkan Bebek, Shideng Bao, Richard A. Prayson, Ahmad M. Khalil, Eckhard Jankowsky, Jennifer S. Yu
Summary: The Piwi-like family members are overexpressed in glioblastoma, with Piwil1 being the most frequently overexpressed. Piwil1 is enriched in glioma stem-like cells to maintain self-renewal, and its knockdown leads to changes in gene expression and cell cycle arrest, senescence, or apoptosis. Piwil1 regulates the mRNA stability of key genes involved in cancer stem cell maintenance, and its knockdown suppresses tumor growth and promotes survival in animal models of glioblastoma.
Article
Multidisciplinary Sciences
Deepak Sharma, Leah L. Zagore, Matthew M. Brister, Xuan Ye, Carlos E. Crespo-Hernandez, Donny D. Licatalosi, Eckhard Jankowsky
Summary: The study using pulsed femtosecond ultraviolet laser for time-resolved RNA-protein cross-linking reveals the binding and dissociation kinetics of RBP DAZL in cells. The results show that DAZL resides at individual binding sites for only seconds, while the sites remain DAZL-free for longer periods, and it binds to many RNAs in clusters of multiple proximal sites.
Article
Biochemistry & Molecular Biology
Mengyuan Xu, Armend Axhemi, Magdalena Malgowska, Yinghua Chen, Daniel Leonard, Sukanya Srinivasan, Eckhard Jankowsky, Derek J. Taylor
Summary: The POT1-TPP1 complex interacts specifically with telomere DNA, destabilizing G4 DNA in two modes depending on protein concentration; cancer-associated POT1-TPP1 mutations impair efficient destabilization of G4 structures.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrew W. Folkmann, Andrea Putnam, Chiu Fan Lee, Geraldine Seydoux
Summary: This study reveals that the dynamics of biomolecular condensates are regulated by protein clusters. Through experiments and theory, it is demonstrated that the assembly of P granules is controlled by MEG-3 protein, which helps lower surface tension and slow down coarsening.
Article
Developmental Biology
Laura Thomas, Andrea Putnam, Andrew Folkmann
Summary: A characteristic of germ cells is the presence of germ granules, which are assemblies of proteins and RNA lacking a membrane and formed through condensation. Germ granules are conserved across organisms and developmental stages, and are believed to be involved in germ cell development. However, the molecular functions of germ granules are still not fully understood. In this Development at a Glance article, the authors survey germ granules in different organisms and developmental stages, highlighting emerging themes regarding granule regulation, dynamics, and proposed functions.
Article
Multidisciplinary Sciences
W. Michael Babinchak, Benjamin K. Dumm, Sarah Venus, Solomiia Boyko, Andrea A. Putnam, Eckhard Jankowsky, Witold K. Surewicz
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)
