Article
Biochemistry & Molecular Biology
Frank Burmann, Louise F. H. Funke, Jason W. Chin, Jan Lowe
Summary: The ring-like SMC complex MukBEF is able to fold the genome of bacteria into large loops, and its activity in the replication terminus macrodomain is suppressed by the sequence-specific unloader MatP. The complete atomic structure of MukBEF in complex with MatP and DNA has been determined, revealing its interaction with the bacterial chromosome and the entrapment of DNA loops.
Article
Multidisciplinary Sciences
Victoria C. Young, Pablo Artigas
Summary: Utilizing state-specific engineered-disulfide cross-linking, it was demonstrated that the transmembrane segment 2 (M2) of the Na+/K+ pump moves in directions inconsistent with distances observed in existing crystal structures of the pump. This movement was characterized using voltage-clamp fluorometry in single-cysteine mutants, revealing state-dependent fluorescence changes upon labeling with tetramethylrhodamine-6-maleimide (TMRM).
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Environmental Sciences
Lijin An, Xiong Luo, Minghan Wu, Liling Feng, Kaixiang Shi, Gejiao Wang, Barry P. Rosen, Mingshun Li
Summary: The study reveals a novel bacterial P-type ATPase playing a crucial role in antimony detoxification, with unique structure and function compared to other members. AntC acts as an Sb(III) chaperone facilitating Sb(III) efflux, enhancing resistance to Sb(III).
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Oncology
Eugenia M. Yazlovitskaya, Todd R. Graham
Summary: Lipids are crucial for cellular functions and play a significant role in cancer development and progression. Type IV P-type ATPases, known as flippases, regulate membrane lipid asymmetry and are closely associated with cancer-related lipid changes. Recent studies have focused on the role of flippases, particularly P4-ATPases, in cancer and their potential as therapeutic targets.
Article
Chemistry, Medicinal
Gangqiang Yang, Shuqi Liu, Chen Zhang, Liping Yu, Zongji Zou, Conghui Wang, Meng Gao, Shuang Li, Yiqi Ma, Ruoxuan Xu, Zhihua Song, Rongxia Liu, Hongbo Wang
Summary: In this study, nonsubstrate allosteric inhibitors of P-glycoprotein (Pgp) were designed and synthesized. A potential nonsubstrate inhibitor (7a) was found to bind to the allosteric site of Pgp with high affinity and inhibit its ATPase activity. It also interfered with Pgp-mediated efflux and enhanced the therapeutic efficacy of paclitaxel in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Qiaojing Huang, Pengbo Song, Yixin Chen, Zhirong Liu, Luhua Lai
Summary: The study investigated protein allostery and developed a method for predicting the type and pathways of allostery, successfully predicting the types and pathways of multiple allosteric systems. The AlloType method demonstrated potential applications in designing specific allosteric drugs and uncovering allosteric mechanisms.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Srihari Shankar, Sarath Ramachandran, Nikhil Tulsian, Sridhar Radhakrishnan, Chacko Jobichen, J. Sivaraman
Summary: Glutaminase is a metabolic enzyme that plays a role in cancer cell proliferation. A new allosteric site has been identified in the enzyme, which can be targeted to develop specific inhibitors for the treatment of cancer.
Article
Biology
Raymond N. Burton-Smith, Chihong Song, Hiroshi Ueno, Takeshi Murata, Ryota Iino, Kazuyoshi Murata
Summary: Using cryo-electron microscopy, we have captured six intermediate conformations of the active rotary vacuolar-type ATPase from Enterococcus hirae, revealing a non-uniform rotor rotation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weixue Wang, Laurence Mevellec, Annie Liu, Geoff Struble, Robyn Miller, Samantha J. Allen, Kelly Federowicz, Berthold Wroblowski, Jorge Vialard, Kay Ahn, Daniel Krosky
Summary: Targeting inactive kinase conformations can lead to more selective kinase inhibitors. By applying cascade assays, a highly selective inhibitor for the cancer immunotherapy target kinase HPK1 was discovered, indicating the potential for further development of potent and selective inhibitors.
Article
Biochemistry & Molecular Biology
Yugo Miyata, Kyoko Yamada, Shigekazu Nagata, Katsumori Segawa
Summary: Phospholipids in plasma membranes are asymmetrically distributed, and ATP11A and ATP11C can flip PtdSer to the inner leaflet. Introducing the functions of ATP11A and ATP11C can restore PtdSer asymmetry in ATP11A(-/-) ATP11C(-/-) cells. ATP8A1 and ATP11B achieve this function through endocytosis at 37℃.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
An Duan, Ziheng Wang, Xiaoyu Huang, Yongjun Li
Summary: Metal-free organic electrode materials have been widely studied due to their designable structures and tunable electrochemical properties. This article introduces a new p-type polymeric cathode material, poly(2-vinyl-5,10-dimethyl-dihydrophenazine) (PVDMP), with a theoretical capacity of 227 mAh g(-1). The study investigates the electrochemical behavior of PVDMP-based cathode, which depends on the doping of two anions to maintain electroneutrality during oxidation. The optimal conditions for PVDMP cathode are identified, resulting in high initial capacity and excellent cycling stability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Mathematics, Applied
Roberto Corcino, Mary Ann Ritzell Vega, Amerah Dibagulun
Summary: In this paper, the type 2 (p,q)-analogue of the r-Whitney numbers of the second kind is defined, along with a combinatorial interpretation in the context of A-tableaux. Convolution-type identities useful for deriving the Hankel transform of the type 2 (p,q)-analogue of the r-Whitney numbers of the second kind are obtained. Lastly, the Hankel transform of the type 2 (p,q)-analogue of the r-Dowling numbers is established.
Article
Multidisciplinary Sciences
Rizaldy C. Zapata, Maria Carretero, Felipe Castellani Gomes Reis, Besma S. Chaudry, Jachelle Ofrecio, Dinghong Zhang, Roman Sasik, Theodore Ciaraldi, Michael Petrascheck, Olivia Osborn
Summary: Energy metabolism remains dysregulated after weight loss in individuals with obesity, known as "metabolic memory". Using mouse and C. elegans models, this study identified ATP6v0a1 as a key gene involved in regulating food intake and weight gain, persisting even after weight loss.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Dmitrii A. Matalin, Dmitrii E. Khramov, Alexey V. Shuvalov, Vadim S. Volkov, Yurii V. Balnokin, Larissa G. Popova
Summary: The microalga genus Dunaliella has species with wide salinity tolerance, but the mechanism of excess Na+ removal in cells is still debated. Through studying the expression of DmHA1 and DmHA2 under hyperosmotic salt shock, it was found that DmHA2 plays a crucial role in overcoming salt stress.
Article
Biochemistry & Molecular Biology
Ryotaro Notomi, Shigeki Sasaki, Yosuke Taniguchi
Summary: Triplex DNA formation is a site-specific recognition method for duplex DNA, but it lacks natural nucleotides for recognizing certain base pairs. Therefore, the development of triplex-forming oligonucleotides (TFOs) with artificial nucleosides is important. In this study, researchers designed and synthesized new nucleoside derivatives based on the 2'-deoxy-nebularine (dN) skeleton. They successfully demonstrated that TFOs with 2-guanidinoethyl-2'-deoxynebularine (guanidino-dN) recognized (5m)CG and CG base pairs with high affinity in different DNA sequences. This finding has biological importance for gene regulation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
James R. Sayer, Karin Wallden, Thomas Pesnot, Frederick Campbell, Paul J. Gane, Michela Simone, Hans Koss, Floris Buelens, Timothy P. Boyle, David L. Selwood, Gabriel Waksman, Alethea B. Tabor
BIOORGANIC & MEDICINAL CHEMISTRY
(2014)
Review
Cell Biology
Karin Wallden, Angel Rivera-Calzada, Gabriel Waksman
CELLULAR MICROBIOLOGY
(2010)
Article
Multidisciplinary Sciences
Karin Wallden, Robert Williams, Jun Yan, Pei W. Lian, Luchun Wang, Konstantinos Thalassinos, Elena V. Orlova, Gabriel Waksman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
Karin Wallden, Tomas Nyman, B. Martin Hallberg
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
James R. Sayer, Karin Wallden, Hans Koss, Helen Allan, Tina Daviter, Paul J. Gane, Gabriel Waksman, Alethea B. Tabor
Summary: H. pylori infections are linked to gastric ulcers and cancers, with challenges in current therapies due to antibiotic resistance. Studies focus on inhibiting HP0525 and exploring potential bivalent inhibitors. Through various methods and structural details of subunit interactions, the aim is to improve selectivity and develop these compounds as tools for probing the assembly of the HP0525 hexamer.
JOURNAL OF PEPTIDE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Karin Wallden, Agnes Rinaldo-Matthis, Benedetta Ruzzenente, Chiara Rampazzo, Vera Bianchi, Par Nordlund
Article
Biochemistry & Molecular Biology
Karin Wallden, Pal Stenmark, Tomas Nyman, Susanne Flodin, Susanne Graslund, Peter Loppnau, Vera Bianchi, P. Nordlund
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Biochemistry & Molecular Biology
K Walldén, B Ruzzenente, A Rinaldo-Matthis, V Bianchi, P Nordlund
Article
Plant Sciences
Annemarie Perez Boerema, Shintaro Aibara, Bijoya Paul, Victor Tobiasson, Dari Kimanius, Bjorn O. Forsberg, Karin Wallden, Erik Lindahl, A. Amunts
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)