Article
Pharmacology & Pharmacy
Baolong Pan, Fanpeng Kong, Xiaofen Ju, Jing Song, Linping Wang, Qiao Niu, Xiaoting Lu
Summary: Aluminum is recognized as a neurotoxin that induces tau hyperphosphorylation. The carboxyl terminus of Hsc70-interacting protein (CHIP) plays an important role in regulating tau hyperphosphorylation induced by aluminum chloride (AlCl3) in N2a cells. Both the U-box and TPR domains of CHIP are indispensable and play an important role in this regulation.
Article
Biochemistry & Molecular Biology
Hana Popelka, Vikramjit Lahiri, Wayne D. Hawkins, Felipe da Veiga Leprevost, Alexey I. Nesvizhskii, Daniel J. Klionsky
Summary: The intrinsically disordered protein region (IDPR) of Atg12 protein plays an important role in its functional structure, as it is positioned in proximity to the ubiquitin-like (UBL) domain. Deletion in IDPR disrupts the integrity of the UBL domain, while a mutation in the predicted α0 helix in IDPR prevents Atg12 from binding to Atg7 and Atg10, impacting its function in the ubiquitin-like conjugation cascade.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Morihiko Nakamura, Yuki Fukuma, Kaori Notsu, Megumi Kono
Summary: Quercetin suppresses nitric oxide production in macrophages and regulates cellular signaling by influencing the action of MNSF beta and HSC70. MNSF beta is involved in TLR4/MyD88 signaling, which is regulated by HSC70 and negatively controlled by quercetin.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Nikola Winter, Maria Novatchkova, Andreas Bachmair
Summary: The first amino acid of a protein plays a significant role in its metabolic stability, with N-degrons mediating turnover. Despite only a few proteases known to contribute to the generation of N-degrons, there is potential for further discovery in many processing paths.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Adam J. Kanack, Michael D. Olp, Oliver J. Newsom, Jamie B. Scaglione, David M. Gooden, Kevin McMahon, Brian C. Smith, K. Matthew Scaglione
Summary: The ubiquitin ligase CHIP plays an important role in proteostasis, but few small molecules that regulate its activity have been developed. In this study, we identified a compound called MS.001 that inhibits the chaperone binding and ubiquitin ligase activity of CHIP. Our data also reveal a potential binding site for MS.001 on the linker domain of CHIP.
Article
Biochemistry & Molecular Biology
Yovana Cabrera, Ganeko Bernardo-Seisdedos, Leire Dublang, David Albesa-Jove, Natalia Orozco, Ana Rosa Viguera, Oscar Millet, Arturo Muga, Fernando Moro
Summary: This study reveals the role of Apg2 in regulating the activity of human Hsc70, and its C-terminal extension acts as a fine-tuner of substrate remodeling ability in the chaperone system.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemical Research Methods
Sunkyu Choi, Atilio Reyes Romero, Fathima Mashood, Neha Goswami, Lotfi Chouchane, Frank Schmidt
Summary: Protein turnover, the balance between protein synthesis and degradation, is essential for cellular protein homeostasis. This study aimed to analyze protein turnover in adipocytes using time-dependent SILAC labeling. The results revealed associations between protein half-lives and various cellular processes, including energy metabolism, RNA splicing, and Golgi complex. These findings contribute to a better understanding of adipocyte proteome dynamics and protein homeostasis maintenance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Yoonsung Joh, Kangbae Lee, Hyunwoo Kim, Heejin Park
Summary: A novel approach, called progressive search, efficiently obtains analysis results for updated databases in tandem mass spectrometry. Compared to performing a normal search from scratch, progressive search achieves the same results much faster.
BMC BIOINFORMATICS
(2023)
Article
Biochemical Research Methods
Jorvani Cruz Villarreal, Rory Kruithoff, Ana Egatz-Gomez, Paul D. Coleman, Robert Ros, Todd R. Sandrin, Alexandra Ros
Summary: Understanding cell-to-cell variation at the molecular level is critical for research and this study presents a microfluidic platform combined with MALDI-TOF-MS for the detection and quantification of targeted proteins from small cell ensembles. The integrated on-chip approach allows for protein quantification from cell lysate and demonstrates promising potential for single-cell analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fengxia Li, Eugenio F. Fornasiero, Tal M. Dankovich, Verena Kluever, Silvio O. Rizzoli
Summary: By systematically studying probes initially developed for electron microscopy, researchers found that antibodies coupled to 6 nm gold particles exhibit excellent labeling specificity and reliable detection threshold in nanoSIMS imaging. These tools enable accurate visualization and analysis of samples and can be easily combined with other imaging approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Fernanda Rodrigues Spinelli, Ana Paula Drehmer, Leonardo Valentin, Simone Nascimento, Mariana Baptistao, Regina Vanderlinde
Summary: A sample preparation approach for analyzing alpha-casein, beta-casein, albumin, and lysozyme was developed and validated in this study. The method showed high recovery values and could quantify proteins even at lower levels than the current legal limits.
Article
Neurosciences
Yong Liu, You Wang, Juan Yang, Tao Xu, Changhong Tan, Peng Zhang, Qiankun Liu, Yangmei Chen
Summary: The study identified that GIPC1 is downregulated in patients with epilepsy and mice with induced epilepsy, and it interacts with mGluR7 to regulate epileptogenesis by increasing mGluR7 expression.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Chemistry, Medicinal
Hao Shao, Xiaokai Li, Shigenari Hayashi, Jeanette L. Bertron, Daniel M. C. Schwarz, Benjamin C. Tang, Jason E. Gestwicki
Summary: The research team synthesized 17 derivatives of YM-08, among which compound JG-23 showed 12-fold better metabolic stability and retained the ability to reduce tau levels in cell models. These chemical probes are expected to be useful tools for studying tau homeostasis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Review
Biochemical Research Methods
Sven Heiles
Summary: Metabolomics and lipidomics are driving forces in the omics era, with tandem mass spectrometry methods playing a crucial role in characterizing the structural details of metabolites and lipids. Recent advancements in alternative tandem MS methodologies aim to provide full structural characterization of these compounds. The review critically examines the principles, capabilities, drawbacks, and initial applications of these advanced tandem mass spectrometry strategies in the fields of metabolomics and lipidomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Gregoire Quinet, Pierre Genin, Oznur Ozturk, Naima Belgareh-Touze, Lilas Courtot, Renaud Legouis, Robert Weil, Mickael M. Cohen, Manuel S. Rodriguez
Summary: The regulation of autophagy pathway and the development of LC3-traps, which can capture target proteins from various sources, have been studied. The LC3-traps have potential applications in monitoring selective autophagy events.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)