Article
Biochemistry & Molecular Biology
Alexa Harnagel, Landys Lopez Quezada, Sae Woong Park, Catherine Baranowski, Karen Kieser, Xiuju Jiang, Julia Roberts, Julien Vaubourgeix, Amy Yang, Brock Nelson, Allison Fay, Eric Rubin, Sabine Ehrt, Carl Nathan, Tania J. Lupoli
Summary: Research has shown that in Mycobacterium tuberculosis (Mtb), bacterial chaperones ClpB and DnaK play important roles in responding to host stress conditions, interacting with other heat shock proteins and stress response genes. Additionally, the loss of both chaperones leads to impaired recovery after exposure to stressors such as oxidants, especially reactive nitrogen species, indicating that inhibition of multiple chaperones could effectively disable Mtb in concert with host immunity.
MOLECULAR MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Athar Alam, Jeanette E. Broms, Rajender Kumar, Anders Sjostedt
Summary: Bacterial survival within a mammalian host relies on sensing environmental perturbations and initiating appropriate counter-responses, where bacterial chaperone systems, particularly ClpB, play indispensable roles. ClpB is crucial for bacterial survival under various stress conditions, especially heat shock, and also regulates secretion of bacterial effector molecules related to type VI secretion systems.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Kevin Reinle, Axel Mogk, Bernd Bukau
Summary: The protein quality control system maintains protein homeostasis through substrate degradation, refolding, and sequestration activities, in which small heat shock proteins play a crucial role and adapt to different tasks and stress conditions.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Shannon McMahon, Steven Bergink, Harm H. Kampinga, Heath Ecroyd
Summary: The study shows that DNAJB proteins can inhibit protein aggregation by supporting the Hsp70-dependent degradation pathway, different from the mechanism of preventing fibrillar aggregation. The research also found that DNAJB6 and DNAJB8 have two distinct regions for binding substrates.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Arjun Saha, Arieh Warshel
Summary: This study explored the molecular basis of substrate translocation by the AAA+ motor of the 26S proteasome using simulation approaches, revealing the role of electrostatic interactions and validating the involvement of bulkier residues in pore loop 1 in substrate translocation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Huanhuan Xiao, Hongying Wang, Qiuxia He, Jianfeng Zhou, Shaojun Du
Summary: The expression and function of Aha1 in zebrafish were characterized in this study. It was found that Aha1 showed distinct expression patterns during zebrafish embryonic development and was significantly upregulated in response to heat shock. Furthermore, Aha1 was found to be dispensable for zebrafish embryonic development, growth, and survival.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Prapansak Srisapoome, Kubpaphas Thummabancha, Ratree Wongpanya
Summary: This study successfully cloned and characterized two cDNAs encoding Nile tilapia DnaJ proteins, revealing their gene structures and tissue expression patterns under normal and pathogenic infection conditions. Silencing of these genes confirmed their crucial roles in response to heat and bacterial stress, suggesting their involvement in regulating intracellular proteins during infection.
Article
Medicine, Research & Experimental
Yanxin Fan, Marta Murgia, Monika Linder, Yoko Mizoguchi, Cong Wang, Marcin Lyszkiewicz, Natalia Zietara, Yanshan Liu, Stephanie Frenz, Gabriela Sciuccati, Armando Partida-Gaytan, Zahra Alizadeh, Nima Rezaei, Peter Rehling, Sven Dennerlein, Matthias Mann, Christoph Klein
Summary: The relevance of molecular mechanisms governing mitochondrial proteostasis to the differentiation and function of hematopoietic and immune cells is largely elusive. Through the study of proteins related to HCLS1-associated protein X-1, a potentially novel CLPB/HAX1/(PRKD2)/HSP27 axis was identified to play an important role in the differentiation of neutrophil granulocytes. This study provides insights into the molecular and metabolic mechanisms underlying congenital neutropenia and sheds light on the critical role of mitochondrial proteostasis in the differentiation and function of neutrophil granulocytes.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Materials Science, Multidisciplinary
Ana Rodriguez-Ramos, Miguel A. Ramos-Docampo, Veronica Salgueirino, Monica L. Fanarraga
Summary: One of the futuristic challenges in nanomedicine is to create self-propelled nanorobots to scan and repair living tissues. Researchers have combined nanotechnology and biotechnology to design a biocompatible propulsion system based on the molecular chaperone Hsp90. This propulsion mechanism could be used to design nanodevices capable of performing sophisticated tasks in live biological contexts.
MATERIALS TODAY ADVANCES
(2023)
Review
Biochemistry & Molecular Biology
Grace Lee, Rebecca S. Kim, Sang Bum Lee, Sukyeong Lee, Francis T. F. Tsai
Summary: Hsp100 chaperones, also known as Clp proteins, are a family of ATPases that play important roles in cells and organelles by using metabolic energy to unfold proteins and break down protein aggregates. Recent research has provided new mechanistic insights into the structure and function of this group of chaperones.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Microbiology
Paolo Costa, Giulia Usai, Angela Re, Marcello Manfredi, Giuseppe Mannino, Cinzia Margherita Bertea, Enrica Pessione, Roberto Mazzoli
Summary: Comparative gel-free proteomics analysis of Clostridium cellulovorans exposed to butanol revealed modulation of protein biosynthesis, up-regulation of ATPases, and rearrangement of cell envelope, suggesting potential gene targets for metabolic engineering strategies aimed at improving butanol tolerance in this microbial strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Michal Zolkiewski
Summary: ClpB, a molecular chaperone in pathogenic bacteria, plays a crucial role in their survival and infection under host-induced stress, making it a promising target for novel antimicrobial therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Panagiotis Katikaridis, Valentin Bohl, Axel Mogk
Summary: Bacteria, as unicellular organisms, face environmental changes directly and use disaggregases to solubilize and reactivate aggregated proteins for survival and growth. ClpB and ClpG are two well-characterized bacterial disaggregases with different mechanisms and regulatory modes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Hye-Youn Kim, Suntaek Hong
Summary: Heat shock proteins (HSPs), particularly the DNAJB proteins within the HSP40 family, play crucial roles in cancer development and metastasis by regulating and dysregulating tumor progression at various levels. Understanding the differences in function and regulatory mechanisms of DNAJB proteins offers new insights into tumorigenesis and metastasis, thereby improving therapeutic opportunities for malignant diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Mangesh Damre, Ashan Dayananda, Rohith Anand Varikoti, George Stan, Ruxandra Dima
Summary: The study investigates the dynamic stability and interactions of disaggregation and microtubule-severing nanomachines using extensive molecular dynamics simulations, revealing distinct substrate processing mechanisms of the two severing proteins ClpB and spastin compared to katanin. The simulations also highlight the importance of salt bridges in maintaining the stability of hexameric pores and intraring interactions, and suggest observation-time-dependent relaxation dynamics in the distance between loops and protomers.
BIOPHYSICAL JOURNAL
(2021)
Letter
Microbiology
Sabina Kedzierska-Mieszkowska
VETERINARY MICROBIOLOGY
(2015)
Article
Microbiology
Joanna Krajewska, Zbigniew Arent, Daniel Wieckowski, Michal Zolkiewski, Sabina Kedzierska-Mieszkowska
Article
Biochemistry & Molecular Biology
Joanna Krajewska, Zbigniew Arent, Michal Zolkiewski, Sabina Kedzierska-Mieszkowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Letter
Endocrinology & Metabolism
Sabina Kedzierska-Mieszkowska, Joanna Renke
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2018)
Article
Biochemistry & Molecular Biology
Joanna Renke, Sabina Kedzierska-Mieszkowska, Magdalena Lange, Boguslaw Nedoszytko, Anna Liberek, Katarzyna Plata-Nazar, Marcin Renke, Tomasz Wenta, Dorota Zurawa-Janicka, Joanna Skorko-Glonek, Barbara Lipinska
ACTA BIOCHIMICA POLONICA
(2018)
Article
Biochemistry & Molecular Biology
Kamila Zblewska, Joanna Krajewska, Michal Zolkiewski, Sabina Kedzierska-Mieszkowska
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2014)
Article
Multidisciplinary Sciences
Ting Zhang, Sabina Kedzierska-Mieszkowska, Huitao Liu, Chuanmin Cheng, Roman R. Ganta, Michal Zolkiewski
Article
Multidisciplinary Sciences
Joanna Krajewska, Anna Modrak-Wojcik, Zbigniew J. Arent, Daniel Wieckowski, Michal Zolkiewski, Agnieszka Bzowska, Sabina Kedzierska-Mieszkowska
Review
Medicine, Research & Experimental
Joanna Renke, Sabina Kedzierska-Mieszkowska, Magdalena Lange, Boguslaw Nedoszytko, Eliza Wasilewska, Anna Liberek, Marcin Renke, Marek Niedoszytko, Jacek Witkowski, Joanna Skorko-Glonek, Barbara Lipinska
ADVANCES IN MEDICAL SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Katarzyna Potrykus, Zbigniew Arent, Joanna Krajewska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Medicine, General & Internal
Joanna Renke, Eliza Wasilewska, Sabina Kedzierska-Mieszkowska, Katarzyna Zorena, Sylwia Baranska, Tomasz Wenta, Anna Liberek, Danuta Siluk, Dorota Zurawa-Janicka, Aleksandra Szczepankiewicz, Marcin Renke, Barbara Lipinska
MEDICINA-LITHUANIA
(2020)
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Zbigniew Arent
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska, Michal Zolkiewski
Summary: ClpB, a molecular chaperone in pathogenic bacteria, plays a crucial role in their survival and infection under host-induced stress, making it a promising target for novel antimicrobial therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Sabina Kedzierska-Mieszkowska, Zbigniew Arent
Summary: L. interrogans is a highly invasive spirochaete causing leptospirosis in mammals. During infection, it reprograms gene expression to survive and establish infection. LIC_10559, a putative ECF sigma factor, is likely to be active during infection. In this study, it was found that LIC_10559 can induce the host's immune response, suggesting its involvement in the pathogenesis of leptospirosis.
Review
Biochemistry & Molecular Biology
Sabina Kedzierska-Mieszkowska
Summary: This review aims to summarize the current understanding of the role of s factors in Leptospira interrogans, a highly invasive spirochaete responsible for leptospirosis. The disease has a significant impact on public health and the global economy. S factors are key regulators of gene expression in bacteria and play a crucial role in bacterial adaptative response to different environmental stimuli. The L. interrogans genome encodes a total of 14 s factors, including one housekeeping factor and three types of alternative factors. The characteristics and possible role of these factors in gene regulation and pathogen adaptability are discussed.
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)