期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 403, 期 5, 页码 763-776出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2010.09.025
关键词
3D reconstruction; fish skeletal muscle; bare zone region; M-region; Myosin filament
资金
- British Heart Foundation [FS/07/017/22951, PG/07/076/23480]
- Wellcome Trust University [066418]
- National Institutes of Health [S06 GM08224]
- National Centre for Research Resources, National Institutes of Health [G12RR-03051]
- European MYORES Network
- British Heart Foundation [PG/07/076/23480, FS/07/017/22951] Funding Source: researchfish
The rods of anti-parallel myosin molecules overlap at the centre of bipolar myosin filaments to produce an M-region (bare zone) that is free of myosin heads Beyond the M-region edges, myosin molecules aggregate in a parallel fashion to yield the bridge regions of the myosin filaments Adjacent myosin filaments in striated muscle A-bands are cross-linked by the M-band Vertebrate striated muscle myosin filaments have a 3-fold rotational symmetry around their long axes In addition, at the centre of the M-region, there are three 2-fold axes perpendicular to the filament long axis, giving the whole filament dihedral 32-point group symmetry Here we describe the three-dimensional structure obtained by a single-particle analysis of the M-region of myosin filaments from goldfish skeletal muscle under relaxing conditions and as viewed in negative stain This is the first single-particle reconstruction of isolated M-regions The resulting three-dimensional reconstruction reveals details to about 55 angstrom resolution of the density distribution in the five main nonmyosin densities in the M-band (M6', M4', M1, M4 and M6) and in the myosin head crowns (P1, P2 and P3) at the M-region edges The outermost crowns in the reconstruction were identified specifically by their close similarity to the corresponding crown levels in our previously published bridge region reconstructions The packing of myosin molecules into the M-region structure is discussed, and some unidentified densities are highlighted (C) 2010 Elsevier Ltd All rights reserved
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