期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 385, 期 3, 页码 788-799出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2008.11.008
关键词
end joining; DSB repair; PC4; atomic force microscopy; NHEJ
资金
- Department of Science and Technology
- Department of Biotechnology, Government of India, India-Japan Cooperative Science Programme
- Jawaharlal Nehru Center for Advanced Scientific Research
- Council of Scientific and Industrial Research Senior Research Fellow
Human transcriptional coactivator PC4 is a highly abundant nuclear protein that is involved in diverse cellular processes ranging from transcription to chromatin organization. Earlier, we have shown that PC4, a positive activator of p53, overexpresses upon genotoxic insult in a p53-dependent manner. In the present study, we show that PC4 stimulates ligase-mediated DNA end joining irrespective of the source of DNA ligase. Pull-down assays reveal that PC4 helps in the association of DNA ends through its C-terminal domain. In vitro nonhomologous end-joining assays with cell-free extracts show that PC4 enhances the joining of noncomplementary DNA ends. Interestingly, we found that PC4 activates double-strand break (DSB) repair activity through stimulation of DSB rejoining in vivo. Together, these findings demonstrate PC4 as an activator of nonhomologous end joining and DSB repair activity. (C) 2008 Elsevier Ltd. All rights reserved.
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