Article
Chemistry, Analytical
Ui Jin Lee, Yunkwang Oh, Oh Seok Kwon, Yong-Beom Shin, Moonil Kim
Summary: We developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system. The system utilizes a galactose/glucose-binding protein (GGBP) with a N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The system demonstrated ultra-high sensitivity and selective responses for IAV detection, making it a simple and effective platform for IAV detection without the need for virus culture or RNA extraction processes.
Review
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Guoyun Sun, Rodrigo Ledesma-Amaro, Long Liu
Summary: This review presents the pivotal role of microscopy imaging of living cells in metabolic engineering and its applications in industrial microorganisms. It analyzes the advantages and disadvantages of different microscopy technologies and their roles in synthetic biology. Lastly, it discusses the future perspectives of live-cell imaging and its potential in transforming microbial systems.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Yuao Sun, Yao Wang, Keyang Chen, Yujie Sun, Sheng Wang
Summary: In this study, we developed a bright stable green fluorescent protein, Springgreen-M, and established a novel BiFC assay based on Springgreen-M for imaging protein-protein interactions (PPIs) in live cells with high specificity. By combining the same lineage, multiplexing imaging of PPIs could also be achieved in the same live cell. The newly developed Springgreen-M and Springgreen-M-based BiFC probes meet the urgent need for high-specificity BiFC detection, flexible visualization, and screening of PPIs in live cells.
Article
Plant Sciences
Yuanyuan Jiang, Jiangrong Peng, Yunpeng Cao, Zhiqiang Han, Ling Zhang, Wenbing Su, Shunquan Lin, Yuan Yuan, Bin Wang, Xianghui Yang, Zhike Zhang
Summary: The study introduced a new method for observing the cell structure of Nicotiana benthamiana, significantly improving imaging by fluorescence microscopy and allowing clear visualization of cellular structures and protein interactions under high-power lens. This method provides insights and references for the study of cell structures and functions in other plants, demonstrating its applicability in live cell visualization through confocal scanning laser microscopy.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Biochemical Research Methods
Shintaro Ichikawa, Shota Kato, Yuta Fujii, Kazuya Ishikawa, Keiji Numata, Yutaka Kodama
Summary: In this study, we artificially controlled chloroplast-chloroplast interactions in living plant cells using the organelle glue (ORGL) technique. By targeting different fragments of a fluorescent protein to specific locations, we modulated the frequency of chloroplast-chloroplast interactions.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemical Research Methods
Florent Velay, Melanie Soula, Marwa Mehrez, Clement Belbachir, Stefano D'Alessandro, Christophe Laloi, Patrice Crete, Ben Field
Summary: This study describes the development of a flexible modular cloning-based toolkit for bimolecular fluorescence complementation (BiFC) and proximity labelling in the chloroplast and other cellular compartments. The toolkit simplifies the construction of chloroplast fusion proteins, enables robust ratiometric quantification, and provides model positive and negative controls. The study highlights potential pitfalls in designing BiFC experiments, such as the choice of FP split, negative controls, cell type, and reference FP.
Article
Chemistry, Multidisciplinary
Feng Liu, Huimin Hu, Mengying Deng, Zongqin Xiang, Yuting Guo, Xinmeng Guan, Dong Li, Qinxue Hu, Wenliang Lei, Hongjuan Peng, Jun Chu
Summary: This study reports a bright monomeric near-infrared fluorescent protein, mIFP663, with maximum excitation at 633 nm. It has high cellular brightness and can label critical cellular and viral proteins without affecting subcellular localization and virus replication. Additionally, mIFP663 can be used to improve bimolecular fluorescence complementation and bioluminescent resonance energy transfer systems for detecting protein-protein interactions in living cells.
Article
Genetics & Heredity
Ashley S. Denney, Andrew D. Weems, Michael A. McMurray
Summary: Life necessitates the oligomerization of individual proteins into higher-order assemblies with the help of molecular chaperones. Missense mutations can disrupt protein folding and lead to disease, but some mutations are kinetically delayed to prevent interference with wild-type function. The involvement of chaperones, kinetic folding delays, and oligomerization dynamics were further explored in tumor-derived mutants of the tumor suppressor p53, with findings suggesting temperature sensitivity and Hsp90 regulation.
G3-GENES GENOMES GENETICS
(2021)
Article
Plant Sciences
Li Guo, Alina Klaus, Marcel Baer, Gwendolyn K. Kirschner, Silvio Salvi, Frank Hochholdinger
Summary: This study demonstrates the central role of the EGT2 gene and its interaction partners in controlling the root zone-specific transcriptomic reprogramming of barley roots upon gravistimulation. The genes directly interacting with EGT2 are involved in cell wall and reactive oxygen species-related processes. These findings are important for the development of improved crop performance through manipulation of root traits.
Article
Cell Biology
Sarai Castro-Bustos, Israel Maruri-Lopez, Maria Azucena Ortega-Amaro, Mario Serrano, Cesare Ovando-Vazquez, Juan Francisco Jimenez-Bremont
Summary: This study analyzed the subcellular localization and potential interactors of the Arabidopsis thaliana glycine-rich domain protein 2 (AtGRDP2). It was found that the AtGRDP2 protein is localized in the cytosol and nucleus, and the DUF1399 or RRM domains are sufficient for nuclear localization. Furthermore, heterodimeric interactions between AtGRDP2 and specific proteins were observed in different compartments, suggesting the involvement of AtGRDP2 in post-transcriptional processes.
CELL STRESS & CHAPERONES
(2022)
Article
Biochemistry & Molecular Biology
Zhonggang Shi, Xing Gao, Wenrui Zhang, Binghong Chen, Mengying Wang, Keman Liao, Zhihan Wang, Li Ren, Yujia Zhai, Yongming Qiu, Xuhui Wang, Yingying Lin
Summary: Investigations of protein-protein interactions are crucial for understanding cellular processes. This study introduces TagBFP2 into the BiFC assay, which performs better than EYFP in terms of signal-to-noise ratio. This method is straightforward, reliable, and time-efficient.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ozge Ugurlu, Serap Evran
Summary: YopM, an essential effector protein of Yersinia, shows immunosuppressive effect through protein-protein interactions in host cells. This study investigated the protein-protein interactions of YopM with T3SS components LcrV and LcrG, and found that YopM interacts with LcrG. BiFC assay is proposed as a simple method to screen novel interaction partners of YopM.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Neelima Boora, Vibha Verma, Ridhi Khurana, Gautam Gawande, Sanchi Bhimrajka, Komal Chaprana, Meenu Kapoor, Sanjay Kapoor
Summary: The importance of protein-protein interactions in cellular processes is highlighted, along with a demonstration of a method for visualizing tripartite interactions using Fluorescence Lifetime Imaging. This methodology allows dynamic observation of protein interactions and their impact on cellular functions in vivo.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Biotechnology & Applied Microbiology
Chih Hung Lo
Summary: Tau protein plays a crucial role in physiological functions but is also implicated in Alzheimer's disease and tauopathies. Recent studies have highlighted soluble tau oligomers as the primary toxic species. The use of cellular biosensor technology has advanced our understanding of tau oligomerization and aggregation, providing insights into tau conformational ensembles and propagation of pathology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Plant Sciences
Timothee Laloux, Irwin Matyjaszczyk, Simon Beaudelot, Charles Hachez, Francois Chaumont
Summary: It was found that the interaction between SYP121 and PIP2;7 depends on specific structural regions, rather than individual Habc or Qa-SNARE domains. Deletion of the N- and/or C-terminus of the PIP2;7 protein leads to a decrease in interaction with SYP121. This suggests that the interaction motifs in both SYP121 and PIP2;7 depend on the protein conformation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Yunhui Cheng, Raili Emilia Kerppola, Tom Klaus Kerppola
ENDOCRINE-RELATED CANCER
(2016)
Article
Cell Biology
Ho-Pi Lin, Claudius Vincenz, Kevin W. Eliceiri, Tom K. Kerppola, Brenda M. Ogle
BIOLOGY OF THE CELL
(2010)
Article
Developmental Biology
Huai Deng, Tom K. Kerppola
Article
Oncology
Sebastian Susperreguy, Luciana P. Prendes, Maria A. Desbats, Nancy L. Charo, Karen Brown, Ormond A. MacDougald, Tom Kerppola, Jessica Schwartz, Graciela Piwien-Pilipuk
EXPERIMENTAL CELL RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
Veronica Burns, Tom Klaus Kerppola
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Neurosciences
Paul M. Jenkins, Jeremy C. McIntyre, Lian Zhang, Arun Anantharam, Eileen D. Vesely, Kristin L. Arendt, Cynthia J. L. Carruthers, Tom K. Kerppola, Jorge A. Iniguez-LluhI, Ronald W. Holz, Michael A. Sutton, Jeffrey R. Martens
JOURNAL OF NEUROSCIENCE
(2011)
Article
Biochemistry & Molecular Biology
Xiaojun Ren, Tom K. Kerppola
MOLECULAR AND CELLULAR BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Shoumei Bai, Tom K. Kerppola
MOLECULAR AND CELLULAR BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Bo Cheng, Xiaojun Ren, Tom K. Kerppola
MOLECULAR AND CELLULAR BIOLOGY
(2014)
Article
Genetics & Heredity
Huai Deng, Tom K. Kerppola
Retraction
Biochemistry & Molecular Biology
Shoumei Bai, Tom K. Kerppola
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Immunology
Veronica Elizabeth Burns, Tom Klaus Kerppola
Summary: Keap1 binds to cytokine genes upon virus infection and moderates their induction by recruiting NF-kappa B p50 and G9a-GLP. This suggests a feedback circuit between Keap1, NF-kappa B, and G9a-GLP that helps moderate viral induction of cytokine transcription.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Veronica Elizabeth Burns, Tom Klaus Kerppola
BRITISH JOURNAL OF PHARMACOLOGY
(2017)
Meeting Abstract
Biochemistry & Molecular Biology
Veronica E. Burns, Tom K. Kerppola
Meeting Abstract
Clinical Neurology
Masayoshi Tada, Tom K. Kerppola, Stuart J. Decker, Sokol V. Todi, Matthew K. Scaglione, Maria do Carmo P. Costa, Henry Paulson
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)