Article
Multidisciplinary Sciences
Emily Z. Guo, Jorge E. Galan
Summary: Type III secretion systems are multiprotein molecular machines essential for the virulence of bacterial pathogens. The injectisome mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The structure of the needle tip complex and its interaction with the needle filament are crucial for the translocation of effector proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Zhi Xiong Zeng, Lu Yi Liu, Shui Bing Xiao, Jin Fang Lu, Ying Li Liu, Jing Li, Yuan Ze Zhou, Li Jing Liao, Duan You Li, Ying Zhou, Pin Nie, Hai Xia Xie
Summary: This study reveals the interaction between EscE and EsaH in Edwardsiella piscicida, and their role in stabilizing the T3SS needle protein EsaG. The EscE-EsaH complex acts as cochaperones and controls the secretion of T3SS needle protein EsaG, which in turn leads to the secretion of middle and late T3SS substrates. This research provides new insights into the regulation of protein secretion in E. piscicida.
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Hanh N. Lam, Tannia Lau, Adam Lentz, Jessica Sherry, Alejandro Cabrera-Cortez, Karen Hug, Annalyse Lalljie, Joanne Engel, R. Scott Lokey, Victoria Auerbuch
Summary: The development of new antibiotics is urgently needed due to the emerging threat of antibiotic-resistant bacteria. A newly discovered isomer, 4EpDN, shows potent inhibitory effects on multiple Gram-negative bacteria's injectisome type III secretion system (T3SS), possibly by preventing complete T3SS assembly.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
Guillain Mikaty, Christian E. Demeure, Sofia Filali, Javier Pizarro-Cerda, Pierre Goossens, Elisabeth Carniel
Summary: This study aimed to determine whether DIC occurs at the late stages of plague and anthrax. The results showed that animals infected with anthrax exhibited typical DIC characteristics, while those infected with plague did not. Therefore, understanding the mechanisms behind these hemorrhages is crucial.
MICROBES AND INFECTION
(2023)
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Review
Biochemistry & Molecular Biology
Raysa Rosario-Acevedo, Sergei S. Biryukov, Joel A. Bozue, Christopher K. Cote
Summary: Plague, caused by the bacterial pathogen Yersinia pestis, is a vector-borne disease that continues to infect humans worldwide. Research on vaccines and therapeutics for plague is important for infection mitigation and disease treatment, as plague remains a public health threat and biodefense concern.
Article
Multidisciplinary Sciences
Hailian Wu, Haisheng Wu, Yongshun Wang, Hongying Li, Fuzhang Tian, Kuizhang Zhou, Zhizhen Qi, Yiquan Zhang, Qingwen Zhang, Xuefei Zhang
Summary: This study developed a Himalayan marmot information collection system based on 3S technology and V3.0, and drew a spatial distribution map of Himalayan marmots in Qinghai Province. The accuracy of the map was validated through field data collection, leading to improved efficiency in plague surveillance and reduced workload for researchers.
SCIENTIFIC REPORTS
(2023)
Review
Microbiology
R. Barbieri, M. Signoli, D. Cheve, C. Costedoat, S. Tzortzis, G. Aboudharam, D. Raoult, M. Drancourt
Summary: Plague can be transmitted through different routes, including fecal-oral, respiratory, and ingestion routes. Control of plague infection relies on early diagnosis, antibiotic treatment, and isolation measures. The disease has existed for at least 5,000 years, with well-established monitoring and prevention measures in Eurasia.
CLINICAL MICROBIOLOGY REVIEWS
(2021)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Julia E. Page, Meredith A. Skiba, Truc Do, Andrew C. Kruse, Suzanne Walker
Summary: Construction and remodeling of the bacterial peptidoglycan cell wall is essential for cell growth and division. Hydrolases, such as LytH in Staphylococcus aureus, play a crucial role in this process and can be potential targets for new antibiotics. The protein partner, ActH, is required for LytH activity and its amidase-activating function is found in its extracellular tetratricopeptide repeats (TPRs). Our findings suggest that metal cofactor stabilization is a general strategy used by amidase activators and ActH functions as a multi-functional protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)