Article
Cell Biology
Ignacio Garcia, Sara Orellana-Munoz, Lucia Ramos-Alonso, Aram N. Andersen, Christine Zimmermann, Jens Eriksson, Stig Ove Boe, Petra Kaferle, Manolis Papamichos-Chronakis, Pierre Chymkowitch, Jorrit M. Enserink
Summary: Cells use mechanisms to detect signals and respond, with Kel1 serving as a noise suppressor in the yeast pheromone pathway, preventing cell death by inhibiting membrane protein recruitment to prevent spontaneous activation of the pathway.
Correction
Biochemistry & Molecular Biology
R. J. Sullivan, K. Flaherty
Summary: A correction to the paper has been published.
Article
Immunology
Lauren C. Kinkead, Samantha J. Krysa, Lee-Ann H. Allen
Summary: This study investigated the contributions of ERK and p38 MAP kinase, PI3K, AKT, and NF-kappa B to neutrophil survival. The results showed that p38 alpha, PI3K alpha, and NF-kappa B play important roles in the delayed apoptosis of infected cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Genetics & Heredity
Marisa Madrid, Elisa Gomez-Gil, Jose Cansado
Summary: Research has shown that the core component of the stress-activated pathway, p38 MAPK ortholog Sty1, negatively modulates CAR assembly and integrity in S. pombe under environmental stress and actin cytoskeletal damage, while positively reinforcing CAR assembly in S. japonicus. This opposite effect of SAPK signaling on CAR integrity may represent an evolutionary refined adaptation to cope with differences in cytokinesis onset in both fission yeast species.
Article
Multidisciplinary Sciences
Deepak Mishra, Tristan Bepler, Brian Teague, Bonnie Berger, Jim Broach, Ron Weiss
Summary: The research team created a bistable toggle switch in Saccharomyces cerevisiae using a cross-repression topology, and developed a computational framework to search for and validate other large and similar bistable networks. This highlights the potential of building synthetic protein-protein networks.
Article
Multidisciplinary Sciences
Lanlan Wang, Guojuan Xu, Lihua Li, Meiying Ruan, Anne Bennion, Guo-Liang Wang, Ran Li, Shaohong Qu
Summary: Receptor-like kinases (RLKs) in plants play diverse roles in plant development and stress responses by perceiving and transmitting environmental signals. In this study, a negative regulator named BDR1 was identified as a key player in rice resistance to the blast fungus Magnaporthe oryzae. BDR1 interacts with and phosphorylates MPK3, negatively regulating several defense-related pathways including jasmonate (JA) signaling and terpenoid biosynthesis. These findings contribute to our understanding of the molecular mechanisms underlying rice blast resistance.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Jason F. Cooper, Ryan J. Guasp, Meghan Lee Arnold, Barth D. Grant, Monica Driscoll
Summary: The study found that in Caenorhabditis elegans model, neurons under oxidative and osmotic stress can expel toxic protein aggregates through large vesicles called exophers. Additionally, fasting can significantly increase this clearance process. Furthermore, a nonautonomous network was identified to connect changes in food availability with remote neuronal responses, revealing the importance of lipid signaling in neuronal trash elimination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Chemistry, Medicinal
You-Jung Ha, Yong Seok Choi, Ye Rim Oh, Eun Ha Kang, Gilson Khang, Yong-Beom Park, Yun Jong Lee
Summary: The study demonstrates that Fucoxanthin (FX) regulates osteoclast differentiation and bone resorption ability by decreasing the activation of mitogen-activated protein kinases and increasing the nuclear translocation of phosphonuclear factor erythroid 2-related factor 2. By inhibiting specific signaling pathways, FX may have potential therapeutic effects on bone diseases like osteoporosis and rheumatoid arthritis.
Article
Biology
Payam E. Farahani, Xiaoyu Yang, Emily Mesev, Kaylan A. Fomby, Ellen H. Brumbaugh-Reed, Caleb J. Bashor, Celeste M. Nelson, Jared E. Toettcher
Summary: pYtags is a modular approach for monitoring the activity of a specific RTK by live-cell microscopy. It enables monitoring of a specific RTK on different time and space scales with high specificity. Using pYtag biosensors, signaling dynamics can be quantitatively characterized with different activating ligands. The specificity and modularity of pYtags open the door to robust biosensors of multiple tyrosine kinases.
Article
Cell Biology
Armando Jesus Perez-Diaz, Beatriz Vazquez-Marin, Jero Vicente-Soler, Francisco Prieto-Ruiz, Teresa Soto, Alejandro Franco, Jose Cansado, Marisa Madrid
Summary: The autophagy pathway in yeast is regulated differently in response to glucose limitation, indicating evolutionary variations in the signaling pathways that govern autophagy during glucose shortage or starvation.
Review
Medicine, Research & Experimental
Ajay Modi, Priyanka Pandey, Ankit Uniyal, Deepak Chouhan, Somesh Agrawal, Meghana Allani, Anurag Kumar Singh, Sonu Kumar, Vinod Tiwari
Summary: Chronic pain is a common and debilitating condition with a huge burden on society and the economy. Current drugs in clinics are not effective and have severe side effects, leading to treatment withdrawal and poor quality of life. Finding new therapies with minimal side effects is a high research priority.
Article
Biochemistry & Molecular Biology
Nora Dieguez-Martinez, Sergio Espinosa-Gil, Guillermo Yoldi, Elisabet Megias-Roda, Idoia Bolinaga-Ayala, Maria Vinas-Casas, Gokhan Gorgisen, Ines Domingo-Orti, Hector Perez-Montoyo, Jose R. Bayascas, Eva Colas, Xavier Dolcet, Jose M. Lizcano
Summary: Endometrial cancer is the most common gynecologic cancer in developed countries, and its overall survival rate is poor. This study suggests that inhibiting the ERK5/NF-kB pathway can suppress cell proliferation and survival in endometrial cancer and enhance the efficacy of chemotherapy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaorong Liu, Pui Ki Tsang, Matthew B. Soellner, Charles L. Brooks
Summary: Multisite lambda-dynamics (MS lambda D) is a powerful tool in structure-based drug design, allowing for the calculation of relative free energies of binding for a large number of molecules with multiple functional groups. In this study, MS lambda D was used to calculate the binding free energies of 1296 inhibitors to TSSK1B, a target for male contraception. The method proved to be computationally efficient compared to traditional free energy methods, and the results helped establish a quantitative structure-activity relationship (QSAR) for this set of molecules.
Article
Chemistry, Organic
Yi Wolf Zhang, Nan Zheng, Danny Hung-Chieh Chou
Summary: Phage display is a useful tool for discovering therapeutic antibodies and proteins. This study developed a new method for displaying insulin-like peptides on M13 phage, addressing the challenges posed by insulin's complex structure. The method has potential for displaying other structurally complex proteins on phage.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)