Review
Biochemistry & Molecular Biology
Morgana K. Kellogg, Sarah C. Miller, Elena B. Tikhonova, Andrey L. Karamyshev
Summary: Signal recognition particle (SRP) is an RNA and protein complex found across all domains of life, with varying compositions and functions in different organisms. Its main function involves targeting proteins to the endoplasmic reticulum and protecting the mRNA of secretory proteins from degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Monika I. Linder, Yoko Mizoguchi, Sebastian Hesse, Gergely Csaba, Megumi Tatematsu, Marcin Lyszkiewicz, Natalia Zietara, Tim Jeske, Maximilian Hastreiter, Meino Rohlfs, Yanshan Liu, Piotr Grabowski, Kaarin Ahomaa, Daniela Maier-Begandt, Marko Schwestka, Vahid Pazhakh, Abdulsalam I. Isiaku, Brenda Briones Miranda, Piers Blombery, Megumu K. Saito, Ejona Rusha, Zahra Alizadeh, Zahra Pourpak, Masao Kobayashi, Nima Rezaei, Ekrem Unal, Fabian Hauck, Micha Drukker, Barbara Walzog, Juri Rappsilber, Ralf Zimmer, Graham J. Lieschke, Christoph Klein
Summary: This study discovered two novel genetic defects in SRPRA and SRP19 genes, which are involved in the differentiation of neutrophil granulocytes. Proteome analysis of neutrophil granulocytes from patients with variants in SRP genes revealed global and specific proteome aberrations. In vitro and in vivo experiments confirmed the crucial role of SRP-dependent protein processing, intracellular trafficking, and homeostasis in neutrophil granulocyte differentiation.
Article
Plant Sciences
Shuiling Ji, Bernhard Grimm, Peng Wang
Summary: This study reveals the distinct roles of cpSRP43 and cpSRP54 in optimizing the function of the major PORB isoform in Arabidopsis, with the former stabilizing the enzyme and providing appropriate amounts of PORB and the latter enhancing its binding to the thylakoid membrane to ensure adequate metabolic flux.
Editorial Material
Biochemistry & Molecular Biology
Oliver Weichenrieder
Summary: In this study, Juaire et al. investigated disease-associated variants of the SRP54 GTPase using X-ray crystallography, biophysical tools, and cell-based assays. They demonstrated that defects in SRP-mediated protein secretion can explain phenotypes of severe neutropenia with Shwachman-Diamond-syndrome-like symptoms.
Article
Biochemistry & Molecular Biology
Jan Pyrih, Tomas Panek, Ignacio Miguel Durante, Vendula Raskova, Kristyna Cimrhanzlova, Eva Kriegova, Anastasios D. Tsaousis, Marek Elias, Julius Lukes
Summary: This study reveals the presence of homologs of bacterial Ffh and FtsY proteins in various plastid-lacking unicellular eukaryotes, suggesting they constitute parts of an ancestral mitochondrial signal peptide-based protein-targeting system inherited from the last eukaryotic common ancestor. This system appears to have been lost from the majority of extant eukaryotes, with implications for protein targeting in different organisms.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Stacy A. Anderson, Manasa B. Satyanarayan, Ryan L. Wessendorf, Yan Lu, Donna E. Fernandez
Summary: The study shows that the chloroplast homolog GET3B of Arabidopsis thaliana structurally resembles cytosolic Get3 proteins and selectively binds to a thylakoid-localized TA protein. Through genetic interactions and changes in protein abundance, it is suggested that GET3B primarily directs proteins to the thylakoids in a manner similar to cytosolic Get3 proteins.
Review
Biochemistry & Molecular Biology
Michael Fish, Delaney Nash, Alexandru German, Alyssa Overton, Masoud Jelokhani-Niaraki, Simon D. X. Chuong, Matthew D. Smith
Summary: Plastids, specialized organelles in plant cells, originated from ancient cyanobacteria. As genes encoding plastid proteins were transferred to the nuclear genome during evolution, eukaryotic cells developed targeting pathways and protein machinery to direct these proteins back to the plastids. Chloroplasts, the most well-studied plastids, play essential roles in photosynthesis and metabolism. Understanding the pathways and regulation of protein targeting is vital for chloroplast biogenesis and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Martin R. Pool
Summary: The endoplasmic reticulum serves as the entry point for the secretory pathway, responsible for targeting proteins to their appropriate destinations. This targeting process involves the recognition of specific targeting signals and their subsequent delivery to the translocases present at the ER membrane. Targeting is typically mediated by cleavable N-terminal hydrophobic signal sequences or internal membrane anchor sequences, and the location and features of the targeting sequence determine which targeting pathway substrates are used.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Liuqun Zhao, Yanyan Cui, Gang Fu, Zixiang Xu, Xiaoping Liao, Dawei Zhang
Summary: The signal recognition particle (SRP) is not essential for inner membrane protein (IMP) targeting in E. coli, as suppressor screening identified translation initiation factors that compensate for SRP loss. The time delay in translation initiation plays a critical role in IMP targeting by extending the window for ribosomes to reach the inner membrane.
Article
Biochemistry & Molecular Biology
Jing Yang, Tetsuya Hirata, Yi-Shi Liu, Xin-Yu Guo, Xiao-Dong Gao, Taroh Kinoshita, Morihisa Fujita
Summary: Many GPI-APs like CD59, CD55, and CD109 utilize the hSND2-dependent ER targeting machinery, with signal recognition particle receptors cooperating to facilitate this process. The hydrophobicity of the C-terminal GPI attachment signal serves as a determinant of hSND2 dependency in ER targeting of GPI-APs.
Article
Biochemistry & Molecular Biology
Keven D. Juaire, Karine Lapouge, Matthias M. M. Becker, Irina Kotova, Michelle Michelhans, Raphael Carapito, Klemens Wild, Seiamak Bahram, Irmgard Sinning
Summary: Mutations in SRP54 have been found to cause a severe congenital neutropenia with symptoms overlapping with Shwachman-Diamond syndrome. These mutations result in critical protein destabilization, affecting the entire SRP pathway's function.
Article
Biochemistry & Molecular Biology
Joori Park, Jeeyoon Chang, Hyun Jung Hwang, Kwon Jeong, Hyuk-Joon Lee, Hongseok Ha, Yeonkyoung Park, Chunghun Lim, Jae-Sung Woo, Yoon Ki Kim
Summary: This study identified a surveillance pathway in which pioneer translation ensures proper targeting of endoplasmic reticulum and mitochondrial proteins, preventing abnormal protein folding and cytosolic stress response.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biology
Samuel James Watson, Na Li, Yiting Ye, Feijie Wu, Qihua Ling, R. Paul Jarvis
Summary: The stability of the TOC complex, a key protein channel for chloroplast import, can be regulated by the ubiquitin-dependent pathway as well as the SUMO system. Inhibiting SUMO tagging results in increased TOC protein levels and improved chloroplast development, potentially impacting the growth performance of crops.
Article
Microbiology
Eva Pross, Andreas Kuhn
Summary: The study found that the C-tailed protein SciP has a unique membrane targeting mechanism, utilizing two SRP signal sequences to facilitate membrane insertion during synthesis, unlike the typical posttranslational insertion. This mechanism may enhance the efficiency of SciP's membrane targeting process.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Cell Biology
Andrea Tirincsi, Sarah O'Keefe, Duy Nguyen, Mark Sicking, Johanna Dudek, Friedrich Forster, Martin Jung, Drazena Hadzibeganovic, Volkhard Helms, Stephen High, Richard Zimmermann, Sven Lang
Summary: Importing proteins into the endoplasmic reticulum is crucial for approximately 30% of the human proteome, involving precursor protein targeting and insertion or translocation across the ER membrane with the help of signals in precursor polypeptides. Little is known about the SRP-independent/SND pathway, and our study aimed to identify additional components and characterize the client spectrum of this pathway, finding that SND clients are predominantly membrane proteins.
Article
Biochemistry & Molecular Biology
Harry B. Gristick, Michael E. Rome, Justin W. Chartron, Meera Rao, Sonja Hess, Shu-ou Shan, William M. Clemons
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Correction
Neurosciences
Nripesh Dhungel, Simona Eleuteri, Ling-bo Li, Nicholas J. Kramer, Justin W. Chartron, Brian Spencer, Kori Kosberg, Jerel Adam Fields, Klodjan Stafa, Anthony Adame, Hilal Lashuel, Judith Frydman, Kang Shen, Eliezer Masliah, Aaron D. Gitler
Article
Neurosciences
Nripesh Dhungel, Simona Eleuteri, Ling-bo Li, Nicholas J. Kramer, Justin W. Chartron, Brian Spencer, Kori Kosberg, Jerel Adam Fields, Klodjan Stafa, Anthony Adame, Hilal Lashuel, Judith Frydman, Kang Shen, Eliezer Masliah, Aaron D. Gitler
Article
Multidisciplinary Sciences
Justin W. Chartron, Katherine C. L. Hunt, Judith Frydman
Article
Biochemistry & Molecular Biology
Justin W. Chartron, William M. Clemons, Christian J. M. Suloway
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Justin W. Chartron, Grecia M. Gonzalez, William M. Clemons
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Justin W. Chartron, David G. VanderVelde, Meera Rao, William M. Clemons
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Sebastian Pechmann, Justin W. Chartron, Judith Frydman
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Harry B. Gristick, Meera Rao, Justin W. Chartron, Michael E. Rome, Shu-ou Shan, William M. Clemons
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Christian J. M. Suloway, Justin W. Chartron, Ma'ayan Zaslaver, William M. Clemons
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2009)
Article
Multidisciplinary Sciences
Justin W. Chartron, Christian J. M. Suloway, Ma'ayan Zaslaver, William M. Clemons
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Multidisciplinary Sciences
Jee-Young Mock, Justin William Chartron, Ma'ayan Zaslaver, Yue Xu, Yihong Ye, William Melvon Clemons
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Cell Biology
Justin W. Chartron, David G. VanderVelde, William M. Clemons
Article
Biochemistry & Molecular Biology
Sarah Thorwall, Cory Schwartz, Justin W. Chartron, Ian Wheeldon
NATURE CHEMICAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)