Review
Cell Biology
Linbin Zhou, Danny Siu-Chun Ng, Jason C. Yam, Li Jia Chen, Clement C. Tham, Chi Pui Pang, Wai Kit Chu
Summary: This review summarizes recent findings on the impact of post-translational modifications (PTMs) on the functions of retinoblastoma protein (pRb). PTMs on pRb play crucial roles in cell cycle regulation, cell survival, and differentiation, and altered PTMs can lead to anomalies in cell proliferation and tumorigenesis. Understanding the post-translational modulation of pRb could facilitate the development of novel therapeutic strategies for various human diseases.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Clinical Neurology
Carolina Alquezar, Shruti Arya, Aimee W. Kao
Summary: Post-translational modifications play a crucial role in regulating tau protein function, including phosphorylation and acetylation. While these modifications may regulate protein function under normal conditions, they can be hijacked in disease states. Limited understanding exists on how these modifications impact tau protein, but studies indicate their critical role in localization, interactions, and other aspects.
FRONTIERS IN NEUROLOGY
(2021)
Article
Virology
Yaqin Wang, Tao Hu, Yuting He, Chenlu Su, Zhanqi Wang, Xueping Zhou
Summary: N-terminal acetylation (N-acetylation) is a common protein modification in animals and plants, but little is known about its role in viral proteins. In this study, the Thr-2 residue in the I3C1 protein encoded by the betasatellite of tomato yellow leaf curl China virus (TYLCCNB-I3C1) was identified as a novel N-acetylation site. The study revealed that N-acetylation of TYLCCNB-I3C1 is critical for its nuclear self-interaction and viral pathogenesis. Removal of N-acetylation attenuated symptoms induced by tomato yellow leaf curl China virus and accelerated degradation of TYLCCNB-I3C1 through the ubiquitin-proteasome system, demonstrating an antagonistic crosstalk between N-acetylation and ubiquitination in this viral protein.
Review
Neurosciences
Jiurong Cheng, Yingdong Deng, Jun Zhou
Summary: Chronic pain, including neuropathic pain and inflammatory pain, is a significant public health issue with elusive underlying mechanisms. The roles of ubiquitin in various physiological and pathological conditions suggest its potential as therapeutic targets. Dysfunctions in the ubiquitin system components during chronic pain are rapidly being discovered, providing insights into potential molecular mechanisms of chronic pain and contributing to the development of novel analgesics.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Review
Geriatrics & Gerontology
Xiaodie Gao, Qiang Chen, Hua Yao, Jie Tan, Zheng Liu, Yan Zhou, Zhenyou Zou
Summary: Alzheimer's disease is a complex neurodegenerative disease with unknown causes and pathological changes. Targeted drug studies on the typical pathological phenomenon in AD have had high failure rates, and the existing drugs have low efficacy and significant side effects, leading to an urgent need for new treatment strategies. Increasing studies have shown epigenetic changes in AD, providing a new avenue for medical researchers worldwide.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Review
Oncology
Jordan M. Broekhuis, Benjamin C. James, Richard D. Cummings, Per-Olof Hasselgren
Summary: Alterations to protein function following production can impact tumor development in thyroid cancer. Research on post-translational modifications may improve diagnostics, treatment, and development of novel therapies for thyroid cancer.
Review
Biochemistry & Molecular Biology
Siyi Zhang, Tingting Zhou, Zhuo Wang, Fei Yi, Chunlu Li, Wendong Guo, Hongde Xu, Hongyan Cui, Xiang Dong, Jingwei Liu, Xiaoyu Song, Liu Cao
Summary: Faithful DNA replication is crucial for cell survival and inheritance, but damage can lead to replication stalling and DNA lesions. Post-translational modifications of PCNA regulate DNA synthesis and damage tolerance to maintain genomic stability. Dysregulation of PCNA PTMs can result in DNA breaks, affecting cell response and causing mutations.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ga Hyun Park, Joon Hyung Park, Kwang Chul Chung
Summary: Parkinson's disease is caused by the loss of dopaminergic neurons due to mitochondrial dysfunction, leading to toxic oligomer formation of alpha-synuclein. Familial PD-related proteins such as alpha-synuclein play a role in mitophagy, with defective changes in mitochondrial dynamics inducing the formation of toxic protein aggregates in PD. The genes involved in mitochondrial quality control and UPS are closely related to PD pathogenesis and may provide valuable therapeutic clues for management strategies.
Review
Biochemistry & Molecular Biology
Anmol Sharma, Heena Khan, Thakur Gurjeet Singh, Amarjot Kaur Grewal, Agnieszka Najda, Malgorzata Kawecka-Radomska, Mohamed Kamel, Ahmed E. Altyar, Mohamed M. Abdel-Daim
Summary: The ubiquitin-proteasome pathway is crucial in regulating various biological functions and plays a significant role in tumor development. Inhibiting specific enzymes within this pathway can be an effective treatment for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Olga Buneeva, Alexei Medvedev
Summary: Ubiquitination is a major post-translational modification of proteins, and atypical ubiquitination plays a crucial role in the development of Parkinson's disease, targeting proteins involved in the disease mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yekatarina Gadasheva, Alexander Nolze, Claudia Grossmann
Summary: Cardiovascular aging leads to increased arterial stiffness and left ventricular hypertrophy, with the renin-angiotensin-aldosterone system playing a key role in regulating electrolyte balance and blood pressure. The mineralocorticoid receptor can undergo enzymatic and non-enzymatic modifications, impacting its activity independently of its ligand aldosterone. These modifications may have implications for cardiovascular aging and diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Xiang Chen, Zaw Min Htet, Erika Lopez-Alfonzo, Andreas Martin, Kylie J. Walters
Summary: The 26S proteasome is responsible for regulated proteolysis in eukaryotic cells, with substrates targeted to it through post-translational modification with ubiquitin. Deubiquitinating enzymes (DUBs) play a crucial role in catalyzing ubiquitin chain hydrolysis, coupling deubiquitination to substrate unfolding and degradation. This intricate machinery of proteasomes and ubiquitin-modified substrates remains an active area of investigation, with potential for therapeutic targeting.
Review
Biochemistry & Molecular Biology
Min Qiu, Jimei Chen, Xiaohong Li, Jian Zhuang
Summary: Cardiovascular diseases (CVDs) are a major social problem worldwide, and oxidative stress plays a vital role in CVDs. The ubiquitin-proteasome system (UPS) is associated with oxidative stress and affects the development of CVDs. This review provides a comprehensive analysis of the roles of UPS and oxidative stress in CVDs, suggesting new targets for the treatment and prevention of CVDs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Yi Xiong, Chao Yu, Qianting Zhang
Summary: This review discusses the functions of ubiquitin proteasome system (UPS) components in mammalian spermatogenesis, including the composition of proteasome isoforms at each stage, the specificity and associated degradation events of each isoform, E3 ubiquitin ligases mediating protein ubiquitination, and the deubiquitinases involved in spermatogenesis and male fertility. Exploring the UPS machineries in spermatogenesis provides insights into protein dynamics during male germ cell production and sheds light on the etiology and pathogenesis of human male infertility.
Review
Biochemistry & Molecular Biology
Jessica Proulx, Kathleen Borgmann, In-Woo Park
Summary: Viruses encode viral DUBs to disrupt cellular Ub-dependent processes, evade host immune response, and enhance virus replication and proliferation within infected cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chen-Ching Yuan, Katarzyna Kazmierczak, Jingsheng Liang, Zhiqun Zhou, Sunil Yadav, Aldrin V. Gomes, Thomas C. Irving, Danuta Szczesna-Cordary
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemical Research Methods
Hannah Sander, Samantha Wallace, Rachel Plouse, Shuchita Tiwari, Aldrin Gomes
ANALYTICAL BIOCHEMISTRY
(2019)
Article
Biochemical Research Methods
Manish Mishra, Shuchita Tiwari, Anita Gunaseelan, Dongyang Li, Bruce D. Hammock, Aldrin V. Gomes
Article
Multidisciplinary Sciences
Darko Bosnakovski, Meiricris T. da Silva, Sithara T. Sunny, Elizabeth T. Ener, Erik A. Toso, Ce Yuan, Ziyou Cui, Michael A. Walters, Ajit Jadhav, Michael Kyba
Article
Multidisciplinary Sciences
Shuchita Tiwari, Manish Mishra, Michelle R. Salemi, Brett S. Phinney, Joanne L. Newens, Aldrin Gomes
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Medicinal
Haoqing Chen, Ziyou Cui, Leila Hejazi, Lihua Yao, Scott J. Walmsley, Carmelo J. Rizzo, Robert J. Turesky
CHEMICAL RESEARCH IN TOXICOLOGY
(2020)
Article
Multidisciplinary Sciences
Shuchita Tiwari, Jun Yang, Christophe Morisseau, Blythe Durbin-Johnson, Bruce D. Hammock, Aldrin Gomes
Summary: The study revealed that ibuprofen disrupts not only the COX pathway, but also the CYP450 and lipoxygenase pathways in male mice, generating sex-related differences in biologically active oxylipins. Increased sEH activity after ibuprofen treatment is likely to be one of the mechanisms by which the liver reduces the higher levels of EpODEs and EpETrEs.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Oladapo O. Yeku, Thapi Dharma Rao, Ian Laster, Artem Kononenko, Terence J. Purdon, Pei Wang, Ziyou Cui, Hong Liu, Renier J. Brentjens, David Spriggs
Summary: The study demonstrates the feasibility and efficacy of MUC16(ecto)-specific BiTEDs in delaying tumor progression and improving survival rates in ovarian cancer, with enhanced effect when combined with anti-VEGF therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Rasheed O. Sule, Liam Condon, Aldrin V. Gomes
Summary: Pesticides are important chemicals or biological agents used to deter or kill pests. While they are effective in reducing pests and increasing crop growth, they also have potential toxic effects on humans and wildlife, such as increased risk of cardiovascular disease, cancer, and birth defects. Pesticides induce oxidative stress, associated with various diseases, through reactive oxygen and nitrogen species. Limited in vitro studies suggest that exogenous antioxidants may mitigate the harmful effects of pesticides.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Henning T. Langer, Agata A. Mossakowski, Rasheed Sule, Aldrin Gomes, Keith Baar
Summary: Overexpression of a dominant-negative p53 mutant in skeletal muscle leads to decreased muscle mass and fiber size, resulting in tissue damage, metabolic changes, and imbalance between muscle protein synthesis and degradation.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Chang Liu, Hong Liu, Moumita Dasgupta, Lance M. Hellman, Xiaogang Zhang, Kai Qu, Hui Xue, Yun Wang, Fenling Fan, Qi Chang, Duo Yu, Linhu Ge, Yu Zhang, Ziyou Cui, Pengbo Zhang, Bradley Heller, Hongbing Zhang, Bingyin Shi, Brian M. Baker, Cheng Liu
Summary: This article describes a novel TCRm-based T cell therapy that shows specificity and safety for hepatocellular carcinoma (HCC). The therapy exhibits potent activity against AFP-positive cancer cell lines in both in vitro and in vivo models without affecting AFP-negative cells. In a human safety assessment, no significant adverse events were observed, and evidence of efficacy was seen. Remarkably, one metastatic HCC patient achieved complete remission after nine months and qualified for a liver transplant.
SCIENTIFIC REPORTS
(2022)
Review
Biochemical Research Methods
Rasheed Sule, Gabriela Rivera, Aldrin Gomes
Summary: Western blotting is a powerful technique for detecting or semiquantifying individual proteins from complex mixtures of cell or tissue extracts. This article provides a comprehensive overview of the history, theory, protocol, and applications of western blotting. It also discusses lesser known but significant problems in the field and troubleshooting techniques. This work serves as a primer and guide for new western blotting researchers and those seeking a better understanding of the technique or improved results.
Meeting Abstract
Biochemistry & Molecular Biology
Aldrin Gomes, Shuchita Tiwari, Rasheed Sule
Meeting Abstract
Biochemistry & Molecular Biology
Shuchita Tiwari, Aldrin V. Gomes
Article
Medicine, Research & Experimental
Jan L. A. Voskuil, Anita Bandrowski, C. Glenn Begley, Andrew R. M. Bradbury, Andrew D. Chalmers, Aldrin V. Gomes, Travis Hardcastle, Fridtjof Lund-Johansen, Andreas Plueckthun, Giovanna Roncador, Alejandra Solache, Michael J. Taussig, James S. Trimmer, Cecilia Williams, Simon L. Goodman
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)