Article
Biochemistry & Molecular Biology
Miriam M. Cortese-Krott, Tatsiana Suvorava, Francesca Leo, Sophia K. Heuser, Anthea LoBue, Junjie Li, Stefanie Becher, Rebekka Schneckmann, Tanu Srivrastava, Ralf Erkens, Georg Wolff, Joachim P. Schmitt, Maria Grandoch, Jon O. Lundberg, John Pernow, Brant E. Isakson, Eddie Weitzberg, Malte Kelm
Summary: This study reveals that red blood cell endothelial nitric oxide synthase (RBC eNOS) plays a role in limiting infarct size in acute myocardial infarction (AMI) and regulates coronary blood flow and cardiac function. These findings suggest that RBC eNOS signaling may represent a novel target for interventions in ischemia/reperfusion after myocardial infarction.
Article
Biochemistry & Molecular Biology
Daniele Mancardi, Pasquale Pagliaro, Lisa A. Ridnour, Carlo G. Tocchetti, Katrina Miranda, Magdalena Juhaszova, Steven J. Sollott, David A. Wink, Nazareno Paolocci
Summary: Nitroxyl (HNO), the one electron-reduction product of nitric oxide (NO), can limit ischemia-reperfusion (I/R) injury by inhibiting the opening of mitochondrial permeability transition pore (mPTP). This study provides insights into the mechanisms of HNO-mediated cardioprotection.
Article
Cardiac & Cardiovascular Systems
Doria Boulghobra, Mathilde Dubois, Beatrice Alpha-Bazin, Florence Coste, Maxime Olmos, Sandrine Gayrard, Isabelle Bornard, Gregory Meyer, Jean-Charles Gaillard, Jean Armengaud, Cyril Reboul
Summary: Exercise training increases the activation of eNOS and its localization in mitochondria, leading to enhanced nitric oxide production, which may represent a key mechanism of exercise-induced cardioprotection.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Cell Biology
Kenichiro Suno, Yasushige Shingu, Satoru Wakasa
Summary: This study evaluated the effects of trehalose preconditioning on cardiac and coronary endothelial function after ischemia-reperfusion injury. It was found that trehalose preconditioning improved heart function, reduced edema, oxidative stress, and apoptosis, and increased eNOS dimerization ratio, indicating its protective effects on cardiac and coronary endothelial function. These effects were mediated through the eNOS signaling pathway.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Fang Yuan, Cuiling Zhang, Xianzhu Luo, Xiaokun Shen, Yuezhong Xian
Summary: ATP stimulus-responsive tetrahedral DNA-gated fluorescent covalent organic frameworks (COFs) were developed for estradiol (E2) delivery and controllable release. The fluorescent COFs with an efficient E2 loading showed great potential against myocardial ischemia and reperfusion injury.
CHEMICAL COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Shuhan Bu, Hien C. Nguyen, Sepideh Nikfarjam, David C. R. Michels, Berk Rasheed, Sauraish Maheshkumar, Shweta Singh, Krishna K. Singh
Summary: The endothelium maintains vascular homeostasis by balancing vasorelaxation and vasoconstriction through regulating Nitric Oxide (NO) availability. Endothelial nitric oxide synthase (eNOS) is involved in this process and regulates endothelial function such as cell proliferation, migration, and tube formation. Inhibition of eNOS through genetic or pharmacologic methods has shown differential effects on endothelial cell behavior, suggesting a more complex mechanism behind eNOS regulation.
Article
Biology
Yiying Wang, Qiannan Li, Zhiyang Zhang, Kai Peng, Dai-Min Zhang, Qianlu Yang, Anthony G. Passerini, Scott I. Simon, ChongXiu Sun
Summary: Inhibition of endothelium-specific mTOR complexes impairs endothelial-dependent vasodilation and reduces nitric oxide bioavailability. This is due to suppressed eNOS gene expression and increased reactive oxygen species production. Restoring KLF2 expression or suppressing Nox2 can reverse the impaired vasodilation in mice with inhibited mTOR complexes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Anees Ahmed Syed, Mohammed Shafiq, Mohammad Irshad Reza, Pragya Bharati, Athar Husain, Pragati Singh, Kashif Hanif, Jiaur R. Gayen
Summary: The chronic treatment of Cissus quadrangularis extract improved endothelium-dependent relaxation in spontaneously hypertensive rats by activating eNOS and inhibiting ROS production and inflammation, leading to antihypertensive effects.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2022)
Article
Biochemistry & Molecular Biology
Sheng-Qing Gao, Jia-Jun Shi, Shu-Hao Miao, Tao Li, Chao-Chao Gao, Yan-Ling Han, Jia-Yin Qiu, Yun-Song Zhuang, Meng-Liang Zhou
Summary: This study investigated the contribution of the interaction between endothelial NOX4 and DHFR to eNOS uncoupling after SAH. Inhibiting endothelial NOX4 and increasing endothelial DHFR levels alleviated eNOS uncoupling and improved neurological function after SAH.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Yiru Guo, Qianhong Li, Yu-Ting Xuan, Wen-Jian Wu, Wei Tan, Jan Slezak, Xiaoping Zhu, Alex Tomlin, Roberto Bolli
Summary: This study demonstrates that short-term, mild exercise induces delayed cardioprotective effects against myocardial infarction, dependent on the activation of eNOS, eNOS-derived NO generation, and subsequent PKC epsilon activation during preconditioning. Furthermore, the protection 24 hours after exercise is mediated by iNOS activity.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Neurosciences
David Perez-Boyero, Carlos Hernandez-Perez, Jorge Valero, Valeria Lorena Cabedo, Jose Ramon Alonso, David Diaz, Eduardo Weruaga
Summary: The main olfactory bulb (MOB) is a neural structure that processes olfactory information. Nitric oxide (NO), produced mainly by neuronal nitric oxide synthase (nNOS), plays a crucial role in the plasticity and functioning of the MOB. The absence of nNOS expression in nNOS-KO mice leads to a reduced olfactory capacity, while an increase in the expression of endothelial nitric oxide synthase (eNOS) is observed. However, the level of NO generated in the MOB does not appear to change significantly. These findings suggest that nNOS is essential for the proper functioning of the olfactory system.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Yi-Lin Chiu, Chung-Yueh Hsieh, Guo-Shiang Tsung, Lian-Shan Wu, Cheng-Chung Cheng, Tsung-Neng Tsai
Summary: Cilostazol exerts antithrombotic and vasculoprotective effects in endothelial cells by activating KLF2 and SIRT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Francesca Leo, Tatsiana Suvorava, Sophia K. Heuser, Junjie Li, Anthea LoBue, Frederik Barbarino, Eugenia Piragine, Rebekka Schneckmann, Beate Hutzler, Miranda E. Good, Bernadette O. Fernandez, Lukas Vornholz, Stephen Rogers, Allan Doctor, Maria Grandoch, Johannes Stegbauer, Eddie Weitzberg, Martin Feelisch, Jon O. Lundberg, Brant E. Isakson, Malte Kelm, Miriam M. Cortese-Krott
Summary: This study demonstrates that eNOS in ECs and RBCs independently contribute to blood pressure homeostasis. EC eNOS KO mice exhibited significantly impaired aortic dilatory responses to acetylcholine, loss of flow-mediated dilation, and increased blood pressure. On the other hand, RBC eNOS KO mice showed no alterations in acetylcholine-mediated dilation or flow-mediated dilation but were hypertensive.
Article
Endocrinology & Metabolism
Lei Zhao, Cheng-Lin Zhang, Lei He, Qinghua Chen, Limei Liu, Lijing Kang, Jian Liu, Jiang-Yun Luo, Lingshan Gou, Dan Qu, Wencong Song, Chi Wai Lau, Ho Ko, Vincent C. T. Mok, Xiao Yu Tian, Li Wang, Yu Huang
Summary: Monomerization and uncoupling of eNOS play crucial roles in mediating vascular dysfunction in diabetes. This study reveals that impaired autophagic flux leads to eNOS monomerization and reduced nitric oxide bioavailability in diabetic mice. Restoring autophagic flux improves endothelial cell oxidative stress, promotes eNOS dimerization, and enhances endothelium-dependent relaxations in diabetic mice.
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)