期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 52, 期 1, 页码 32-42出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2011.09.002
关键词
Calcium transients; Estrogen; Excitation-contraction coupling; Gender; Sex differences
资金
- Canadian Institutes for Health Research
- Heart and Stroke Foundation of New Brunswick
- Nova Scotia Health Research Foundation
- Heart and Stroke Foundation of Nova Scotia
This study compared Ca2+ homeostasis in ventricular myocytes from 8-month-old female C57BL/6J mice that had either a bilateral ovariectomy (OVX) or a sham surgery at 3 weeks of age. Cells were loaded with fura-2 and field-stimulated or voltage-clamped with steps to membrane potentials between -40 and +80 mV (37 degrees C). Peak Ca2+ transients increased by two-fold in OVX myocytes when compared to sham, and Ca2+ transient rates of rise and decay were faster in OVX cells. In contrast, Ca2+ current densities were similar in sham and OVX cells. Sarcoplasmic reticulum (SR) Ca2+ content, assessed by caffeine, also was higher in OVX compared to sham cells (111.7 +/- 11.9 vs. 61.2 +/- 10.4 nM; p<0.05). Furthermore, the gain of Ca2+ release (Ca2+ release/Ca2+ current) was significantly greater in OVX than in sham cells (16.3 +/- 2.5 vs. 7.7 +/- 2.0 nM/pApF(-1) at 0 my; p<0.05). As changes in unitary Ca2+ release might account for the increased gain in OVX cells, spontaneous Ca2+ sparks were compared in fluo-4-loaded myocytes (37 degrees C). Spark frequency was higher in OVX cells than in sham cells. In addition, spark amplitudes were greater in OVX than in sham myocytes (Delta 6F/F-0=0.379 +/- 0.006 vs. 0342 +/- 0.006; p<0.05). However, spark widths and time courses were similar in the two groups. These data suggest that the size of individual SR Ca2+ release units is larger and the SR Ca2+ content is greater in myocytes of OVX mice, producing augmented gain and SR Ca2+ release. These observations show that OVX disrupts intracellular Ca2+ homeostasis and suggest that sex steroid hormones modulate unitary Ca2+ release in individual cardiac myocytes. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据