Article
Biochemistry & Molecular Biology
Yuval Shemer, Lucy N. Mekies, Ronen Ben Jehuda, Polina Baskin, Rita Shulman, Binyamin Eisen, Danielle Regev, Eloisa Arbustini, Brenda Gerull, Mihaela Gherghiceanu, Eyal Gottlieb, Michael Arad, Ofer Binah
Summary: LMNA-related dilated cardiomyopathy is an inherited heart disease characterized by left ventricular enlargement and impaired systolic function caused by mutations in the LMNA gene, leading to abnormalities in cardiac physiology and gene expression. Studying induced pluripotent stem cell-derived cardiomyocytes from patients can provide insights into potential therapeutic strategies for the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Shi Hua Tan, Sze Jie Loo, Yu Gao, Zhong Hao Tao, Li Ping Su, Chen Xu Wang, Sophia L. Zhang, Yong Hui Mu, Ying Hua Cui, Desiree Abdurrachim, Wei Hsin Wang, Janise Lalic, Kheng Choon Lim, Jun Bu, Ru San Tan, Teck Hock Lee, Jianyi Zhang, Lei Ye
Summary: In a porcine model of myocardial infarction, co-treatment with Tb4-microspheres and hiPSC-CMs improved the engraftment and reparative potency of the transplanted hiPSC-CMs, induced vasculogenesis and proliferation of cardiomyocytes and endothelial cells, improved left ventricular systolic function, and reduced infarct size. HiPSC-CM implantation did not increase the incidence of ventricular arrhythmias and did not induce tumorigenesis in the immunosuppressed pigs.
Article
Cell & Tissue Engineering
Xianji Liu, Rui Zhao, Qianqian Ding, Xiaoqiang Yao, Suk Ying Tsang
Summary: TRPC7 regulates the automaticity of mESC-CMs through two mechanisms: by positively regulating the intracellular Ca2+ clock through the regulation of activities of RyR2 and SERCA, and by positively regulating the membrane clock via its influence on NCX activity. The expression level of TRPC7 affects the frequency of Ca2+ transients, local Ca2+ releases, and action potentials.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Weihua Bian, Wangping Chen, Thanh Nguyen, Yang Zhou, Jianyi Zhang
Summary: The study demonstrates that miR-199a-overexpressing hiPSC-CMs can increase cell proliferation in a heart infarction model, improving cardiac function and fibrosis. While the treatment may lead to lethal arrhythmia, there was no evidence of sudden death in the mouse model. Future investigations on miR-199a-overexpressing hiPSC-CMs in larger animals are supported by these results.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Wahiba Dhahri, Tamilla Sadikov Valdman, Dan Wilkinson, Elizabeth Pereira, Eylul Ceylan, Naaz Andharia, Beiping Qiang, Hassan Masoudpour, Fanny Wulkan, Elya Quesnel, Wenlei Jiang, Shunsuke Funakoshi, Amine Mazine, M. Juliana Gomez-Garcia, Neda Latifi, Yidi Jiang, Ella Huszti, Craig A. Simmons, Gordon Keller, Michael A. Laflamme
Summary: The study showed that culturing hPSC-CMs on PDMS-lined roller bottles and transplanting these cells into a guinea pig model of myocardial infarction resulted in better structural and functional outcomes compared to conventional immature hPSC-CM populations. The PDMS-matured hPSC-CMs exhibited increased cardiac gene expression and more mature properties in vitro, and the intracardiac grafts formed with these cells showed enhanced structure and alignment, better electromechanical integration, and improved contractile function. This study provides practical methods for the scaled generation of mature hPSC-CMs and highlights the importance of using more mature cardiomyocytes for cardiac regeneration.
Article
Genetics & Heredity
Kai Liu, Xiaoping Peng, Liang Luo
Summary: This study demonstrates that miR-322 promotes the differentiation of embryonic stem cells (ESCs) into cardiomyocytes by regulating Celf1 expression. The results show that miR-322 upregulates the expression of cardiac markers NKX-2.5, MLC2V, and alpha-MHC, thereby promoting cardiomyocyte differentiation.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Cardiac & Cardiovascular Systems
Meng Zhao, Yuji Nakada, Yuhua Wei, Weihua Bian, Yuxin Chu, Anton V. Borovjagin, Min Xie, Wuqiang Zhu, Thanh Nguyen, Yang Zhou, Vahid Serpooshan, Gregory P. Walcott, Jianyi Zhang
Summary: The study demonstrated that the enhanced potency of CCND2(OE)CMs promoted myocyte proliferation in both grafts and recipient tissue in a large mammal acute myocardial infarction model. These results suggest that CCND2(OE)CMs transplantation may be a potential therapeutic strategy for the repair of infarcted hearts.
Review
Biotechnology & Applied Microbiology
Yang Xiao, Yihuan Chen, Chunlai Shao, Yaning Wang, Shijun Hu, Wei Lei
Summary: Myocardial infarction is a common cardiovascular disease caused by ischemia leading to cardiomyocyte damage and scar tissue formation. Current research focuses on improving the transplantation efficacy of PSC-CMs to enhance the treatment of myocardial infarction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Gentaro Ikeda, Michelle R. Santoso, Yuko Tada, Albert M. Li, Evgeniya Vaskova, Ji-Hye Jung, Connor O'Brien, Elizabeth Egan, Jiangbin Ye, Phillip C. Yang
Summary: This study demonstrated that extracellular vesicle-mediated transfer of autologous mitochondria can enhance cardiac function by restoring myocardial bioenergetics. The therapy shows potential as a precision therapeutic for mitochondria-related diseases such as heart failure.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2021)
Article
Engineering, Biomedical
Peng Wu, Xiyalatu Sai, Zhetao Li, Xing Ye, Li Jin, Guihuan Liu, Ge Li, Pingzhen Yang, Mingyi Zhao, Shuoji Zhu, Nanbo Liu, Ping Zhu
Summary: Induced pluripotent stem cell-derived cardiomyocytes have significant potential in the treatment of myocardial infarction, but their immaturity hinders their therapeutic efficacy. By synthesizing nano colloidal gelatin with collagen and combining it with a PI3K/AKT/mTOR pathway inhibitor, the maturity, function, and biocompatibility of the cardiomyocytes are improved.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Hua Wang, Wen-Shu Zhao, Lin Xu
Summary: This study investigated the functions of bisphosphonate of zoledronate (Zd) in hypoxia/reoxygenation (H/R) injured human embryonic stem cell-derived cardiomyocytes (hES-CMs). The results showed that low and medium concentrations of Zd did not induce cell death and could protect against H/R-induced apoptosis. Zd also promoted the survival of hES-CMs in an animal model of myocardial infarction.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Jacqueline A. Treat, Ryan Pfeiffer, Hector Barajas-Martinez, Robert J. Goodrow, Corina Bot, Rodolfo J. Haedo, Ronald Knox, Jonathan M. Cordeiro
Summary: Human induced pluripotent stem cell-derived cardiomyocytes are used for genetic models of cardiac diseases. An arrhythmia syndrome consisting of Early Repolarization Syndrome and Short QT Syndrome was reported in a patient with six mutations, with functional alterations examined in patient-derived cells. The study revealed abnormal electrophysiological activity in the patient's cardiomyocytes, suggesting potential genetic causes for the syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yuanxue Gao, Shuang Wu, Jiayue Pan, Kai Zhang, Xiaoyi Li, Yangyang Xu, Chentao Jin, Xiao He, Jingjing Shi, Lijuan Ma, Fujian Wu, Yao Yao, Ping Wang, Qinggang He, Feng Lan, Hong Zhang, Mei Tian
Summary: The study demonstrated the establishment of a CRISPR/Cas9-mediated multimodality reporter gene imaging system that can determine the dynamics and function of TF-hUiCMs in myocardial infarction, which is helpful for investigating the application of stem cell therapy.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2021)
Article
Biochemistry & Molecular Biology
Robert D. Johnson, Ming Lei, John H. Mcvey, Patrizia Camelliti
Summary: Human myofibroblasts alter the electrophysiology and Ca2+ handling of hiPSC-CMs, downregulating mRNAs encoding voltage channels and SERCA2a calcium pump. Interleukin-6 is elevated in the presence of myofibroblasts and can recapitulate their paracrine effects on hiPSC-CMs when directly stimulated. Blocking interleukin-6 reduces the effects of myofibroblasts in the absence of physical contact, while myofibroblast-specific connexin43 knockdown reduces functional changes in contact cocultures when combined with interleukin-6 blockade. This study provides valuable insights into understanding how human myofibroblasts modulate hiPSC-CMs and highlights potential targets for reducing arrhythmic risk in cardiac cell therapy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Alexander J. Hodge, Juming Zhong, Elizabeth A. Lipke
BIOTECHNOLOGY AND BIOENGINEERING
(2016)
Article
Veterinary Sciences
Ashley N. Sharpe, Wen J. Seeto, Randolph L. Winter, Qiao Zhong, Elizabeth A. Lipke, Anne A. Wooldridge
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2016)
Article
Engineering, Biomedical
Petra Kerscher, Irene C. Turnbull, Alexander J. Hodge, Joonyul Kim, Dror Seliktar, Christopher J. Easley, Kevin D. Costa, Elizabeth A. Lipke
Article
Engineering, Biomedical
Shantanu Pradhan, Iman Hassani, Wen J. Seeto, Elizabeth A. Lipke
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2017)
Article
Instruments & Instrumentation
Wen Jun Seeto, Elizabeth Ann Lipke
REVIEW OF SCIENTIFIC INSTRUMENTS
(2016)
Article
Multidisciplinary Sciences
Shantanu Pradhan, Ashley M. Smith, Charles J. Garson, Iman Hassani, Wen J. Seeto, Kapil Pant, Robert D. Arnold, Balabhaskar Prabhakarpandian, Elizabeth A. Lipke
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Multidisciplinary
Wen J. Seeto, Yuan Tian, Shantanu Pradhan, Petra Kerscher, Elizabeth A. Lipke
Article
Biotechnology & Applied Microbiology
Samuel Chang, Ferdous Finklea, Bianca Williams, Hanna Hammons, Alexander Hodge, Samantha Scott, Elizabeth Lipke
BIOTECHNOLOGY PROGRESS
(2020)
Article
Materials Science, Biomaterials
Yuan Tian, Elizabeth A. Lipke
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Engineering, Biomedical
Ferdous B. Finklea, Yuan Tian, Petra Kerscher, Wen J. Seeto, Morgan E. Ellis, Elizabeth A. Lipke
Summary: The study demonstrates successful production of functional cardiac tissue microspheres through direct differentiation of hydrogel encapsulated hiPSCs, with high reproducibility and cell viability. These microspheres support efficient cardiac differentiation and have potential applications in biomanufacturing, drug screening, and regenerative therapies.
Article
Engineering, Biomedical
Iman Hassani, Benjamin Anbiah, Peyton Kuhlers, Nicole L. Habbit, Bulbul Ahmed, Martin J. Heslin, James A. Mobley, Michael W. Greene, Elizabeth A. Lipke
Summary: The development of an in vitro tissue-engineered CRC model using PDX cells provides a cost-effective and high-throughput alternative to the in vivo model. This model demonstrates high batch-to-batch consistency and strong correlation with the originating PDX tumors, making it a valuable tool for studying disease progression and tumorigenic mechanisms.
Article
Materials Science, Biomaterials
Wen J. Seeto, Yuan Tian, Shantanu Pradhan, Dmitriy Minond, Elizabeth A. Lipke
Summary: This study successfully fabricated uniform microspheres encapsulating breast cancer cells using synthetic hybrid hydrogels and microfluidics technology, enabling quantification of cancer cell viability, metabolic activity, morphology, and stiffness over time. The research also demonstrated the translational potential of the microsphere platform in high-throughput drug screening.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Engineering, Biomedical
Yuan Tian, Wen J. Seeto, Mayra A. Paez-Arias, Mariah S. Hahn, Elizabeth A. Lipke
Summary: The aim of this study was to investigate the ability of peptides and peptide combinations to support circulating endothelial colony forming cell (ECFC) rolling and adhesion under shear flow, informing bio-material design in moving toward rapid cardiovascular device endothelialization. The study finds that the performance of REDV-containing peptides in facilitating ECFC dynamic adhesion and capture can be improved by combination with alpha 5fi1 integrin-binding peptides, which support ECFC static adhesion. Moreover, when similar in length, the peptide combinations may have synergistic effects in capturing ECFCs.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Iman Hassani, Benjamin Anbiah, Andrew L. Moore, Peter T. Abraham, Ifeoluwa A. Odeniyi, Nicole L. Habbit, Michael W. Greene, Elizabeth A. Lipke
Summary: An in vitro three-dimensional colorectal cancer tissue model (3D-eCRC) using a natural-synthetic hybrid biomaterial was developed to better mimic the tumor microenvironment and successfully demonstrated differences among different cell lines. Long-term culture of the 3D-eCRC model is important for studying mechanistic differences and drug response.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Engineering, Biomedical
Shantanu Pradhan, Jacob M. Clary, Dror Seliktar, Elizabeth A. Lipke
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)