Article
Chemistry, Multidisciplinary
Baolei Liu, Jiayan Liao, Yiliao Song, Chaohao Chen, Lei Ding, Jie Lu, Jiajia Zhou, Fan Wang
Summary: The study introduces a method for multiplexed super-resolution imaging using lifetime-engineered upconversion nanoparticles, achieving a lateral resolution of 185 nm. By utilizing a deep learning algorithm, more accurate decoding compared to a numeric algorithm is achieved, with decoding accuracies above 93% for a three-channel super-resolution imaging.
NANOSCALE ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Marius Glogger, Christoph Spahn, Joerg Enderlein, Mike Heilemann
Summary: SOFI is a super-resolution microscopy technique that analyzes intensity fluctuations to overcome the diffraction limit, and can circumvent fluorophore photobleaching by using reversible and transient fluorophore labels that bind to a target.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Yi-Fan Fang, Yi-Lan Li, Xiao-Ming Li, Ji-Long Liu
Summary: CTP synthase can form filamentous structures called cytoophidia in cells, which has dynamic and reticular properties and provides space for other components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Spectroscopy
Shawn Yoshida, Lydia Kisley
Summary: The extracellular matrix (ECM) is a crucial biophysical environment involved in physiological processes. Super-resolution fluorescence microscopy offers the potential to investigate local, nanoscale, physicochemical variations in the ECM, providing insights into molecular-level ECM processes and guiding future biomedical research directions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Chemistry, Physical
Oleksii Nevskyi, Dominik Woell
Summary: This review discusses the application of super-resolution fluorescence microscopy techniques in nano- and microparticles hydrogels, known as nano- or microgels. It summarizes the progress in studying the morphology, internal structure, dynamics, and diffusion of microgels. The ability to super-resolve structures in three dimensions has greatly advanced the research field and allowed impressive 3D imaging of deformed microgels.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY
(2023)
Review
Nanoscience & Nanotechnology
A. Femius Koenderink, Roman Tsukanov, Joerg Enderlein, Ignacio Izeddin, Valentina Krachmalnicoff
Summary: This article summarizes the recent advances in the field of nanophotonics that have leveraged SMLM and shows how some concepts commonly used in nanophotonics can benefit the development of new microscopy techniques for biophysics. It introduces the basic concepts of SMLM and the observables that can be measured, linking them with their corresponding physical quantities of interest in biophysics and nanophotonics.
Article
Chemistry, Multidisciplinary
Amandeep Kaur, Liam D. Adair, Sarah R. Ball, Elizabeth J. New, Margaret Sunde
Summary: In this study, the researchers developed a fluorescent amyloid sensor called AmyBlink-1 and used it for super-resolution imaging of amyloid structures. They were able to achieve nanoscale imaging of different types of amyloid fibrils and quantify their morphological features.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Alan M. Szalai, Bruno Siarry, Jeronimo Lukin, Sebastian Giusti, Nicolas Unsain, Alfredo Caceres, Florian Steiner, Philip Tinnefeld, Damian Refojo, Thomas M. Jovin, Fernando D. Stefani
Summary: STED-FRET is a method capable of obtaining super-resolved energy transfer images with higher spatial resolution, more accurate quantification of interactions, and suppression of contributions from noninteracting partners.
Article
Engineering, Electrical & Electronic
Miranda Parisi, Massimiliano Lucidi, Paolo Visca, Gabriella Cincotti
Summary: This article reviews various super-resolution microscopy techniques utilized by microbiologists to investigate bacterial cell morphology and functions. The achieved resolution is discussed, with a focus on fluorophore selection and other critical imaging requirements. Recent applications are described, which have provided novel insights into bacterial cellular structures. The pivotal role of super-resolution microscopy in addressing antimicrobial resistance is emphasized.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Seong Ho Kim, Isaac T. S. Li
Summary: DNA-PAINT achieves super-resolution imaging by transiently binding fluorescently-labelled single-stranded DNA imagers to target ssDNA. However, background fluorescence from imagers limits its performance, resulting in longer image acquisition and potential artifacts. To overcome this, we designed a molecular beacon (MB) as the PAINT imager, which reduces background fluorescence when unbound and becomes fluorogenic upon binding to target DNA. We demonstrated that MB-PAINT achieves similar localization precision as traditional linear imager DNA-PAINT and is ideal for fast super-resolution imaging of molecular tension probes in living cells, eliminating potential artifacts from free-diffusing imagers at the cell-substrate interface.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Ji-Hoon Lee, Joyce Han-Ching Chiu, Nicholas J. J. Ginga, Tasdiq Ahmed, M. D. Thouless, Yifan Liu, Shuichi Takayama
Summary: This study developed a three-layer tunable nanochannel system that enables non-electrophoretic linearization and immobilization of native chromatin. Through careful selection of self-blinking fluorescent dyes and nanochannel design, they achieved super-resolution imaging of the linearized chromatin using direct stochastic optical reconstruction microscopy (dSTORM). This work opens up a new avenue for collecting long-range and high-resolution epigenetic information as well as genetic information.
NANOSCALE HORIZONS
(2023)
Review
Chemistry, Inorganic & Nuclear
Zhi Wang, Yu Zhou, Ruohan Xu, Yanzi Xu, Dongfeng Dang, Qifei Shen, Lingjie Meng, Ben Zhong Tang
Summary: This paper summarizes the important role and application of aggregation-induced emission luminogens (AIEgens) in super-resolution imaging (SRI). By introducing the working principles of AIEgens and SRI techniques, it highlights their excellent performance and prospects.
COORDINATION CHEMISTRY REVIEWS
(2022)
Review
Chemistry, Multidisciplinary
Wei Li, Gabriele S. Kaminski Schierle, Bingfu Lei, Yingliang Liu, Clemens F. Kaminski
Summary: Super-resolution imaging techniques have become popular for visualizing cellular structures with nanometric resolution. This review focuses on the use of fluorescent nanoparticles (NPs) as bright and photostable probes for super-resolution imaging. The review provides an overview of different super-resolution methods, discusses their demands on the properties of fluorescent NPs, and reviews the features, strengths, and weaknesses of each NP class. The future outlook and opportunities in material science for the development of probes for multiplexed subcellular imaging with nanometric resolution are also discussed.
Article
Multidisciplinary Sciences
Agnieszka Pierzynska-Mach, Alberto Diaspro, Francesca Cella Zanacchi
Summary: This study used single-molecule localization microscopy (SMLM) to analyze the nanoscale organization of DEK protein. It found differences in cluster features of DEK protein in different breast cell types and showed that the nanoscale organization of DEK clusters remains stable regardless of protein overexpression or chromatin compaction changes.
Article
Optics
Brendan M. Heffernan, Peter S. Riley, Omkar D. Supekar, Stephanie A. Meyer, Diego Restrepo, Mark E. Siemens, Emily A. Gibson, Juliet T. Gopinath
Summary: This article presents a solution for imaging sub-diffraction dynamics of neural nanostructures involved in learning and memory using a two-photon, fiber-coupled, stimulated emission depletion microscope. The capabilities of this technique are demonstrated through super-resolution imaging of mammalian cells.
Article
Multidisciplinary Sciences
Joaquim Torra, Celine Lafaye, Luca Signor, Sylvain Aumonier, Cristina Flors, Xiaokun Shu, Santi Nonell, Guillaume Gotthard, Antoine Royant
SCIENTIFIC REPORTS
(2019)
Article
Chemistry, Multidisciplinary
Patricia Bondia, Joaquim Torra, Caterina M. Tone, Taka Sawazaki, Adrin del Valle, Begona Sot, Santi Nonell, Motomu Kanai, Youhei Sohma, Cristina Flors
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Nanoscience & Nanotechnology
Adrian del Valle, Joaquim Torra, Patricia Bondia, Caterina M. Tone, Patricia Pedraz, Virginia Vadillo-Rodriguez, Cristina Flors
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Applied
Roger Bresoli-Obach, Walter A. Massad, Abasi Abudulimu, Larry Lueer, Cristina Flors, Javier G. Luis, Laura I. Rosquete, Teresa A. Grillo, Ommid Anamimoghadam, Gotz Bucher, Santi Nonell
Summary: Researchers have developed aryl-substituted phenalenones inspired by the irreversible cyclization of 9-phenylphenalenone plant metabolites to yield highly-colored naphthoxanthenes. The lifetime of the naphthoxanthene photoisomer ranges from tens of picoseconds to tens of milliseconds depending on the electronic properties of the 9-aryl group.
Article
Chemistry, Multidisciplinary
Joaquim Torra, Felipe Viela, Diego Megias, Begona Sot, Cristina Flors
Summary: CRANAD-2, a fluorogenic curcumin derivative, shows good performance in both stimulated emission depletion (STED) and single-molecule localization microscopy (SMLM) for near-infrared detection and imaging of amyloid aggregates with a resolution range of 45-55 nm. Its versatility and performance make it a powerful tool for near-infrared nanoscopic imaging of amyloids in vitro and in vivo.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Medicinal
Ingrid Ortega, Joaquim Torra, Cristina Flors
Summary: This study investigates the effect of photodynamic treatment on the oscillations of MinD, a cell division regulator in Escherichia coli, using real-time single-cell fluorescence imaging. The results show that the concentration of methylene blue, a photosensitizer, disrupts the oscillation pattern of MinD. In contrast to antibiotics, photodynamic treatment abruptly interrupts the oscillation, reflecting different physiological mechanisms leading to bacterial death.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Joaquim Torra, Taka Sawazaki, Patricia Bondia, Santi Nonell, Motomu Kanai, Youhei Sohma, Cristina Flors
Summary: The study discusses the use of iodinated boron dipyrromethene photocatalysts as theranostic compounds against pathogenic bacterial biofilms. These compounds selectively stain and weaken the mechanical barrier of the biofilm, allowing conventional antimicrobial agents to regain their activity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Biochemistry & Molecular Biology
Ingrid V. Ortega, Felipe Viela, Cristina Flors
Summary: Min oscillations are an intriguing mechanism used by Escherichia coli to monitor the effect of sublethal environmental challenges on bacterial physiology. This review discusses the use of Min oscillations as a reporting tool to monitor the effect of extracellular cationic compounds and explores its potential to visualize stress responses and understand bacterial cell death. The physiological meaning of changes in Min oscillation period and the benefits and limitations of using oscillations as a reporter in fluorescence microscopy are also discussed.
Article
Nanoscience & Nanotechnology
Felipe Viela, Ingrid V. Ortega, Jaime J. Hernandez, Isabel Rodriguez, Sara Moreno-Da Silva, Alejandro Lopez-Moreno, Emilio M. Perez, Cristina Flors
Summary: Mechanobactericidal nanomaterials are promising antimicrobial strategies that rely on mechanical interactions with bacteria and overcome antibiotic resistance. However, the detailed mechanisms behind the killing effects are not well understood. This study investigates the real-time response of bacteria to weak mechanical interactions with nanostructured topographies and nanodarts, shedding light on the complex mechanisms of mechanically induced bacterial death.
Article
Chemistry, Multidisciplinary
Patricia Bondia, Cristina Flors, Joaquim Torra
Summary: Thioflavin T (ThT), commonly used as a fluorescent probe for amyloid detection, has been discovered to exhibit photodynamic activity against bacterial biofilms. It disrupts the complex architecture of biofilm matrix and efficiently inactivates bacterial cells, while also synergistically enhancing the activity of conventional antimicrobials.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Joaquim Torra, Patricia Bondia, Sylvia Gutierrez-Erlandsson, Begona Sot, Cristina Flors
Meeting Abstract
Biophysics
A. Del Valle, P. Bondia, C. M. Tone, V. Vadillo, C. Flors
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Meeting Abstract
Biophysics
P. Bondia, C. M. Tone, J. Torra, A. Del Valle, B. Sot, Y. Sohma, M. Kanai, C. Flors
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Meeting Abstract
Biophysics
J. Torra, P. Bondia, B. Sot, C. Flors
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2019)
Article
Chemistry, Multidisciplinary
Soranyel Gonzalez-Carrero, Lorena Bareno, Elke Debroye, Cristina Martin, Patricia Bondia, Cristina Flors, Raquel E. Galian, Johan Hofkens, Julia Perez-Prieto
CHEMICAL COMMUNICATIONS
(2019)
