Article
Chemistry, Multidisciplinary
Hanglong Wu, Teng Li, Sai P. Maddala, Zafeiris J. Khalil, Rick R. M. Joosten, Brahim Mezari, Emiel J. M. Hensen, Gijsbertus de With, Heiner Friedrich, Jeroen A. van Bokhoven, Joseph P. Patterson
Summary: A distributed electron microscopy method is proposed for studying complex chemical reactions, demonstrated through studying the desilication mechanism of zeolite crystals. The reaction is shown to proceed via a two-step anisotropic etching process, with defects in curved surfaces and between subunits controlling desilication kinetics.
Review
Chemistry, Multidisciplinary
Andrzej M. Zak
Summary: This article provides a summary and critical review of all in situ experiments using light, discusses various technical possibilities for introducing radiation into transmission electron microscopy, and highlights the most important differences between the effects of light and electrons on studied matter. Furthermore, it summarizes the most promising directions for further research using light excitation.
Article
Biochemistry & Molecular Biology
Mart G. F. Last, Lenard M. Voortman, Thomas H. Sharp
Summary: In cryo-transmission electron microscopy (cryo-TEM), sample thickness plays a crucial role in image quality. We propose a method using reflected light microscopy and machine learning to assess sample thickness prior to TEM imaging, accurately predicting the thickness of cryo-TEM samples. Our approach demonstrates high similarity between predicted and measured sample thickness, and the open-source software is freely available.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Article
Cell Biology
Jose M. Serra Lleti, Anna M. Steyer, Nicole L. Schieber, Beate Neumann, Christian Tischer, Volker Hilsenstein, Mike Holtstrom, David Unrau, Robert Kirmse, John M. Lucocq, Rainer Pepperkok, Yannick Schwab
Summary: This study presents a toolset for adherent cells that enables tracking and finding cells in FIB-SEM, along with automatic acquisition of high-resolution volume datasets. The workflow is completely automated using computer vision techniques, reducing or eliminating operator supervision.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Microbiology
Monika Kakol, Ezher Tagliasacchi, Andrzej Borkowski, Miroslaw Slowakiewicz
Summary: The nutritional condition of seed mussels used in New Zealand's Greenshell mussel aquaculture industry shows high variability, which may contribute to the marked variation observed in spat performance. Environmental factors that limit feeding during harvest and transport of spat are likely influencing the quality of spat used by the industry. Measures to improve the quality of wild spat supply, such as testing nutritional condition and developing nursery culture methods and artificial spat feeds, are needed.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Zewen Kang, Junyu Zhang, Xiaohua Guo, Yangfan Mao, Zhimin Yang, Ranjith Kumar Kankala, Peng Zhao, Ai-Zheng Chen
Summary: Metal oxides have attracted significant interest as catalysts in various reactions, and transmission electron microscopy (TEM) has been used to study their morphology, phase, structure, and chemical information. Recent advances in liquid-phase TEM have enabled the real-time imaging of metal oxides in solution, allowing for the characterization of their dynamic structure, morphology, and composition under working conditions. This review introduces the recent progress in liquid cells, specifically closed liquid cell chips, and discusses the advances in particle growth, phase transformation, self-assembly, core-shell nanostructure growth, and chemical etching. The application of liquid-phase TEM in understanding fundamental processes of metal oxides for CO2 reduction and water-splitting reactions is also highlighted, along with the future outlook and challenges in this research field.
Review
Chemistry, Physical
Shiwei Tao, Ming Li, Miaoqiang Lyu, Lingbing Ran, Roger Wepf, Ian Gentle, Ruth Knibbe
Summary: This paper reviews the opportunities and obstacles of closed-cell in operando TEM systems and compares them with open-cell configurations. By observing the processes in closed-cell configurations, a more reliable understanding of the electrochemical setups of most ion batteries under development can be obtained. However, the closed-cell configuration sacrifices imaging resolution.
Article
Biochemical Research Methods
Chidinma A. Okolo, Ilias Kounatidis, Johannes Groen, Kamal L. Nahas, Stefan Balint, Thomas M. Fish, Mohamed A. Koronfel, Aitziber L. Cortajarena, Ian M. Dobbie, Eva Pereiro, Maria Harkiolaki
Summary: This protocol describes sample preparation strategies for correlative 3D cryo-structured illumination microscopy and cryo-soft X-ray tomography, including a direct comparison and recommendations regarding fiducial selection. By combining different microscopy techniques, nanometer resolution imaging can be achieved under cryogenic conditions. Strategic use of markers is crucial for experimental design.
Article
Neurosciences
Karl Friedrichsen, Pratyush Ramakrishna, Jen-Chun Hsiang, Katia Valkova, Daniel Kerschensteiner, Josh L. Morgan
Summary: Correlated light and electron microscopy (CLEM) combines functional and molecular characterizations of neurons with anatomical maps of synaptic organization. This paper introduces a multiresolution approach to CLEM (mrCLEM), which efficiently targets electron microscopy (EM) imaging to optically characterized cells while maintaining optimal tissue preparation. By iteratively matching features visible in both low-resolution EM and densely labeled optical maps, high-resolution EM image acquisition and annotation can be targeted to cells of interest.
FRONTIERS IN NEURAL CIRCUITS
(2022)
Article
Microscopy
Misa Hayashida, Marek Malac, Jun Yamasaki
Summary: Bright-field transmission electron microscope (BFTEM) images exhibit spurious image intensity near the sample edge, which can be explained by considering the angular and energy loss distribution of the scattered electrons and lens aberrations. The spurious intensity can be significantly reduced by using electron energy filtering or a small collection semiangle.
Article
Energy & Fuels
Kaleb Lippiatt, Stuart Bell, Teng-Cheong Ong, Chris East, Donald McAuley, Geoffrey Will, Ted Steinberg
Summary: Proper materialographic sample preparation is crucial in electron microscopy to achieve high-quality material results, with the use of kerosene as a lubricant showing to be the best option for analyzing molten salt corrosion phenomena.
SOLAR ENERGY MATERIALS AND SOLAR CELLS
(2021)
Article
Microscopy
Sercan Keskin, Carly Pawell, Niels de Jonge
Summary: The article discusses methods for verifying the presence of water in graphene liquid cells (GLCs), emphasizing the need to critically consider liquid filling in GLC sample preparation due to the limitations of using a single criterion. The most reliable method is direct observation of the water exciton peak using electron energy loss spectroscopy (EELS), but alternative methods include analyzing image contrast, presence of bubbles, and an oxygen signal in the EEL spectrum. Nanoparticle movement in GLC is not always guaranteed.
Article
Microscopy
Abid Zulfiqar, Sana Azim, Eduardo Ortega, Niels de Jonge
Summary: This passage introduces the purpose of MATLAB scripts, which is to compute the sample-limited spatial resolution in TEM and STEM. The scripts can be used to select the best microscopy modality and optimize experimental conditions considering both electron optics and sample limitations. The spatial resolution is dependent on various parameters including material type, matrix type, depth, and electron dose.
Article
Materials Science, Composites
Yitong Ding, Qian Zhou, Xingwei Li, Ying Xiong, Shaoyun Guo
Summary: In this study, a melt blending method was used to prepare light-scattering materials with improved performance. The scattering range and uniformity were enhanced while the percolation threshold of scattering angle was reduced.
COMPOSITES SCIENCE AND TECHNOLOGY
(2021)
Review
Chemistry, Physical
Peter D. Dahlberg, W. E. Moerner
Summary: srCryoCLEM is an emerging method that combines the advantages of super-resolution fluorescence microscopy and cryogenic electron tomography, providing higher resolution and more informative data. The method is still in its infancy with many technical challenges to be overcome.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 72
(2021)
Article
Biochemistry & Molecular Biology
Diana B. Peckys, Daniel Gaa, Dalia Alansary, Barbara A. Niemeyer, Niels de Jonge
Summary: The study found that ORAI1 channels can form supra-molecular clusters, and different structures can be formed during SOCE activation, rather than simply being trapped near active STIM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Sana Azim, Lindsey A. Bultema, Michiel B. de Kock, Ernesto Rafael Osorio-Blanco, Marcelo Calderon, Josef Gonschior, Jan-Philipp Leimkohl, Friedjof Tellkamp, Robert Buecker, Eike C. Schulz, Sercan Keskin, Niels de Jonge, Gunther H. Kassier, R. J. Dwayne Miller
Summary: Liquid-phase transmission electron microscopy is a technique that allows for simultaneous imaging of sample structure and dynamics in a liquid environment. By adjusting the thickness of the liquid layer and controlling the electron dose, high-resolution observations of low mass-thickness contrast objects can be achieved. The use of an environmental liquid cell (ELC) enables researchers to study hydration effects and investigate liquid samples in situ.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Ecology
Sarah Aherfi, Djamal Brahim Belhaouari, Lucile Pinault, Jean-Pierre Baudoin, Philippe Decloquement, Jonatas Abrahao, Philippe Colson, Anthony Levasseur, David C. Lamb, Eric Chabriere, Didier Raoult, Bernard La Scola
Summary: The discovery and study of Acanthamoeba polyphaga Mimivirus and Pandoravirus massiliensis provided insights into the potential energy production mechanisms in giant viruses and the unique characteristics of their protein coding genome.
Article
Cell Biology
Sonia Brun, Eloine Bestion, Eric Raymond, Firas Bassissi, Zuzana Macek Jilkova, Soraya Mezouar, Madani Rachid, Marie Novello, Jennifer Tracz, Ahmed Hamai, Gilles Lalmanach, Lise Vanderlynden, Raphael Legouffe, Jonathan Stauber, Thomas Schubert, Maximilian G. Plach, Jerome Courcambeck, Cyrille Drouot, Guillaume Jacquemot, Cindy Serdjebi, Gael Roth, Jean-Pierre Baudoin, Christelle Ansaldi, Thomas Decaens, Philippe Halfon
Summary: The new autophagy inhibitor GNS561 shows high liver tropism and potent antitumor activity, particularly against hepatocellular carcinoma. Its mechanism of action involves lysosomal disruption and induction of cell death in tumor cells.
Article
Virology
Eloine Bestion, Keivan Zandi, Sandrine Belouzard, Julien Andreani, Hubert Lepidi, Marie Novello, Clara Rouquairol, Jean-Pierre Baudoin, Madani Rachid, Bernard La Scola, Jean-Louis Mege, Jean Dubuisson, Raymond F. Schinazi, Soraya Mezouar, Philippe Halfon
Summary: Since December 2019, the rapid spread of SARS-CoV-2 worldwide has caused over 280 million confirmed cases and 5 million deaths. Researchers have found that coronaviruses can hijack the autophagic process to facilitate viral replication. GNS561, a late-stage autophagy inhibitor, has shown antiviral activity against SARS-CoV-2 with a range of 6-40 nM, making it the most powerful molecule investigated for SARS-CoV-2 infection. GNS561 disrupts the autophagy mechanism and reduces SARS-CoV-2 virions in a mouse model.
Article
Chemistry, Multidisciplinary
Arixin Bo, Yawei Liu, Bjoern Kuttich, Tobias Kraus, Asaph Widmer-Cooper, Niels de Jonge
Summary: Self-assembly of nanoscale structures at liquid-solid interfaces plays a crucial role in industrial processes and natural phenomena. The conventional theory is oversimplified, and in situ observations using liquid-phase scanning transmission electron microscopy are required. It has been found that modifying the molecular coating of the substrate can control the self-assembly structures of nanoparticles.
ADVANCED MATERIALS
(2022)
Article
Immunology
Ikram Omar Osman, Clemence Garrec, Gabriel Augusto Pires de Souza, Ana Zarubica, Djamal Brahim Belhaouari, Jean-Pierre Baudoin, Hubert Lepidi, Jean-Louis Mege, Bernard Malissen, Bernard La Scola, Christian Albert Devaux
Summary: COVID-19 is the largest pandemic in this century, and it is not only characterized by respiratory damage, but also by gastrointestinal symptoms. This study suggests that SARS-CoV-2 may cause damage to the epithelial barrier of the intestines by disrupting E-cadherin adherens junctions, leading to gastrointestinal symptoms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jamir Priesner, Tobias Kraus, Niels de Jonge
Summary: This study presents a method that utilizes the electron beam of an environmental scanning electron microscope to manipulate gold nanoparticles (AuNPs) at the liquid-vapor interface. The controlled motion and agglomeration of AuNPs enable the creation of superstructures that float at the interface. The attractive force exerted by the electron beam on AuNPs is independent of zeta potential and has a larger spatial range than electrostatic interactions. The growth rate of agglomerates depends on the electron flux and electron beam energy. The hypothesis that local liquid evaporation caused by electron beam heating is responsible for the electron beam-induced AuNP assembly process is proposed.
ADVANCED MATERIALS INTERFACES
(2022)
Review
Immunology
Nadji Hannachi, Florent Arregle, Hubert Lepidi, Jean-Pierre Baudoin, Frederique Gouriet, Helene Martel, Sandrine Hubert, Benoit Desnues, Alberto Riberi, Jean-Paul Casalta, Gilbert Habib, Laurence Camoin-Jau
Summary: Whipple's disease is a chronic multisystemic infection caused by Tropheryma whipplei. It is often misunderstood due to its intracellular localization and absence of pathognomonic signs, leading to misdiagnosis. This case report highlights a patient initially treated for presumed rheumatoid arthritis who was later diagnosed with Whipple's disease after presenting with infectious endocarditis. The atypical presentation observed might be attributed to the anti-inflammatory treatment given for the initial misdiagnosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microscopy
Peter Kunnas, Mohammad-Amin Moradi, Nico Sommerdijk, Niels de Jonge
Summary: This paper examines a strategy for optimizing LP-EM experiments and develops a method for quantifying image quality at ultra low electron dose using STEM. By studying a test case, the authors show that precise knowledge of material density and the use of annular dark field STEM can improve experimental results.
Article
Virology
Marion Le Bideau, Lea Robresco, Jean-Pierre Baudoin, Bernard La Scola
Summary: There is a need for new rapid viral diagnostic electron microscopy methods, with scanning electron microscopy showing advantages in screening large specimens.
Article
Dentistry, Oral Surgery & Medicine
Djamal Brahim Belhaouari, Jean-Pierre Baudoin, Jean-Christophe Lagier, Virginie Monnet-Corti, Bernard La Scola, Angeline Antezack
Summary: The emerging coronavirus pneumonia epidemic caused by SARS-CoV-2 has spread rapidly worldwide. The main routes of transmission are recognized as aerosol/droplet inhalation. This study investigates the presence of SARS-CoV-2 particles in oral samples collected from symptomatic COVID-19 patients, using scanning electron microscopy and immunofluorescence staining. The results suggest that the oral cavity may be a potential niche for SARS-CoV-2 infection and transmission.
EUROPEAN JOURNAL OF ORAL SCIENCES
(2022)
Article
Microscopy
Abid Zulfiqar, Sana Azim, Eduardo Ortega, Niels de Jonge
Summary: This passage introduces the purpose of MATLAB scripts, which is to compute the sample-limited spatial resolution in TEM and STEM. The scripts can be used to select the best microscopy modality and optimize experimental conditions considering both electron optics and sample limitations. The spatial resolution is dependent on various parameters including material type, matrix type, depth, and electron dose.
Article
Cell Biology
Amina Ezzeroug Ezzraimi, Jean-Pierre Baudoin, Antoine Mariotti, Laurence Camoin-Jau
Summary: The study investigated the structure of platelet aggregates induced by different E. coli strains and the ultrastructure of platelet-E. coli mixtures using SEM and TEM. The results showed that the appearance of platelet aggregates was mainly dependent on the strain used, indicating a potential relationship between the structure of E. coli strains and platelet activation.
Article
Nanoscience & Nanotechnology
Arixin Bo, Tobias Kraus, Niels de Jonge
Summary: Self-assembled gold nanoparticles in superlattices can coalesce at temperatures below the bulk melting point, which could be applied in nanodevice manufacture.
ACS APPLIED NANO MATERIALS
(2023)
