期刊
JOURNAL OF MICROSCOPY
卷 240, 期 1, 页码 32-37出版社
WILEY
DOI: 10.1111/j.1365-2818.2010.03381.x
关键词
Cortical bone; cortical porosity; histology; micro-CT; rat; synchrotron
类别
资金
- Natural Science and Engineering Research Council of Canada [3536182008]
P>Although micro-computed tomography (micro-CT) has become the gold standard for assessing the 3D structure of trabecular bone, its extension to cortical bone microstructure has been relatively limited. Desktop micro-CT has been employed to assess cortical bone porosity of humans, whereas that of smaller animals, such as mice and rats, has thus far only been imaged using synchrotron-based micro-CT. The goal of this study was to determine if it is possible to visualize and quantify rat cortical porosity using desktop micro-CT. Tibiae (n = 10) from 30-week-old female Sprague-Dawley rats were imaged with micro-CT (3 mu m nominal resolution) and sequential ground sections were then prepared. Bland-Altman plots were constructed to compare per cent porosity and mean canal diameter from micro-CT (3D) versus histology (2D). The mean difference or bias (histology - micro-CT; +/- 95% confidence interval) for per cent porosity was found to be -0.15% (+/- 2.57%), which was not significantly different from zero (P = 0.720). Canal diameter had a bias (+/- 95% confidence interval) of -5.73 mu m (+/- 4.02 mu m) which was found to be significantly different from zero (P < 0.001). The results indicated that cortical porosity in rat bone can indeed be visualized by desktop micro-CT. Quantitative assessment of per cent porosity provided unbiased results, whereas direct analysis of mean canal diameter was overestimated by micro-CT. Thus, although higher resolution, such as that available from synchrotron micro-CT, may ultimately be required for precise geometric measurements, desktop micro-CT - which is far more accessible - is capable of yielding comparable measures of porosity and holds great promise for assessment of the 3D arrangement of cortical porosity in the rat.
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