期刊
JOURNAL OF MICROSCOPY
卷 233, 期 1, 页码 1-4出版社
WILEY
DOI: 10.1111/j.1365-2818.2008.03088.x
关键词
Computed tomography; critical point drying; en bloc staining; fern root; sample preparation; 3-D
类别
Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.
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