期刊
JOURNAL OF MICROSCOPY
卷 230, 期 3, 页码 353-362出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2008.01993.x
关键词
confocal laser scanning microscopy; data analysis; dimensionality of diffusion; distribution of diffusion coefficients; fluorescence recovery after photo-bleaching
类别
A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is verified utilizing FRAP experiments on mixtures of differently sized fluorescent micro-spheres as realizations of discrete D distributions.
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