期刊
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
卷 20, 期 4, 页码 797-799出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JMEMS.2011.2153826
关键词
Artificial lipid bilayer membrane; electrical detection; membrane microchamber; nanopore protein
类别
资金
- Japan Science and Technology Agency
- New Energy and Industrial Technology Development Organization, Japan
This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers that were bridged by gold patterns on a glass substrate. We succeeded in the formation of a lipid bilayer membrane at the mouth of the chamber and applied electrical potential to the membrane with the integrated component. We confirmed the reconstitution of a transmembrane nanopore protein into the formed lipid bilayer membrane by signal monitoring with the electrical connection.
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