期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 19, 期 6, 页码 582-587出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.0808.499
关键词
Acrylic acid; acrylonitrile; Arthrobacter nitroguajacolicus; nitrilase; calcium alginate; polyethylenimine
资金
- Major Basic Research Development Program of China [2007C13714306]
- Fund of the National High Technology Research
- Development Program of China [2006AA02Z241]
- Doctor Program of High Education of China [20051033701]
Immobilized cells of Arthrobacter nitroguajacolicus ZJUTB06-99 capable of producing nitrilase were used for biotransformation of acrylonitrile to acrylic acid. Six different entrapment matrixes were chosen to search for a suitable support in terms of nitrilase activity. Ca-alginate proved to be more advantageous over other counterparts in improvement of the biocatalyst activity and bead mechanical strength. The effects of sodium alginate concentration, CaCl2 concentration, bead diameter, and ratio by weight of cells to alginate, on biosynthesis of acrylic acid by immobilized cells were investigated. Maximum activity was obtained under the conditions of 1.5% sodium alginate concentration, 3.0%. CaCl2 concentration, and 2-mm bead size. The beads coated with 0.10% polyethylenimine (PEI) and 0.75% glutaraldehyde (GA) could tolerate more phosphate and decrease leakage amounts of cells from the gel. The beads treated with PEI/GA could be reused up to 20 batches without obvious decrease in activities, which increased about 100% compared with the untreated beads with a longevity of 11 batches.
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