Article
Microbiology
Xiaoyu Liu, Mingyang Zhou, Rui Sun, Shu Xing, Tao Wu, Hailun He, Jianbin Chen, John Kevin Bielicki
Summary: This study identified a novel esterase gene (Est33) from an Antarctic bacterium and characterized its biochemical properties. The Est33 enzyme was found to be a cold-active esterase with optimal activity towards a specific substrate.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xinchun Ran, Yaoyukun Jiang, Qianzhen Shao, Zhongyue J. Yang
Summary: EnzyKR is a new approach based on a deep learning framework for predicting enantiomeric outcomes in hydrolase-catalyzed kinetic resolution reactions. It automates the selection of enzyme scaffolds for separating a racemic substrate mixture and achieves good prediction performance using a structure-based encoding strategy.
Article
Biochemistry & Molecular Biology
Astrid Hoeppner, Alexander Bollinger, Stefanie Kobus, Stephan Thies, Cristina Coscolin, Manuel Ferrer, Karl-Erich Jaeger, Sander H. J. Smits
Summary: Bacterial lipolytic enzymes of family IV, homologs of mammalian hormone-sensitive lipases, exhibit broad substrate specificity and remarkable ability for various types of hydrolysis. Crystal structure analysis of a novel family IV esterase reveals insights into its substrate promiscuity and the important role of the cap domain in enzyme-substrate interaction. Structural data of PtEst1 are available in the worldwide protein data bank under accession codes 6Z68 and 6Z69.
Article
Environmental Sciences
Yan-Yan Liu, Yi-Xin Zhang, Hua-Mei Wen, Xiao-Long Liu, Xin-Jiong Fan
Summary: The cold-adapted esterase Est1260 from Mao-tofu metagenome was found to degrade PAEs and parabens at low temperatures. After site-saturation mutation, the variant A1B6 exhibited improved thermal stability without sacrificing activity at low temperatures. Est1260 and its variants were activated by NaCl and showed resistance to high concentrations of saline, making them potential biocatalysts for remediation of PAE and paraben-polluted environments.
Article
Biochemistry & Molecular Biology
Isabel Cea-Rama, Cristina Coscolin, Jose L. Gonzalez-Alfonso, Jog Raj, Marko Vasiljevic, Francisco J. Plou, Manuel Ferrer, Julia Sanz-Aparicio
Summary: The crystal structure of a novel family VIII esterase is reported, which provides insights into its broad substrate specificity. The unique structural elements of this esterase, including an open and exposed active site, flexible regions for substrate binding, and small residues enabling the entrance of large substrates, contribute to its broad substrate range.
Article
Chemistry, Analytical
Lei Feng, Ying Deng, Shufan Song, Yanqiu Sun, Jingnan Cui, Xiaochi Ma, Lingling Jin, Yan Wang, Tony D. James, Chao Wang
Summary: This study developed a fluorescence visualization tool to investigate the functional role of gut microbiota associated with obesity and disorders of lipid metabolism.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Andre Mong Jie Ng, Hongfang Zhang, Giang Kien Truc Nguyen
Summary: The study introduces a new method for detecting lipolytic enzyme activity which is 1000-fold more sensitive than previous methods, allowing for detection of as low as 5pg and 180pg of lipolytic activity. By adjusting buffering capacity, the detection sensitivity can be controlled, facilitating screening of both high and low abundance lipolytic enzymes. Coupled with liquid chromatography-mass spectrometry, this method provides a useful tool for sensitive detection and identification of lipolytic enzymes.
Article
Biotechnology & Applied Microbiology
Marco Distaso, Isabel Cea-Rama, Cristina Coscolin, Tatyana N. N. Chernikova, Hai Tran, Manuel Ferrer, Julia Sanz-Aparicio, Peter N. N. Golyshin
Summary: Metagenomics was used to build a metagenomic library and screen for a novel esterase from the Sorghum bicolor rhizosphere. The esterase EH0 showed a broad substrate specificity and a unique structural feature. This research provides new insights into the relationship between enzyme structure and function.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Dennis Sander, Yanfei Yu, Premankur Sukul, Sina Schaekermann, Julia E. Bandow, Trinetra Mukherjee, Subhra Kanti Mukhopadhyay, Lars Leichert
Summary: Lipolytic enzymes are important biocatalysts produced by various organisms and widely used in industrial applications. A new metaproteomics approach was used to identify a novel thermo- and solvent-stable lipolytic enzyme, DS-007, which exhibited high hydrolytic activity with substrates of shorter chain length.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Javier Rodriguez-Salarichs, Mario Garcia de Lacoba, Alicia Prieto, Maria Jesus Martinez, Jorge Barriuso
Summary: The study reveals that certain lipases from the Candida rugosa-like family exhibit wide substrate specificity on both triglycerides and sterol esters. By analyzing the full catalytic mechanisms of these enzymes, insights into their mechanistic properties have been gained. A computational protocol was developed to predict substrate specificity, using C. rugosa and O. piceae lipases as model enzymes and different triglycerides and cholesterol esters as model substrates, and the protocol was validated by comparing results with existing literature, offering a valuable tool for virtual screening of substrates for enzymes of the C. rugosa-like family with unknown catalytic properties.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Food Science & Technology
Xi He, Peng Liu, Xiaojian Tang, Ting Wang, Zhenshang Xu, Dongdong Hou, Dan Wu, Ning Han
Summary: In this study, the lp_1002 gene from Lactiplantibacillus plantarum WCFS1 was successfully cloned and overexpressed in L. plantarum WCFS1 strain. The recombinant strain exhibited higher lipase and esterase activities, and significantly reduced ester content during wine fermentation, indicating its potential application in food fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Shohreh Ariaeenejad, Kaveh Kavousi, Jian-Lin Han, Xue-Zhi Ding, Ghasem Hosseini Salekdeh
Summary: Enzymatic hydrolysis is a cheap and eco-friendly method for eliminating fatty substances from wastewater, and lipases can be utilized for bio-treatment of wastewater in various industrial applications. A novel stable lipase, PersiLipase1, was identified from tannery wastewater. It exhibited remarkable thermostability, maintained activity in a wide range of pH, and showed tolerance towards metal ions and organic solvents. The lipase demonstrated high efficiency in hydrolyzing olive oil and sheep fat, as well as in biotreatment of oil and grease from tannery wastewater.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Taresh P. Khobragade, Seongseon Yu, Hyunsang Jung, Mahesh D. Patil, Sharad Sarak, Amol D. Pagar, Hyunwoo Jeon, Somin Lee, Pritam Giri, Geon-Hee Kim, Sung-Su Cho, Seong-Hwa Park, Hye-Jung Park, Heung M. Kang, Sung R. Lee, Myeong S. Lee, Jeong H. Kim, In S. Choi, Hyungdon Yun
Summary: A bienzymatic cascade was developed for the synthesis of beta-amino acids as an intermediate for sitagliptin production. Recombinant Escherichia coli were used for whole-cell biotransformation to achieve high conversion rates. The desired product was obtained with excellent conversions in small-scale reactions, and sitagliptin phosphate was synthesized with high yield and purity.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Gautam Kumar Meghwanshi, Swati Verma, Vaibhav Srivastava, Rajender Kumar
Summary: This article discusses the importance of lipolytic enzymes in various industries and focuses on the current knowledge and future research directions of underexploited lipolytic enzymes sourced from the archaeal domain, as well as the structure and homology of archaeal lipases and esterases.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Yusuke Nakamichi, Tomohiko Matsuzawa, Masahiro Watanabe, Katsuro Yaoi
Summary: MeXyl31, a member of GH31 family, is an alpha-xylosidase that degrades xylosyl substrates. Its crystal structure reveals its tetrameric state and substrate recognition mechanism.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Nam Hee Kim, Ji-Hye Park, Eunsook Chung, Hyun-Ah So, Myung Hwan Lee, Jin-Cheol Kim, Eul Chul Hwang, Seon-Woo Lee
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
Article
Agriculture, Multidisciplinary
Aryan Rahimi-Midani, Kyoung-Ho Kim, Seon-Woo Lee, Sang Bong Jung, Tae-Jin Choi
Plant Pathology Journal
(2016)
Article
Agriculture, Multidisciplinary
Jungwook Park, Pyeong An Lee, Hyun-Hee Lee, Kihyuck Choi, Seon-Woo Lee, Young-Su Seo
PLANT PATHOLOGY JOURNAL
(2017)
Article
Microbiology
Raees Khan, Amir Zeb, Nazish Roy, Roniya Thapa Magar, Hyo Jeong Kim, Keun Woo Lee, Seon-Woo Lee
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Biotechnology & Applied Microbiology
Myung Hwan Lee, Raees Khan, Weixin Tao, Kihyuck Choi, Seung Yeup Lee, Jae Wook Lee, Eul Chul Hwang, Seon-Woo Lee
JOURNAL OF BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Raees Khan, Nazish Roy, Kihyuck Choi, Seon-Woo Lee
Article
Biotechnology & Applied Microbiology
Min-Jung Kwak, Hyun Gi Kong, Kihyuck Choi, Soon-Kyeong Kwon, Ju Yeon Song, Jidam Lee, Pyeong An Lee, Soo Yeon Choi, Minseok Seo, Hyoung Ju Lee, Eun Joo Jung, Hyein Park, Nazish Roy, Heebal Kim, Myeong Min Lee, Edward M. Rubin, Seon-Woo Lee, Jihyun F. Kim
NATURE BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Sang-Hoon Kim, Pyeoung-Ann Kang, Keetae Han, Seon-Woo Lee, Sangkee Rhee
Article
Microbiology
Eun Joo Jung, Soo Yeon Choi, Sang-Moo Lee, Ju Yeon Song, Hyoung Ju Lee, Eunjin Kim, Pyeong An Lee, Kihyuck Choi, Jihyun F. Kim, Seon-Woo Lee
Summary: A novel bacterial strain, Flavobacterium dauae sp. nov., was isolated from the rhizosphere of tomato plant grown in South Korea. It showed distinctive physiological characteristics and genetic relationship within the Flavobacterium genus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Roniya Thapa Magar, Seung Yeup Lee, Hyo Jeong Kim, Seon-Woo Lee
Summary: Bacteriophages have the potential to be promising alternative pesticides against bacterial diseases in crops. This study found that RpT1 and RpY2 phages effectively controlled bacterial wilt in tomato plants. The density, timing, and use of adjuvants of phages can influence their biocontrol efficacy.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Myoungjoo Riu, Choong-Min Ryu, Soohyun Lee, Seon-Woo Lee, Seungdon Lee, Sang-Keun Oh, Haeyoung Jeong
Summary: In this study, we sequenced the genome of Ralstonia pseudosolanacearum strain SL1931, isolated from pepper stems. This strain belongs to the phylotype I of R. solanacearum, the causal pathogen of bacterial wilt. We found that strain SL1931 has a distinct type III effector profile compared to the reference genome strain GMI1000.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Agriculture, Multidisciplinary
Kwang Yeol Baek, Hyun-Hee Lee, Geun Ju Son, Pyeong An Lee, Nazish Roy, Young-Su Seo, Seon-Woo Lee
PLANT PATHOLOGY JOURNAL
(2018)
Article
Biotechnology & Applied Microbiology
Shabir Ahmad, Seung Yeup Lee, Raees Khan, Hyun Gi Kong, Geun Ju Son, Nazish Roy, Kihyuck Choi, Seon-Woo Lee
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Riaz Ullah, Muhammad Yasir, Imran Khan, Fehmida Bibi, Sayed Sartaj Sohrab, Ahmed Al-Ansari, Fahad Al-Abbasi, Abdulmohsin A. Al-Sofyani, Ihsanullah Daur, Seon-Woo Lee, Esam I. Azhar
CANADIAN JOURNAL OF MICROBIOLOGY
(2017)
Article
Agriculture, Multidisciplinary
Heejung Cho, Eun-Sung Song, Young Kee Lee, Seungdon Lee, Seon-Woo Lee, Ara Jo, Byoung-Moo Lee, Jeong-Gu Kim, Ingyu Hwang
PLANT PATHOLOGY JOURNAL
(2018)