Article
Biochemistry & Molecular Biology
Jiahui Ren, Hong Duan, Haoxin Dong, Shuya Wu, Yongkun Du, Gaiping Zhang, Angke Zhang
Summary: The article "Treatment of Porcine Reproductive and Respiratory Syndrome Virus" introduces a protein called TAT-Nb1 that inhibits PRRSV infection and replication by delivering specific antibodies. The experimental results demonstrate that TAT-Nb1 can effectively suppress PRRSV replication and may serve as a new anti-PRRSV candidate drug.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Liwei Li, Sina Qiao, Guoxin Li, Wu Tong, Shishan Dong, Jiachen Liu, Ziqiang Guo, Haihong Zheng, Ran Zhao, Guangzhi Tong, Yanjun Zhou, Fei Gao
Summary: Since 2018, the African swine fever virus (ASFV) outbreak in China has caused significant economic losses. The emergence of less virulent ASFVs in 2020 has posed challenges for the early diagnosis and control of African swine fever (ASF) in China. This study successfully expressed ASFV p17 in CHO cells and established an indirect enzyme-linked immunosorbent assay (ELISA) based on purified p17. The monoclonal antibody against p17 recognized a conservative linear epitope and showed specific reactivity, making it suitable for the identification of recombinant porcine reproductive and respiratory syndrome virus (PRRSV) expressing p17.
Article
Medicine, General & Internal
Arwa A. Faizo, Thamir A. Alandijany, Ayman T. Abbas, Sayed S. Sohrab, Sherif A. El-Kafrawy, Ahmed M. Tolah, Ahmed M. Hassan, Esam I. Azhar
Summary: The developed COVID-19 NP IgG ELISA assay showed excellent sensitivity, specificity, and agreement, with high overall accuracy. It specifically detects IgG antibodies to the SARS-CoV-2 NP protein, without cross-detecting antibodies related to other coronaviruses.
Article
Microbiology
Li Yang, Qiang Xu, Bin Yang, Jiayu Li, Rong Dong, Jingjing Da, Zhixu Ye, Yongjie Xu, Hourong Zhou, Xiangyan Zhang, Lin Liu, Yan Zha, Fuxun Yu
Summary: This study established a highly sensitive ELISA system for the detection of SARS-CoV-2 IgG antibodies and found that higher antibody titers were correlated with more severe cases of COVID-19.
Article
Agriculture, Dairy & Animal Science
Szilvia Jakab, Krisztina Bali, Csongor Freytag, Anna Pataki, Eniko Feher, Mate Halas, Akos Jerzsele, Istvan Szabo, Krisztina Szarka, Adam Balint, Krisztian Banyai
Summary: Porcine reproductive and respiratory syndrome (PRRS) is a viral disease that causes significant economic losses globally. This study developed a targeted next-generation sequencing method for laboratory diagnosis and epidemiologic monitoring of the disease. The method demonstrated high sensitivity and effectiveness in detecting PRRSV in clinical serum samples, including mixed infections and virus microevolution. The findings suggest that amplicon deep sequencing of a conserved region of the PRRSV genome could be valuable for disease control and surveillance.
Article
Virology
Liling Zhou, Chuncong Mo, Yujie Yang, Zhichao Zhou, Aiping You, Ye Fan, Wenkuan Liu, Xiao Li, Rong Zhou, Xingui Tian
Summary: The ongoing COVID-19 pandemic has had a significant impact on the world, emphasizing the importance of accurate virus detection. In this study, researchers generated 90 monoclonal antibodies that exclusively reacted with the nucleocapsid protein of SARS-CoV-2. They identified major epitopes using enzyme-linked immunoassay and found that some antibodies showed no cross-reaction with other coronaviruses. These antibodies were used for antigen detection, and novel B-cell epitopes of the viral nucleocapsid protein were identified.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Essam H. Ibrahim, Hamed A. Ghramh, Mona Kilany
Summary: After the outbreak of the COVID-19 pandemic, the development of specific and sensitive enzyme immunoassay (EIA) has shown high specificity and sensitivity for detecting SARS-CoV-2, serving as a valuable tool for diagnostics.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Virology
Wenlong Wang, Yongning Zhang, Hanchun Yang
Summary: A blocking ELISA was developed for the diagnosis of porcine deltacoronavirus (PDCoV), with high diagnostic sensitivity and specificity, which can effectively control this pathogen that poses a threat to the swine industry and human health.
Article
Microbiology
Susanna K. P. Lau, Zirong He, Chi-Ching Tsang, Tony T. Y. Chan, Hayes K. H. Luk, Elaine Chan, Kenneth S. M. Li, Joshua Fung, Franklin W. N. Chow, Anthony R. Tam, Tom W. H. Chung, Sally C. Y. Wong, Tak-Lun Que, Kitty S. C. Fung, David C. Lung, Alan K. L. Wu, Ivan F. N. Hung, Jade L. L. Teng, Ulrich Wernery, Suk-Wai Hui, Paolo Martelli, Patrick C. Y. Woo
Summary: Researchers have developed a SARS-CoV-2 antibody detection method that can be used in a wide range of animals for COVID-19 surveillance.
Article
Veterinary Sciences
Hua Deng, Ning Xin, Fancong Zeng, Feng Wen, Heyou Yi, Chunquan Ma, Shujian Huang, Guihong Zhang, Yao Chen
Summary: This study identified two mutated amino acid sites, serine 78 and serine 99, in the nucleocapsid protein that can affect its function and significantly decrease the replication ability of PRRSV-2.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Marcelo S. Conzentino, Karl Forchhammer, Emanuel M. Souza, Fabio O. Pedrosa, Meri B. Nogueira, Sonia M. Raboni, Fabiane G. M. Rego, Dalila L. Zanette, Mateus N. Aoki, Jeanine M. Nardin, Bruna Fornazari, Hugo M. P. Morales, Paola A. F. Celedon, Carla V. P. Lima, Sibelle B. Mattar, Vanessa H. Lin, Luis G. Morello, Fabricio K. Marchini, Rodrigo A. Reis, Luciano F. Huergo
Summary: Serological assays play a crucial role in identifying previous exposure to SARS-CoV-2 and tracking COVID-19 cases. The study developed an ELISA method using purified SARS-CoV-2 nucleocapsid protein, showing high sensitivity and specificity, stable antigen-coated plates, and potential for mass production.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chia-Ming Su, Leyi Wang, Dongwan Yoo
Summary: SARS-CoV-2 proteins activate NF-kappa B, leading to the production of proinflammatory cytokines, which may contribute to acute respiratory distress syndrome in COVID-19 patients. Among these proteins, ORF7a plays a significant role in inducing inflammatory cytokines and chemokines expression.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Qi Ji, Guanggang Qu, Bing Liu, Yang Bai, Guihua Wang, Rui Chen, Xu Zheng, Zhigang Zhang, Yonglin Yang, Chunyan Wu
Summary: This study developed a double-antibody sandwich ELISA for pGM-CSF and investigated its effects during PRRSV infection. The results showed that PRRSV infection had minimal impact on pGM-CSF levels both in vitro and in vivo. Treatment with pGM-CSF did not inhibit PRRSV replication, and knockdown of pGM-CSF did not enhance replication either. Therapeutic antibody treatment increased serum pGM-CSF levels, which correlated with lower pneumonia incidence and intracellular PRRSV-RNA levels. These findings suggest a protective role played by pGM-CSF during PRRSV infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Ida Jarlhelt, Laura Perez-Alos, Rafael Bayarri-Olmos, Cecilie Bo Hansen, Maria Skaalum Petersen, Pal Weihe, Jose Juan Almagro Armenteros, Johannes Roth Madsen, Jacob Pohl Stangerup Nielsen, Linda Maria Hilsted, Kasper Karmark Iversen, Henning Bundgaard, Susanne Dam Nielsen, Peter Garred
Summary: The prediction of durability of immunity against COVID-19 is crucial, and longitudinal studies are necessary for understanding the details of protective SARS-CoV-2 antibody responses. The study developed an ELISA test based on the N protein to distinguish between infection- and vaccine-induced immune responses.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Maria Victoria Humbert, Precious Chinonyerem Opurum, Nathan J. Brendish, Stephen Poole, Peijun He, Ioannis Katis, Jerry Quaye, Yaw Bediako, Patrick Jacques Duriez, Robert W. Eason, Collin Sones, Osbourne Quaye, Gordon A. Awandare, Myron Christodoulides, Tristan W. Clark, Peter K. Quashie, Christopher J. McCormick
Summary: Controlling the spread of SARS-CoV-2 is challenging due to asymptomatic transmission. Low-cost ELISA assays show promise in community screening with good sensitivity and specificity, warranting further investigation.
JOURNAL OF INFECTION
(2022)