期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 90, 期 3, 页码 156-159出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2012.04.021
关键词
Batrachochytrium dendrobatidis; Chytrid; Magnetic capture hybridization; Whole genome amplification
资金
- National Science Foundation [DBI-0905810, DEB-0815315, DEB-1120249]
- Direct For Biological Sciences
- Division Of Environmental Biology [1120249] Funding Source: National Science Foundation
- Division Of Environmental Biology
- Direct For Biological Sciences [0815315] Funding Source: National Science Foundation
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0905810] Funding Source: National Science Foundation
We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream KR-based genomic applications. (C) 2012 Elsevier B.V. All rights reserved.
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