期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 85, 期 2, 页码 143-148出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2011.02.009
关键词
Bacillus anthracis; Spores; Exosporium; Extraction; Anthrax
资金
- Midwest Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research
- NIH [AI074589]
Biochemical studies of the outermost spore layers of the Bacillus cereus family are hindered by difficulties in efficient dispersal of the external spore layers and difficulties in dissociating protein complexes that comprise the exosporium layer. Detergent and physical methods have been utilized to disrupt the exosporium layer. Herein we compare commonly used SDS extraction buffers used to extract spore proteins and demonstrate the incomplete extractability of the exosporium layer by these methods. Sonication and bead beating methods for exosporium layer removal were also examined. A combination of genetic and physical methods is the most effective for isolating proteins found in the spore exosporium. (C) 2011 Elsevier B.V. All rights reserved.
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