期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 86, 期 2, 页码 243-247出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2011.05.004
关键词
Multiplex PCR; Plasmid profiling; Borrelia burgdorferi; Lyme disease
Borrelia burgdorferi has an unusual genomic structure containing 21 plasmids. These plasmids carry genes that are essential for infectivity and survival of the spirochetes in vivo. Several plasmids are lost during cultivation in vitro, which might lead to a heterogeneous population after multiple passages and loss of infectivity in laboratory animals. Herein, we present a simple and inexpensive multiplex PCR method that detects the complete plasmid profile of B. burgdorferi B31 in just two PCR tubes. (C) 2011 Elsevier B.V. All rights reserved.
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