期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 84, 期 1, 页码 140-143出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.11.005
关键词
E. coli; Cellular viability; CFU; Aerobic respiration; MTT reduction; Succinate dehydrogenase
资金
- Ministry of Science and Technology of China [2009ZX09306-008]
Four methods, namely enumeration of colony-forming units (CFU), aerobic respiration, MTT reduction capacity and succinate dehydrogenase activity were compared to determine the viability of E. coli cells at the early and late exponential growth phases. Our results revealed that great differences in cell viability existed between these methods and that the choice of method to determine cell viability must be made with caution. (C) 2010 Elsevier B.V. All rights reserved.
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