Article
Biochemistry & Molecular Biology
Chong Pang, Jianshuang Chen, Shuangyan Liu, Yingying Cao, Hao Miao
Summary: Shikonin exhibits broad-spectrum antifungal activity, particularly against fluconazole-resistant strains. It also demonstrates potent antifungal activities against other pathogenic fungi without apparent toxicity to mammalian cells. The mechanism of action involves the induction of apoptosis characteristics to combat Candida albicans infection.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Mohsen Rashidi, Ali Bazi, Alireza Ahmadzadeh, Orazio Romeo, Ali Rezaei-Matehkolaei, Mahdi Abastabar, Iman Haghani, Sepideh Mirzaei
Summary: The present study investigated the growth inhibitory and apoptotic effects of umbelliprenin against Candida albicans in a BALB/c mice model of disseminated candidiasis. Umbelliprenin was able to eradicate fungal infection in BALB/c mice and promote antifungal effects, partly via inducing apoptosis and inhibiting the expression of enzymes and genes involved in antifungal resistance and adhesins encoding genes.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Cell Biology
Yayun Chen, Qian Jiang, Furong Qing, Junxia Xue, Qiuxiang Xiao, Wenji He, Lina Sui, Zhiping Liu
Summary: MDA5 enhances host susceptibility to invasive C. albicans infection by inducing cell apoptosis and compromising macrophage functions.
Article
Biochemistry & Molecular Biology
Heesu Kim, Dong Gun Lee
Summary: Naringin-induced mitochondrial damage and dysfunction lead to oxidative stress and apoptosis in Candida albicans.
Article
Fisheries
Kai Liu, Zhaoxiang Sun, Chun Yang, Li Jan Lo, Jun Chen
Summary: The XPC protein is involved in recognizing DNA damage and initiating nucleotide excision repair. A zebrafish xpc knockout mutant was generated and found to have increased sensitivity to UV irradiation, leading to higher levels of DNA damage and apoptosis. In addition to its role in DNA repair, XPC also plays a role in other cellular processes.
Article
Biochemistry & Molecular Biology
Shuangyan Yao, Yuting Feng, Yan Zhang, Jinrong Feng
Summary: Cells face constant genetic assaults that cause DNA damage, leading to genome instability. Eukaryotic cells have evolved a DNA damage response system to monitor and repair these lesions. Studies show that DDR genes in pathogenic fungus Candida albicans are functionally similar to those in Saccharomyces cerevisiae, but may act through distinct mechanisms, particularly in resisting DNA damage stress induced by reactive oxygen species.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Microbiology
Chaerim Kim, Jae-Goo Kim, Ki-Young Kim
Summary: Although Candida albicans commonly colonizes on mucosal surfaces, it can cause candidiasis as an opportunistic infectious fungus. The emergence of resistant Candida strains and the toxicity of anti-fungal agents have led to the development of new potential anti-fungal agents. Sclareol, a labdane-type diterpene, has shown anti-Candida activity with a minimum inhibitory concentration of 50 mu g/mL in 24 h. It induces apoptosis-like cell death, suppresses biofilm and hyphal formation in C. albicans, and is considered as a novel anti-fungal agent and anti-virulence factor.
Article
Biology
Yuanyuan Wang, Yinhe Mao, Xiaoqing Chen, Xinhuang Huang, Zhongyi Jiang, Kaiyan Yang, Lixing Tian, Tong Jiang, Yun Zou, Xiaoyuan Ma, Chaoyue Xu, Zili Zhou, Xianwei Wu, Lei Pan, Huaping Liang, Lin Zhong, Changbin Chen, Antonis Rokas
Summary: The transition metal iron is important in living cells, but high levels can be toxic. A new study finds that a mutant lacking the iron-responsive transcription factor Hap43 is better able to colonize the gastrointestinal tract. The researchers demonstrate that high iron triggers post-translational modifications and degradation of Hap43, leading to reduced levels and increased expression of antioxidant genes. This study provides insight into the interplay between iron homeostasis and fungal colonization in the gut.
Article
Chemistry, Multidisciplinary
Robin Jeong, Hitendra Kumar, Steven Jones, Allen Sandwell, Keekyoung Kim, Simon S. Park
Summary: This study explores the improved sanitization potency of vaporous hydrogen peroxide (VHP) treatment on resilient bacterial spores while maintaining polymeric filter performance. By utilizing a batch fumigation chamber with hydrogen peroxide vapor and ozone, followed by intense pulsed light treatments, the study found that synergistic effects could achieve sterilization at reduced H2O2 concentrations under ambient conditions.
Article
Biotechnology & Applied Microbiology
Payal Gupta, Hrishikesh Gupta, Krishna Mohan Poluri
Summary: The research evaluated the antifungal activity of geraniol against Candida glabrata biofilm, discovering multiple mechanisms including biofilm eradication, cellular pathway interference, and induction of apoptosis. These findings strengthen the candidacy of geraniol as a novel antifungal therapeutic.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Kang Xiong, Chang Su, Qiangqiang Sun, Yang Lu
Summary: CAS5 and EFG1 are key transcription factors in the transcriptional response to cell wall stress induced by Echinocandins, coregulating the expression of caspofungin-responsive genes while also independently controlling induction of some genes. Additionally, they bind to caspofungin-responsive gene promoters to coordinately activate their expression.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Public, Environmental & Occupational Health
B. Knobling, G. Franke, C. Belmar Campos, H. Buettner, M. Christner, E. M. Klupp, P. M. Maurer, J. K. Knobloch
Summary: This study analyzed the susceptibility of clinical isolates of vancomycin-resistant enterococci (VRE) to UV-C radiation. The results showed that UV-C doses reported in the literature may be effective for commonly used reference strains, but may be insufficient for clinical isolates of VRE. Therefore, future studies should use the most tolerant clinical isolates to validate automated UV-C devices or longer exposure times should be expected to ensure efficacy in real-world settings.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Biochemistry & Molecular Biology
Mohmmad Younus Wani, Aijaz Ahmad, Faisal Mohammed Aqlan, Abdullah Saad Al-Bogami
Summary: This study evaluated the impact of a citral derivative on cell death in Candida albicans, resulting in apoptotic-type cell death.
BIOORGANIC CHEMISTRY
(2021)
Article
Immunology
Quan-Zhen Lv, De-Dong Li, Hua Han, Yi-Heng Yang, Jie-Lin Duan, Hui-Hui Ma, Yao Yu, Jiang-Ye Chen, Yuan-Ying Jiang, Xin-Ming Jia
Summary: Preconditioning with the FLO8-deficient mutant of C. albicans provides protection against subsequent lethal infections by inducing Dectin-2/CARD9-mediated IL-10 production. This protection is achieved by preventing C. albicans-induced apoptosis of thymic T cells, leading to enhanced Th1-biased immune responses against fungal infections.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Pierre Lapaquette, Amandine Ducreux, Louise Basmaciyan, Tracy Paradis, Fabienne Bon, Amandine Bataille, Pascale Winckler, Bernhard Hube, Christophe d'Enfert, Audrey Esclatine, Elisabeth Dubus, Marie-Agnes Bringer, Etienne Morel, Frederic Dalle
Summary: This study investigated the role of autophagy-related proteins in the infection of epithelial cells by Candida albicans. The results showed that autophagy-related proteins were recruited to invasion sites and autophagosomes were formed during active penetration of C. albicans into host cells. These autophagic events were associated with plasma membrane damage. Additionally, the autophagy-related proteins ATG5 and ATG16L1 played a crucial role in maintaining plasma membrane repair and protecting epithelial cells against C. albicans-induced cell death.
Letter
Critical Care Medicine
Vincenzo Russotto, Giacomo Bellani, John G. Laffey
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Mary Garvey, Neil J. Rowan
Summary: Fungal pathogens pose a significant threat to human health, especially among vulnerable individuals. The emergence of antifungal drug resistance has further complicated the treatment of cutaneous, invasive, and bloodstream infections. To tackle this crisis, the World Health Organization has prioritized fungal pathogens, emphasizing the urgent need for solutions. This review discusses the challenges of antifungal drug resistance, highlights the effectiveness of current disease mitigation approaches, and calls for a better understanding of fungal resistance mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Industrial
Evismar Andrade, Leo R. Quinlan, Richard Harte, Bronwyn Reid-McDermott, Frank Kirrane, Enda Fallon, Martina Kelly, Tony Hall, Michael Scully, John Laffey, Patrick Pladys, Ethel Ryan, Dara Byrne, Gearoid OLaighin
Summary: This study developed an online training platform to train clinicians on the use of a critical care medical device and carried out a preliminary evaluation of the platform via a case study. The results showed that participants were able to use and interpret the device interface and were overall satisfied with the training platform, perceiving it as an efficient and effective method for training.
APPLIED ERGONOMICS
(2023)
Article
Anesthesiology
Vincenzo Russotto, Jean Baptiste Lascarrou, Elena Tassistro, Matteo Parotto, Laura Antolini, Philippe Bauer, Konstanty Szuldrzynski, Luigi Camporota, Christian Putensen, Paolo Pelosi, Massimiliano Sorbello, Andy Higgs, Robert Greif, Giacomo Grasselli, Maria G. Valsecchi, Roberto Fumagalli, Giuseppe Foti, Pietro Caironi, Giacomo Bellani, John G. Laffey, NTUBE Study Investigators
Summary: Videolaryngoscopy is associated with higher first-pass intubation success rates in critically ill patients and does not increase the risk of major adverse events.
BRITISH JOURNAL OF ANAESTHESIA
(2023)
Review
Environmental Sciences
Neil J. Rowan
Summary: The COVID-19 pandemic was declared a global public health emergency by the WHO. There was a lack of knowledge on how to respond to the pandemic and manage the disease. Methods for safe reuse of personal protective equipment have been developed, and these methods can also be applied to decontaminate large volumes of waste. Effective disease mitigation strategies are needed to address the challenges posed by COVID-19 variants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Cheila Almeida, Jara Laso, David Baptista de Sousa, Ronan Cooney, Paula Quinteiro, Neil Rowan, Ana Claudia Dias, Eoghan Clifford, Rodrigo G. Reboredo, Maria Margallo, Maria Leonor Nunes, Antonio Marques
Summary: Packaging is crucial for food protection, consumer information, and waste reduction, but it can also negatively impact the environment. Recycling waste treatment offers more environmental benefits, yet only 66% of packaging waste is recycled in the EU. To minimize environmental impact, reducing packaging production and promoting reuse are the key approaches. Assessing consumer willingness to reduce environmental impact of seafood packaging is important.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Letter
Critical Care Medicine
Tai Pham, Leo Heunks, Giacomo Bellani, Laurent Brochard, John Laffey
LANCET RESPIRATORY MEDICINE
(2023)
Article
Critical Care Medicine
Bruno Francois, Simon Lambden, Tom Fivez, Sebastien Gibot, Marc Derive, Jean-Marie Grouin, Margarita Salcedo-Magguilli, Jeremie Lemarie, Nicolas De Schryver, Ville Jalkanen, Tarik Hicheur, Jean-Jacques Garaud, Valerie Cuvier, Ricard Ferrer, Morten Bestle, Ville Pettila, Jean-Paul Mira, Camille Bouisse, Emmanuelle Mercier, Joris Vermassen, Vincent Huberlant, Isabelle Vinatier, Nadia Anguel, Mitchell Levy, Pierre-Francois Laterre
Summary: This study aimed to investigate whether the TREM-1 inhibitor nangibotide could improve outcomes in patients with septic shock. The findings suggest that both low and high doses of nangibotide did not significantly improve the condition of patients with septic shock compared to placebo.
LANCET RESPIRATORY MEDICINE
(2023)
Article
Respiratory System
Bairbre A. McNicholas, Miguel Ibarra-Estrada, Yonatan Perez, Jie Li, Ivan Pavlov, Aileen Kharat, David L. Vines, Oriol Roca, David Cosgrave, Claude Guerin, Stephan Ehrmann, John G. Laffey
Summary: Awake prone positioning (APP) gained attention during the COVID-19 pandemic for treating acute hypoxaemic respiratory failure. It improves oxygenation and has similar effects as invasive ventilation in patients with moderate-severe acute respiratory distress syndrome. Randomised controlled studies in COVID-19 patients have conflicting outcomes, but evidence suggests that more severe hypoxaemic patients benefit most from APP when managed in higher care environments and being prone for several hours.
EUROPEAN RESPIRATORY REVIEW
(2023)
Review
Immunology
Elaine Meade, Neil Rowan, Mary Garvey
Summary: Emerging, re-emerging, and zoonotic viral pathogens pose a serious threat to human health and global stability. In order to prevent future outbreaks, manufacturing and distribution methods need to be changed to advance the production of vaccines, monoclonal antibodies, cytokines, and other antiviral therapies.
Article
Medicine, General & Internal
Hector E. Gonzalez, Sean D. McCarthy, Claire Masterson, John G. Laffey, Ronan MacLoughlin, Daniel O'Toole
Summary: Mesenchymal stem cell secretome has strong immunomodulatory effects and can be delivered to the lung through nebulization, showing therapeutic potential for inflammatory diseases such as pneumonia ARDS.
FRONTIERS IN MEDICINE
(2023)
Article
Anesthesiology
David M. Hannon, Tim Jones, Jack Conolly, Conor Judge, Talha Iqbal, Atif Shahzad, Michael Madden, Frank Kirrane, Peter Conneely, Brian H. Harte, Martin O'Halloran, John G. Laffey
Summary: Developed and assessed a system for shared ventilation using clinically available components to individualize tidal volumes. The system allowed accurate and reproducible titration of tidal volumes to each limb over a range of ventilator settings and simulated lung conditions.
BMC ANESTHESIOLOGY
(2023)
Article
Critical Care Medicine
Marius Troseid, Jose R. Arribas, Lambert Assoumou, Aleksander Rygh Holten, Julien Poissy, Vida Terzic, Fulvia Mazzaferri, Jesus Rodriguez Bano, Joe Eustace, Maya Hites, Michael Joannidis, Jose-Artur Paiva, Jean Reuter, Isabel Puentmann, Thale D. J. H. Patrick-Brown, Elin Westerheim, Katerina Nezvalova-Henriksen, Lydie Beniguel, Tuva Borresdatter Dahl, Maude Bouscambert, Monika Halanova, Zoltan Peterfi, Sotirios Tsiodras, Michael Rezek, Matthias Briel, Serhat Unal, Martin Schlegel, Florence Ader, Karine Lacombe, Cecilie Delphin Amdal, Serge Rodrigues, Kristian Tonby, Alexandre Gaudet, Lars Heggelund, Joy Mootien, Asgeir Johannessen, Jannicke Horjen Moller, Beatriz Diaz Pollan, Anders Aune Tveita, Anders Benjamin Kildal, Jean-Christophe Richard, Olav Dalgard, Victoria Charlotte Simensen, Aliou Balde, Lucie de Gastines, Marta del Alamo, Burc Aydin, Fridtjof Lund-Johansen, Mary-Anne Trabaud, Alpha Diallo, Bente Halvorsen, John-Arne Rottingen, Evelina Tacconelli, Yazdan Yazdanpanah, Inge C. Olsen, Dominique Costagliola
Summary: The study conducted a placebo-controlled trial of baricitinib in severe/critical COVID cases. The results showed that baricitinib did not reduce mortality within 60 days, and there may be safety risks in vaccinated participants, which require further investigation.
Meeting Abstract
Critical Care Medicine
Jie Li, Jian Luo, Ivan Pavlov, Yonatan Perez, Aileen Kharat, Elsa Tavernier, David Vines, Oriol Roca, Bairbre McNicholas, Miguel Ibarra-Estrada, John Laffey, Claude Guerin, Stephan Ehrmann
CRITICAL CARE MEDICINE
(2023)
Article
Critical Care Medicine
Bairbre A. A. McNicholas, Emanuele Rezoagli, Andrew J. J. Simpkin, Sankalp Khanna, Jacky Y. Y. Suen, Pauline Yeung, Daniel Brodie, Gianluigi Li Bassi, Tai Pham, Giacomo Bellani, John F. F. Fraser, John Laffey
Summary: AKI is a common and serious complication in COVID-19 patients, with different characteristics and outcomes compared to non-COVID-19 ARDS patients.