期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 87, 期 1, 页码 125-127出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2011.07.014
关键词
Intestinal mucosa; DNA extraction; Bead-beating; Quantitative PCR
资金
- Carle Foundation
- Carle Hospital-University of Illinois
Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies. (C) 2011 Elsevier B.V. All rights reserved.
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