期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 79, 期 3, 页码 353-357出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2009.10.007
关键词
Cre/loxP; Marker-less mutations
资金
- National Science Foundation [MCB-0543187]
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE016686] Funding Source: NIH RePORTER
Although targeted mutagenesis of Streptococcus pneumoniae is readily accomplished with the aid of natural genetic transformation and chimeric donor DNA constructs assembled in vitro, the drug resistance markers often employed for selection of recombinant products can themselves be undesirable by-products of the genetic manipulation. A new cassette carrying the erythromycin-resistance marker ermAM that can be used as a temporary marker for selection of desired recombinants is described. The cassette may subsequently be removed at will by virtue of an embedded fucose-regulated Cre recombinase gene and terminal lox66 and lox71 Cre recognition sites, with retention of 34 bp from the cassette as an inert residual double-mutant lox72 site. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据