A MALDI TOF MS-based minisequencing method for rapid detection of TEM-type extended-spectrum beta-lactamases in clinical strains of Enterobacteriaceae

A minisequencing method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was developed for rapid identification of single nucleotide polymorphisms at bla(TEM) gene codons 104, 164 and 238 associated with extended-spectrum activity on TEM-type betalactamases. The method was validated by testing the Escherichia coli and Klebsiella pneumoniae strains possessing the known blaTEM gene sequences. (C) 2008 Elsevier B.V. All rights reserved.