Article
Biochemistry & Molecular Biology
Daniel Leon-Aparicio, Jorge Arreola, Patricia Perez-Cornejo, Alfredo Sanchez-Solano
Summary: The study shows that oleic acid irreversibly blocks the TMEM16A chloride channel in a dose- and voltage-dependent manner, possibly through an allosteric mechanism. This blockage is intensified by increasing the extracellular anion concentration and is independent of voltage for certain pore mutants.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Article
Biochemistry & Molecular Biology
Motoki Okui, Tatsuro Murakami, Hongxin Sun, Chiaki Ikeshita, Narisato Kanamura, Akiyuki Taruno
Summary: CALHM1 and 3 subunits are N-glycosylated at single Asn residues in their second extracellular loops, controlling the biosynthesis and gating kinetics of the CALHM1/3 channel. CALHM3 is reversibly palmitoylated at three intracellular Cys residues, with palmitoylation playing a critical role in CALHM1/3 activity.
Article
Biochemistry & Molecular Biology
Daniel Barth, Andreas Lueckhoff, Frank J. P. Kuehn
Summary: Recent studies have found that PIP2 plays a crucial role in activating TRPM2 channels, with human TRPM2 being the least sensitive to PIP2 among different species variants. This suggests that changes in intramembranous PIP2 content can regulate the activity of TRPM2 channels in a species-specific manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Z. Chen, D. H. Terman, S. P. Travers, J. B. Travers
Summary: Responses in the gustatory nucleus of the solitary tract (rNST) are modulated by synaptic interactions and membrane properties. The potassium current I-A is a potential modulator. This study explored the role of I-A in modulating the response to afferent stimulation and GABA-mediated inhibition in the rNST using transgenic mice expressing channelrhodopsin in GABAergic neurons.
Article
Biology
Crescent L. Combe, Carol M. Upchurch, Carmen C. Canavier, Sonia Gasparini
Summary: A combination of in vitro electrophysiology and modeling identified TRPM4 channels as major drivers of cholinergic modulation of firing rate in rat CA1 pyramidal neurons. Cholinergic agonist carbachol reverses the spike rate adaptation caused by Na-V channel inactivation, resulting in more spikes on the down-ramp compared to the up-ramp. Pharmacological experiments and modeling suggest that TRPM4 channels play a role in the cholinergic shift of firing center of mass through Ca2+-activated nonspecific cation current ICAN.
Article
Pharmacology & Pharmacy
Lin Yan, Haixia Ren, Fangting Yuan, Wenyao Shi, Ying Wang, Hesheng Luo
Summary: Apelin-13 inhibits the spontaneous contractile activity of isolated rat colons by suppressing L-type calcium channels and BKCa channels in colonic smooth muscle cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Milica Gusic, Klaus Benndorf, Christian Sattler
Summary: Our study reveals that each occupied binding site contributes to channel activation in P2X7 receptors. While the occupation of a single binding site can activate the channels, three bound ligands maximally stabilize the open state, suggesting a stepwise activation process for P2X7 receptors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Biochemistry & Molecular Biology
Tengiz Oniani, Laura Vinnenberg, Rahul Chaudhary, Julian A. Schreiber, Kathrin Riske, Brandon Williams, Hans-Christian Pape, John A. White, Anna Junker, Guiscard Seebohm, Sven G. Meuth, Petra Hundehege, Thomas Budde, Mehrnoush Zobeiri
Summary: This study investigates the effects of MS-related factors on HCN channels and finds that HCN channels are not directly influenced by trace metals and divalent cation chelators.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Annalisa Bernareggi, Martina Zangari, Andrew Constanti, Paola Zacchi, Violetta Borelli, Alessandro Mangogna, Paola Lorenzon, Giuliano Zabucchi
Summary: This study investigates the effect of crocidolite asbestos fibers on chloride conductance in Xenopus oocyte cell membranes. The results show that exposure to asbestos enhances chloride currents in the membranes, and this enhancement is influenced by calcium levels and specific blockers of TMEM16A Ca2+-activated chloride channels.
Article
Pharmacology & Pharmacy
Ioannis Markakis, Ioannis Charitakis, Christine Beeton, Melpomeni Galani, Elpida Repousi, Stella Aggeloglou, Petros P. Sfikakis, Michael W. Pennington, K. George Chandy, Cornelia Poulopoulou
Summary: The study investigated the expression of Kv1.3 channels in T lymphocytes of patients with multiple sclerosis, demonstrating a significant up-regulation compared to control groups. Interestingly, there was a significant difference in Kv1.3 channel density between secondary progressive and relapsing-remitting multiple sclerosis patients. These findings suggest that Kv1.3 channels could serve as potential therapeutic targets and peripheral biomarkers for multiple sclerosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Engineering, Chemical
Ming Wang, Mengxin Li, Zhaohui Fei, Jiakun Li, Zhongyi Ren, Yingfei Hou
Summary: In this study, a novel polyamine called Cyclen is used as the monomer to construct a nanofiltration membrane. Through a surfactant-assembly regulated interfacial polymerization (SARIP) process, the phase transfer of Cyclen is intensified, resulting in a membrane with high permeance and perm-selectivity.
Article
Neurosciences
Kevin Jehasse, Laurent Massotte, Sebastian Hartmann, Romain Vitello, Sofian Ringlet, Marie Vitello, Han Chow Chua, Stephan A. Pless, Dominique Engel, Jean-Francois Liegeois, Bernard Lakaye, Jochen Roeper, Vincent Seutin
Summary: The research showed that the compound XG selectively inhibits the pacemaking of dopamine neurons without affecting their bursting behavior. It demonstrated specificity in its effects on different types of neurons and seemed to target a small subthreshold XG-sensitive current carried by Na+ and Cl- ions. The molecular target of XG and its precise mechanism of action remain to be elucidated, but these findings represent a significant step towards understanding pacemaking in dopamine neurons.
Article
Nanoscience & Nanotechnology
Jiaru Fang, Xinwei Wei, Hongbo Li, Ning Hu, Xingxing Liu, Dongxin Xu, Tao Zhang, Hao Wan, Ping Wang, Xi Xie
Summary: Cardiovascular diseases pose a significant threat to human health, but drug development is time-consuming and costly. Researchers have developed a drug assessment platform based on an electrical-mechanical synchronized biosensing system, which allows for high-throughput and time-dependent assessment. The system can record and analyze dynamic responses of cardiomyocytes to drugs, enabling rapid and effective drug screening.
MICROSYSTEMS & NANOENGINEERING
(2021)
Review
Pharmacology & Pharmacy
Damian C. Bell, Luigi Leanza, Saverio Gentile, Daniel R. Sauter
Summary: Ion channels play key roles in various physiological processes, and their dysfunction can lead to channelopathies, including cancer. The field of ion channel and cancer research has rapidly grown in the past two decades, allowing us to gain deeper insights into the mechanisms of ion channels in cancer and develop therapeutic interventions.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Plant Sciences
Li Wang, Hongtao Tu, Hui Hou, Zengqiang Zhou, Hongbo Yuan, Chaoxi Luo, Qinsheng Gu
Summary: Botryosphaeria dothidea was found to exhibit resistance to carbendazim, a devastating disease affecting apple crops. 19 isolates were found to have stable carbendazim resistance and a specific mutation in the beta-tubulin gene. A LAMP method was developed for rapid identification of this mutation, providing a valuable tool for monitoring and controlling resistance in apple crops.
Article
Engineering, Biomedical
Kaizheng Liu, Maury Wiendels, Hongbo Yuan, Changshun Ruan, Paul H. J. Kouwer
Summary: This article emphasizes the importance of three-dimensional hydrogel models in understanding and predicting cell behavior, highlighting the reciprocal interactions between cells and the extracellular matrix in regulating tissue functions. The structure, uses, advantages, and limitations of commonly used biopolymer networks, such as collagen, fibrin gels, and polyisocyanide gel, are discussed to provide guidance for tissue engineers and biophysicists in designing and utilizing these materials for 3D cell culture purposes.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Boying Li, Shuxi Ren, Dong Gao, Ning Li, Manman Wu, Hongbo Yuan, Mei Zhou, Chengfen Xing
Summary: TRPA1 ion channels are overexpressed in breast cancer cells and enhance cellular resistance to oxidative stress through upregulating the anti-apoptotic pathway. Near-infrared light-responsive conjugated polymer nanoparticles are designed to regulate TRPA1 ion channels and promote apoptosis of breast cancer cells, reducing drug resistance and suppressing tumor growth.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Microbiology
Hongbo Yuan, Bingke Shi, Li Wang, Tianxiang Huang, Zengqiang Zhou, Hui Hou, Hongtao Tu
Summary: In this study, Bacillus velezensis strain P2-1 isolated from apple branches showed strong inhibitory effects against Botryosphaeria dothidea, a major postharvest pathogen of apples. The strain inhibited the growth of B. dothidea, secreted antifungal substances, and induced apple defense responses. These findings suggest that B. velezensis strain P2-1 could be used as a biocontrol agent against B. dothidea-induced apple postharvest decay.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Hongbo Yuan, Jiajun Zhu, Qifan Wang, Man Cheng, Zhenjiang Cai
Summary: This paper presents an improved DeepLab v3+ network for the segmentation of grapevine leaf black rot spots and demonstrates its superior performance on test sets.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Polymer Science
Zhanshuo Cao, Hongbo Yuan, Ning Li, Xiaoning Liu, Xiongwei Qu, Chengfen Xing
Summary: This article introduces a biomimetic PIC/HA composite material constructed through enzymatic biomineralization strategy. The composite material has excellent mechanical properties and higher sensitivity to mechanical stress, showing great potential in bone tissue engineering.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Energy & Fuels
Yanhong Luo, Hongbo Yuan, Xinying Wang, Ye Li, Jiangping Jing, Tao Da
Summary: This paper investigates the problem of energy planning in energy network and proposes an optimal scheduling strategy and a distributed cooperative control method. The proposed approach considers thermal inertia and renewable energy in the energy scheduling model, leading to economical and environmentally friendly operations in micro-energy networks. A real-time control using Linear Quadratic Gaussian (LQG) is used to ensure stable operation, and a dynamic stratification strategy is designed to optimize resource usage.
Review
Agriculture, Multidisciplinary
Zhenjiang Cai, Jialiang Cui, Hongbo Yuan, Man Cheng
Summary: Animal body temperature is closely related to its metabolism and life activities, reflecting the physiological and health status of animals. Infrared thermography (IRT) as a non-invasive measurement technology has been gradually applied in animal temperature measurement, allowing in-depth analysis of animal health and understanding of physiological changes. This paper summarized the research achievements of IRT in animal temperature detection and discussed its application cases, problems, and future development.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Polymer Science
Zhiyuan Si, Xinhai Zhao, Xinbo Ren, Chao Zheng, Hongbo Yuan, Ji Liu, Libin Song
Summary: In order to address the low transverse tensile strength issue of triaxial geogrid, a performance-optimized multi-axial geogrid (POMG) capable of withstanding larger transverse loads was designed. The forming equipment and process of POMG were designed, and through formability and mechanical property tests, POMG with good formability and mechanical properties, especially the one with circular and semicircular holes, achieved the highest average tensile strength exceeding 16 KN/m. Numerical simulation further validated the feasibility of the process and provided guidance on shape distribution and stress-strain behavior of POMG during the forming process.
Article
Microbiology
Hongbo Yuan, Mengjia Yuan, Bingke Shi, Zhuoni Wang, Tianxiang Huang, Jiahong Zhu, Hui Hou, Li Wang, Hongtao Tu
Summary: Apple ring rot, caused by Botryosphaeria dothidea, is a common postharvest disease of apple. This study investigated the biocontrol activity and mechanism of Bacillus halotolerans strain Pl7 against B. dothidea. Results showed that strain Pl7 exhibited strong inhibitory activity against B. dothidea and its culture filtrate possessed antifungal activity. Further analysis revealed that the culture filtrate of strain Pl7 could increase cell membrane permeabilization of B. dothidea. In apple fruit, strain Pl7 suppressed ring rot and showed the ability to colonize and thrive in fruit wounds. Transcriptome studies indicated that strain Pl7 mainly affected genes involved in plant secondary metabolite biosynthesis and plant-pathogen interaction. Overall, B. halotolerans strain Pl7 shows promise as a biocontrol agent against apple postharvest decay.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Yadong Liu, Hongbo Yuan, Xin Zhao, Caihu Fan, Man Cheng
Summary: The study presents a reconstruction method based on dual RGB-D cameras for quickly and accurately establishing a 3D model of the peanut plant. The method is 2.54 times faster than the widely used ICP algorithm with approximate accuracy. It offers a potential tool for improving traits and agronomic qualities of plants.
Article
Multidisciplinary Sciences
Hongbo Yuan, Kaizheng Liu, Mar Condor, Jorge Barrasa-Fano, Boris Louis, Johannes Vandaele, Paula de Almeida, Quinten Coucke, Wen Chen, Egbert Oosterwijk, Chengfen Xing, Hans Van Oosterwyck, Paul H. J. Kouwer, Susana Rocha
Summary: Cells sense external forces from the extracellular matrix (ECM) and generate contractile forces to stiffen and remodel the matrix. However, the underlying mechanisms of this bidirectional mechanical exchange are still poorly understood. This study uses a synthetic hydrogel based on polyisocyanide (PIC) polymers to investigate cell-matrix interactions and demonstrates the potential of PIC gels in studying mechanobiology and designing materials.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Anesthesiology
Xianghong Lian, Yunzhu Lin, Ting Luo, Yang Jing, Hongbo Yuan, Yixin Guo
Summary: This study aimed to assess the efficacy and safety of esketamine as an adjunct to propofol for sedation during endoscopic procedures in patients. The results showed that the administration of esketamine reduced the recovery time compared to normal saline and required a lower propofol dosage. However, caution should be exercised due to the possibility of visual disturbance associated with esketamine use.
BMC ANESTHESIOLOGY
(2023)
Review
Medicine, General & Internal
Grammati Sarri, Elisabetta Patorno, Hongbo Yuan, Jianfei (Jeff) Guo, Dimitri Bennett, Xuerong Wen, Andrew R. Zullo, Joan Largent, Mary Panaccio, Mugdha Gokhale, Daniela Claudia Moga, M. Sanni Ali, Thomas P. A. Debray
Summary: This study developed a guidance using a mixed methods approach to direct researchers and healthcare decision-makers on when and how to combine evidence from non-randomised studies (NRS) and randomised controlled trials (RCTs) to enhance transparency and confidence. The framework consists of seven steps and offers recommendations on statistical approaches based on different healthcare decision-making scenarios.
BMJ EVIDENCE-BASED MEDICINE
(2022)
Article
Plant Sciences
Hongbo Yuan, Hui Hou, Zengqiang Zhou, Hongtao Tu, Li Wang
Summary: In this study, a simple LAMP approach was developed to rapidly detect the E198A mutation in apple bitter rot-causing C. gloeosporioides isolates. The optimized LAMP protocol allowed for the genotype determination of a given isolate within 60 minutes of incubation at 63 degrees C using four specific primers. The results of the reaction could be visually interpreted based on a fluorescent yellow-green color change and were further confirmed via gel electrophoresis. Importantly, this LAMP assay was capable of rapidly and reliably detecting apples infected with carbendazim-resistant isolates harboring the E198A mutation.
HORTICULTURAL PLANT JOURNAL
(2022)
