Article
Chemistry, Applied
Michael A. Pitino, Sharon Unger, Alain Doyen, Yves Pouliot, Akash Kothari, Melanie Sergius-Ronot, Richard P. Bazinet, Debbie Stone, Deborah L. O'Connor
Summary: Holder pasteurization (HoP) can lead to denaturation and aggregation of bioactive proteins, while high pressure processing (HPP) minimally affects bioactive components. In vitro digestion showed that HoP increased proteolysis of lactoferrin and milk fat globule membrane proteins. Lysozyme activity was significantly impacted by HoP digestion compared to HPP or raw milk. HPP-treated donor milk is digested similarly to raw milk, while preserving functional bioactive proteins.
Article
Food Science & Technology
Mariana Sisconeto Bisinotto, Daniele Cristina da Silva, Luciana de Carvalho Fino, Fernando Moreira Simabuco, Rosangela Maria Neves Bezerra, Adriane Elisabete Costa Antunes, Maria Teresa Bertoldo Pacheco
Summary: Cashew nuts, mainly consumed as snacks, have gained interest in broken kernels for their added value. The study evaluated the antioxidant capacity and potential prebiotic effect of defatted cashew nut flour through simulated gastrointestinal digestion, showing significant increase in antioxidant capacity in the soluble digested fraction and potential prebiotic effect in the insoluble digested fraction. The high protein content and presence of antioxidant peptides make defatted cashew nut flour a promising functional food ingredient.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Applied
Marzia Giribaldi, Stefano Nebbia, Valerie Briard-Bion, Julien Jardin, Chiara Peila, Alessandra Coscia, Didier Dupont, Laura Cavallarin, Amelie Deglaire
Summary: The study found that High Temperature-Short Time pasteurization and Holder pasteurization have different effects on peptide release in human milk during simulated preterm infant gastrointestinal digestion. The peptidomic profile of human milk differed according to the pasteurization type, with HTST pasteurization showing a greater similarity to raw milk.
Article
Food Science & Technology
Juliana A. S. Leite, Randall C. Robinson, Jaime Salcedo, Juliana N. R. Ract, Virginia S. Quintal, Carmen C. Tadini, Daniela Barile
Summary: This study investigated the impact of microwave-assisted heating on the concentration of key biological compounds in donor human milk. Compared to low-temperature long-time pasteurization, microwave-assisted heating better preserved the concentration of immunoglobulins and lactoferrin, making it a promising alternative method for Human Milk Banks.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Alexis Ayala-Nino, Araceli Castaneda-Ovando, Judith Jaimez-Ordaz, Gabriela Mariana Rodriguez-Serrano, Jose Antonio Sanchez-Franco, Luis Guillermo Gonzalez-Olivares
Summary: This study demonstrated that in vitro enzymatic digestion allows the release of multifunctional bioactive peptide fractions from amaranth proteins.
NATURAL PRODUCT RESEARCH
(2022)
Article
Food Science & Technology
Simona Rinaldi, Sabrina Di Giovanni, Giuliano Palocci, Michela Conto, Roberto Steri, Carmela Tripaldi
Summary: This study investigated the impact of milk heat pre-treatment and pasteurization level on the protein digestibility of produced cheese. In vitro digestion method was used to analyze the peptide profile and amino acids released in digestion. The results showed the importance of heat treatment management on protein digestibility in soft cheese.
Article
Food Science & Technology
Melanie Sergius-Ronot, Shyam Suwal, Michael A. Pitino, Sara Shama, Sharon Unger, Deborah L. O'Connor, Yves Pouliot, Alain Doyen
Summary: The impact of different processing methods on the digestibility of human milk protein concentrate (HMPC) was evaluated. Results showed that heat treatments and high hydrostatic pressure (HHP) affected the release of certain amino acids during digestion. However, overall digestibility was similar between pasteurized and raw HMPC. The non-thermal HHP process was found to preserve the bioactive proteins in HMPC during digestion.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Ton Baars, Betty van Esch, Luuk van Ooijen, Zuomin Zhang, Pieter Dekker, Sjef Boeren, Mara Diks, Johan Garssen, Kasper Hettinga, Remco Kort
Summary: This study aimed to characterize the microbiota and peptidomic composition of raw milk kefir and investigate its potential anti-allergic effects using validated research models for food allergy. The raw milk kefir was produced with a defined freeze-dried starter culture, and samples were taken during fermentation at seven time intervals. Peptide compositions were analyzed in the raw and heat-treated milk, as well as the kefir products made from these milks. The results showed that only raw milk kefir suppressed the acute allergic skin response in sensitized mice, indicating its potential health benefits.
Article
Food Science & Technology
Jiayu Zhang, Mengdi Li, Ying Lv, Shuntang Guo, Baichong Yang
Summary: The study investigated the protein aggregates and in vitro digestibility of soymilk, as well as dry-heated soybeans (DHS) at 160 degrees C and 200 degrees C. The results showed that thermal treatment led to the formation of different types of insoluble protein aggregates. The in vitro digestibility was higher for 160 degrees C DHS compared to soymilk and 200 degrees C DHS.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Miguel A. Alvarez-Olguin, Lilia M. Beltran-Barrientos, Adrian Hernandez-Mendoza, Aaron F. Gonzalez-Cordova, Belinda Vallejo-Cordoba
Summary: The bioaccessibility and bioavailability of food-derived bioactive compounds are crucial in assessing their physiological effects. Various in vitro models, such as the static simulated gastrointestinal digestion model and the Caco-2 cell monolayer model, have been developed for evaluating the stability and absorption of these compounds. Further exploration of ex vivo tissue-based models like the everted sac may offer a more physiological and cost-effective approach for studying bioactive peptides.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Chemistry, Applied
Dorota Martysiak-Zurowska, Edyta Malinowska-Panczyk, Malgorzata Orzolek, Bogumila Kielbratowska, Elena Sinkiewicz-Darol
Summary: Bioactive substances in human milk are important for premature newborns. Microwave heating compared to convection heating preserves more active proteins in human milk, but may degrade certain components like lysozyme. Controlled conditions of microwave heating are better for preserving the bioactive components of human milk.
Article
Chemistry, Applied
Yavuz Beceren, Murat Gurbuz, Jale Catak, Busra Demir, Halime Ugur, Mustafa Yaman
Summary: This study examined the bioaccessibility of vitamin C in baby biscuits and follow-on milk. The results showed that the measured vitamin C concentrations were generally lower than the declared amounts on the labels. In comparison, naturally occurring vitamin C in UHT cow's milk had higher bioaccessibility than the added vitamin C in baby biscuits.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Applied
Eslim Sugey Sandoval-Sicairos, Ada Keila Milan-Noris, Diego Armando Luna-Vital, Jorge Milan-Carrillo, Alvaro Montoya-Rodriguez
Summary: This study identified bioactive peptides, especially anti-inflammatory ones, released from germinated amaranth during in vitro gastrointestinal digestion, showing potential for combating non-communicable diseases.
Article
Food Science & Technology
Teagan S. Edwards, Krista L. Dawson, Jacqueline I. Keenan, Andrew S. Day
Summary: The study utilized a simple in vitro digestion method to investigate the release of biologically active peptides from casein isolated from regular and A2 milk. Results showed that regular milk casein released significantly more BCM-7 than A2 milk casein during digestion, providing a platform for further research on the gut health benefits of A2 compared to A1 milk.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Chemistry, Applied
Simonetta Caira, Gabriella Pinto, Gianluca Picariello, Paola Vitaglione, Sabrina De Pascale, Andrea Scaloni, Francesco Addeo
Summary: A purification procedure followed by mass spectrometry analysis was used to identify milk-derived peptides in the plasma of healthy volunteers after consuming pasteurized milk. The study revealed individual variability in the presence of peptides from various bovine milk proteins, including caseins and beta-lactoglobulin. The potential bioactivity and immunogenicity of these peptides and their effects on human health require further investigation.
Review
Nutrition & Dietetics
Yasuaki Wada, Yasuhiro Takeda, Masashi Kuwahata
Article
Nutrition & Dietetics
Masashi Kuwahata, Yukiko Kobayashi, Yasuaki Wada, Wataru Aoi, Yasuhiro Kido
NUTRITION RESEARCH
(2018)
Article
Chemistry, Applied
Yosuke Komatsu, Yasuaki Wada, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda, Tohru Hira, Hiroshi Hara
Article
Nutrition & Dietetics
Yasuaki Wada, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda
JOURNAL OF NUTRITION
(2020)
Review
Endocrinology & Metabolism
Yasuaki Wada, Bo Lonnerdal
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE
(2020)
Article
Nutrition & Dietetics
Yosuke Komatsu, Yasuaki Wada, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda, Tomoyasu Aizawa
Summary: The study found that urinary levels of taurine and TMAO respond significantly to protein undernutrition, with corresponding changes in hepatic levels of these metabolites and gene expressions associated with their metabolic pathways. Taurine and TMAO could therefore serve as non-invasive urinary biomarker candidates for detecting protein undernutrition.
BRITISH JOURNAL OF NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Fuka Tabata, Yasuaki Wada, Satomi Kawakami, Kazuhiro Miyaji
Summary: Serum albumin, the most abundant circulating protein in mammals, has three isoforms based on its redox state, traditionally considered a biomarker of systemic oxidative stress but recent research indicates oxidized albumin itself may worsen pathological conditions. Experimental evidence also suggests its potential as a sensitive protein nutrition biomarker.
Article
Nutrition & Dietetics
Yasuaki Wada, Tatsuya Ehara, Fuka Tabata, Yosuke Komatsu, Hirohisa Izumi, Satomi Kawakami, Kiwamu Noshiro, Takeshi Umazume, Yasuhiro Takeda
Summary: In a study of Japanese pregnant women, it was found that maternal serum reduced ALB ratio in the third trimester is positively correlated with infant birth weight. A rat model also showed a positive correlation between maternal serum ALB redox state and birth weight, indicating the importance of maternal protein nutritional status in infant birth weight.
Article
Agriculture, Multidisciplinary
Yosuke Komatsu, Muneya Tsuda, Yasuaki Wada, Takuya Shibasaki, Hirohiko Nakamura, Kazuhiro Miyaji
Summary: This study assessed the protein quality of milk-, plant-, and insect-based protein materials using the INFOGEST method. Milk-based protein materials had the highest protein digestibility (86.1-90.8%), followed by soy (85.1%) and wheat (82.3%). These materials had significantly higher protein digestibility compared with zein (65.1%), cricket (63.6%), and mealworm (69.5%). Additionally, milk-based protein materials had higher protein quality than plant- and insect-based protein materials based on the INFOGEST digestion method.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Yosuke Komatsu, Yasuaki Wada, Fuka Tabata, Satomi Kawakami, Yasuhiro Takeda, Kiminori Nakamura, Tokiyoshi Ayabe, Koshi Nakamura, Takashi Kimura, Akiko Tamakoshi
Summary: This study aimed to investigate the influence of maternal diet on macronutrient content in human milk and the extent to which this variation affects infant growth in the first month of life. The study found that maternal retinol intake was associated with the carbohydrate content in human milk, and the energy content in human milk was associated with an increase in weight standard deviation score at 1 month of age.
Article
Nutrition & Dietetics
Fuka Tabata, Yasuaki Wada, Takuya Shibasaki, Satomi Kawakami, Mayumi Inubashiri, Masayoshi Hosaka, Kiwamu Noshiro, Takeshi Umazume, Kazuhiro Miyaji
Summary: An observational study of 115 Japanese pregnant women found that the serum reduced albumin ratio was positively correlated with gestation length during the third trimester and may contribute to healthier gestation outcomes. Protein intake was significantly and positively correlated with the serum reduced albumin ratio in the second trimester.
NUTRITION RESEARCH
(2023)
Article
Food Science & Technology
Mai Morozumi, Yasuaki Wada, Muneya Tsuda, Fuka Tabata, Tatsuya Ehara, Hirohiko Nakamura, Kazuhiro Miyaji
Summary: This study investigated the bifidogenic effects of cow's glycomacropeptide (GMP) and metabolic enzymes that Bifidobacterium species use. The results showed that Bifidobacterium bifidum JCM1254 can grow in a medium containing GMP, and the growth is dependent on the presence of glycans. Carbohydrate metabolism-related proteins, including sialidase, were more abundant when Bifidobacterium bifidum JCM1254 was cultured in the GMP-containing medium. Additionally, GMP glycans can be utilized by other bifidobacteria, and the cross-feeding process involves carbohydrate metabolism-related proteins expressed by Bifidobacterium bifidum.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Nutrition & Dietetics
Fuka Tabata, Yasuaki Wada, Satomi Kawakami, Akiko Tamakoshi, Kazuhiro Miyaji
Summary: This study aimed to develop a method for estimating the ratio of HMA to total albumin (%HMA) without using expensive analyzers. The method involved removing HMA, determining total albumin and HNA concentrations, and calculating %HMA. Proof-of-concept and validation experiments confirmed the correlation between the estimated %HMA values and the values obtained by HPLC.
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
(2023)
Article
Nutrition & Dietetics
Yasuaki Wada, Xijier, Namiko Seto, Yosuke Komatsu, Muneya Tsuda, Yohei Kitamura, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda
FRONTIERS IN NUTRITION
(2019)
Article
Nutrition & Dietetics
Yasuaki Wada, Yosuke Komatsu, Hirohisa Izumi, Takashi Shimizu, Yasuhiro Takeda, Masashi Kuwahata
FRONTIERS IN NUTRITION
(2018)