Article
Chemistry, Analytical
Ji Hong Kim, Chae Hwan Cho, Jae Hwan Shin, Moon Seop Hyun, Eunha Hwang, Tae Jung Park, Jong Pil Park
Summary: An electrochemical biosensor was developed to detect the HA protein in the envelope of the influenza virus, using high-affinity peptide-displaying phage particles identified through phage display technology. The sensor showed good performance characteristics, including low limit of detection, high reproducibility, and compatibility with human plasma samples. The sensor system demonstrated advantages such as low cost, rapid analysis, and minimal sample volume requirement, making it suitable for rapid and effective detection of the influenza virus antigen.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Biochemistry & Molecular Biology
Mariangela Agamennone, Marialuigia Fantacuzzi, Giovanni Vivenzio, Maria Carmina Scala, Pietro Campiglia, Fabiana Superti, Marina Sala
Summary: Influenza viruses are a major cause of high morbidity and mortality worldwide. Current approaches for fighting flu include seasonal vaccines and antiviral drugs, but they have limitations. Due to the highly mutative nature of influenza viruses, there is an urgent need for the development of new antiviral therapies, and peptide-based therapies show great promise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Kin Kui Lai, James B. B. Munro, Guoli Shi, Saliha Majdoul, Alex A. A. Compton, Alan Rein
Summary: Serine incorporator 5 (Ser5) is a host antiviral factor against HIV-1, gammaretroviruses, and Influenza A virus (IAV). It inhibits virus-cell fusion by interacting with HA proteins and destabilizing the pre-fusion conformation of IAV HA. This study provides insights into the antiviral mechanism of Ser5.
Article
Multidisciplinary Sciences
Yura Shin, Jeonghun Kim, Jong Hyeon Seok, Heedo Park, Hye-Ran Cha, Si Hwan Ko, Jae Myun Lee, Man-Seong Park, Jung-Hwan Park
Summary: In this study, H3N2 microneedle vaccines were prepared using HPLC without the use of an antibody, and the cross-protection of the vaccines against multiple antigenic variants was observed. The HPLC method showed good diagnostic performance and agreement with ELISA.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Anna Parys, Elien Vandoorn, Jacqueline King, Annika Graaf, Anne Pohlmann, Martin Beer, Timm Harder, Kristien Van Reeth
Summary: A pig farmer in the Netherlands was infected with a Eurasian avian-like swine influenza A(H1N1) virus that was also found in the farmed pigs. Antigenic and genetic characterization of both viruses was conducted. Continued surveillance of swine influenza A viruses is necessary for assessing risks in humans and pigs.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Liju Raju, Sousa Javan Nikkhah, Matthias Vandichel, Eswaran Rajkumar
Summary: In this research, we designed and synthesized a series of novel bipyridine cored poly(benzyl-ether) dendrons containing tertiary butyl esters at their periphery. The molecular structures of the synthesized dendrons were characterized using NMR and mass spectrometry. We investigated the solvent dependent hydrodynamic size of the dendrons in DMSO and water using DLS experiments and analyzed the water contact angle of the dendrons. Molecular dynamics simulations were performed to understand the structure and solvation behavior of the dendrons at the atomistic level. The modeling results confirmed that DMSO is a better solvent than water for dendrons, resulting in a more unfolded molecular structure. Based on these results, these dendritic polymers are excellent candidates for multifunctional theranostics platforms.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
David F. Zeigler, Emily Gage, Christopher H. Clegg
Summary: The study introduced a nanoparticle-based vaccine platform for building broadly protective influenza vaccines targeting conserved epitopes, providing long-lasting immunity. Novel epitopes with higher protective potential were identified using protein sequence and structural information in combination, leading to improved antibody responses.
Article
Multidisciplinary Sciences
Kevin R. McCarthy, Tarra A. Von Holle, Laura L. Sutherland, Thomas H. Oguin, Gregory D. Sempowski, Stephen C. Harrison, M. Anthony Moody
Summary: Immune memory established by initial infection with influenza virus has a lasting imprint on later responses, which differ from those induced by early childhood immunization. A study comparing immune imprints from vaccination and infection in non-human primates found that initial exposure by infection led to strong but limited antibody responses, while initial vaccination elicited weaker but broader binding responses to different HA strains. This suggests that the mode of initial exposure affects the strength and breadth of the immune response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Brianna L. Bullard, Brigette N. Corder, Jennifer DeBeauchamp, Adam Rubrum, Bette Korber, Richard J. Webby, Eric A. Weaver
Summary: In this study, a universal swine H3 influenza vaccine was designed using a computational algorithm, which showed significant cross-reactive antibody and T-cell responses in both murine and swine models. The vaccine demonstrated superior cross-reactive immunity and reduced weight loss and lung viral titers in mice challenged with different swH3 viruses. The findings suggest that this epigraph vaccination strategy should be further investigated as a potential universal swH3 vaccine.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Karen Lizbeth Reyes-Barrera, Ruth Elena Soria-Guerra, Rogelio Lopez-Martinez, Leonor Huerta, Nohemi Salinas-Jazmin, Carlos Cabello-Gutierrez, Angel Gabriel Alpuche-Solis
Summary: The study demonstrates that expressing the EB peptide in microalgae could be a promising approach for antiviral therapy, showing significant efficacy in inhibiting the spread and replication of influenza viruses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Maria Auladell, Hoang Vu Mai Phuong, Le Thi Quynh Mai, Yeu-Yang Tseng, Louise Carolan, Sam Wilks, Pham Quang Thai, David Price, Nguyen Thanh Duong, Nguyen Le Khang Hang, Le Thi Thanh, Nguyen Thi Hong Thuong, Tran Thi Kieu Huong, Nguyen Thi Ngoc Diep, Vu Thi Ngoc Bich, Arseniy Khvorov, Luca Hensen, Tran Nhu Duong, Katherine Kedzierska, Dang Duc Anh, Heiman Wertheim, Scott D. Boyd, Kim L. Good-Jacobson, Derek Smith, Ian Barr, Sheena Sullivan, H. Rogier van Doorn, Annette Fox
Summary: Recent prior influenza A infection enhances antibody responses to subsequent influenza vaccination and broadens the reactivity to different strains. Immunological memory induced by prior infection plays an important role in vaccine responses.
Article
Microbiology
Ekaterina O. Sinegubova, Olga A. Kraevaya, Aleksandrina S. Volobueva, Alexander V. Zhilenkov, Alexander F. Shestakov, Sergey V. Baykov, Pavel A. Troshin, Vladimir V. Zarubaev
Summary: The influenza virus has a high mutation rate, resulting in rapid emergence of drug-resistant strains. Therefore, there is a need for the development of new broad-spectrum antivirals against influenza. This study describes derivatives based on fullerenes that have broad virus inhibiting activities against a panel of influenza viruses. Compound 2, containing residues of salts of 2-amino-3-cyclopropylpropanoic acid, exhibited the highest antiviral activity and lowest toxicity.
Article
Multidisciplinary Sciences
Corey Momont, Ha V. V. Dang, Fabrizia Zatta, Kevin Hauser, Caihong Wang, Julia di Iulio, Andrea Minola, Nadine Czudnochowski, Anna De Marco, Kaitlin Branch, David Donermeyer, Siddhant Vyas, Alex Chen, Elena Ferri, Barbara Guarino, Abigail E. Powell, Roberto Spreafico, Samantha S. Yim, Dale R. Balce, Istvan Bartha, Marcel Meury, Tristan I. Croll, David M. Belnap, Michael A. Schmid, William Timothy Schaiff, Jessica L. Miller, Elisabetta Cameroni, Amalio Telenti, Herbert W. Virgin, Laura E. Rosen, Lisa A. Purcell, Antonio Lanzavecchia, Gyorgy Snell, Davide Corti, Matteo Samuele Pizzuto
Summary: The study describes a monoclonal antibody, FNI9, targeting neuraminidase, which can effectively inhibit the enzymatic activity of all group 1 and group 2 influenza A viruses, as well as certain influenza B viruses. FNI9 also shows broad neutralizing activity against seasonal influenza viruses and can synergize with antibodies targeting the hemagglutinin stem. The potent prophylactic activity of FNI9 against lethal influenza A and B virus infections in mice supports its development for the prevention of influenza illness.
Article
Biochemistry & Molecular Biology
Elisa Franzi, Gregory Mathez, Soraya Dinant, Charlotte Deloizy, Laurent Kaiser, Caroline Tapparel, Ronan Le Goffic, Valeria Cagno
Summary: Influenza virus is a major cause of respiratory infections worldwide, and finding alternative antiviral strategies is necessary despite the availability of vaccines and antiviral drugs. This study identifies non-steroidal estrogens as potent inhibitors of influenza virus by interacting with the hemagglutinin protein to prevent viral entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Monika Mullerova, Michaela Hovorkova, Tana Zavodna, Lucie Cervenkova Stastna, Alena Krupkova, Vojtech Hamala, Katerina Novakova, Jan Topinka, Pavla Bojarova, Tomas Strasak
Summary: Galectins and their carbohydrate ligands play a vital role in regulating various biological processes. Carbosilane dendrimers show promise as a nanoscaffold for presenting multivalent carbohydrate ligands to target galectin receptors. This study presents a synthetic method for lactose-functionalized carbosilane glycodendrimers and demonstrates their high affinity to galectin-9. Multivalent presentation of the ligand significantly enhances its inhibitory effect, as shown by the 1400-fold higher potency of the third-generation dendritic ligand compared to monovalent lactose. The increased affinity is attributed to the formation of stable galectin/lactose-functionalized carbosilane glycodendrimer aggregates.
Article
Chemistry, Medicinal
Koji Matsuoka, Yuya Suzuki, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2020)
Article
Chemistry, Multidisciplinary
Teruhiko Matsubara, Kazutoshi IIjima, Takahiro Kojima, Miwa Hirai, Erika Miyamoto, Toshinori Sato
Summary: The study showed that a peptide can serve as a molecular probe to recognize GM1 nanodomains on the cell surface, specifically binding to high-density ganglioside nanodomains that resist MβCD treatment. This peptide probe is helpful for studying lipid organization on the cell membrane and understanding how the lateral distribution of gangliosides impacts biological functions and disease onset.
Article
Chemistry, Organic
Jianhong Zhang, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano, Koji Matsuoka
Summary: Efficient synthesis of lauryl thioglycoside of Neu5Gc was achieved from a known thioglycoside of Neu5Ac. The reactivity of Neu5Gc thioglycoside in O-glycosidation was investigated, showing good activities with simple alcohols. The thioglycoside also demonstrated potential as a glycosyl donor in reactions with glucose and lactose derivatives.
TETRAHEDRON LETTERS
(2021)
Article
Chemistry, Organic
Yoshihiro Ishimaru, Yosuke Saito, Yoko S. Shiraishi, Yasuaki Esumi, Daiyo Terunuma, Hiroyoshi Kuzuhara, Koji Matsuoka
Summary: The synthetic assembly of two beta-cyclodextrins using a 6,6'-di-iodotrehalose derivative as a linker was efficiently achieved in liquid ammonia through a one-pot reaction. The resulting CD dimer with a unique sulfide linkage displayed a wider hydrophobic region, and its inclusion property was tentatively examined using TNS as a model guest compound.
TETRAHEDRON LETTERS
(2021)
Article
Chemistry, Medicinal
Koji Matsuoka, Takayuki Kaneshima, Ryota Adachi, Jiei Sasaki, Takao Hashiguchi, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano
Summary: A water-soluble glycomonomer with SLac moiety was prepared using Schmidt's protocol, and three glycopolymers with different sugar densities were obtained through polymerization, with the one with low sugar density exhibiting the highest inhibitory potency.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Polymer Science
Koji Matsuoka, Masaki Nakagawa, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano
Summary: This study investigated the interaction between glycopolymers and proteins by varying the length and density of carbohydrate residues. The results showed that the glycopolymers with the longest methylene length and a specific ratio exhibited the highest interaction strength.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Polymer Science
Shogo Ishizaka, Shintaro Nakagawa, Koji Matsuoka, Naoko Yoshie
Summary: In this study, we synthesized multiphase copolymers with a gradual change in hydrogen bond density using a simple one-pot copolymerization method. The mechanical properties of the copolymers depended on the number of hydrogen bonding units, with more units leading to higher rigidity and toughness, and fewer units contributing to better fatigue recoverability. This research provides a new design principle for creating tough polymer materials.
Article
Chemistry, Organic
Koji Matsuoka, Daiki Endo, Ryota Adachi, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano
Summary: Verification of acetylation for a cyclic α-N-β-D-furanose was achieved and the corresponding mono alcohol was obtained. Structural elucidation of the derivatives was conducted based on NMR spectroscopy. Complete protection by esterification and methylation yielded fully protected compounds, with NMR spectroscopy confirming the success of the reaction.
TETRAHEDRON LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Takahiko Matsushita, Naomichi Toda, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: In this study, N-acetylglucosamine-terminated hexavalent carbosilane dendrimers were synthesized and their binding to wheat germ agglutinin (WGA) was investigated. The results showed that these glycodendrimers exhibited high affinity with WGA, with 1a and 1b showing the strongest affinity.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Takahiko Matsushita, Naoto Maruyama, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: This study evaluated the potencies of dibromopyridazinediones with mono- and double-biotin groups as cysteine-selective biotinylation reagents by conjugating them with a goat anti-mouse IgG Fab fragment. The results showed that these reagents can introduce biotin groups into the reduced Fab and re-bridge the disulfide moiety.
Article
Biochemistry & Molecular Biology
Ryota Adachi, Takahiko Matsushita, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: Glycopolymers with sialyl alpha 2-3 lactose moieties were prepared using longer spacer arms. Radical polymerization of glycomonomers produced glycopolymers with varying sugar densities. Fluorometric analyses using wheat germ agglutinin (WGA) showed unique binding specificities based on sugar densities.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Polymer Science
Ryota Adachi, Takahiko Matsushita, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: A polymerizable alcohol with 9 PEG repeats was prepared to imitate an oligosaccharide. Sialyl alpha(2 -> 3) lactose, the sugar moiety of GM3 ganglioside, was also prepared and condensed with the polymerizable alcohol to form the desired glycomonomer, which was further polymerized with or without acrylamide to yield water-soluble glycopolymers. The glycopolymers exhibited higher affinities compared to those with shorter aglycon moieties.
Article
Biochemistry & Molecular Biology
Takahiko Matsushita, Hinako Yamochi, Shinzo Omiya, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: Polyacrylamides incorporating serine, aspartic acid, and histidine, the amino acids in the catalytic triad of natural serine protease chymotrypsin, were synthesized and their protein cleavage activity was examined. SDS-PAGE analysis revealed that some of the synthesized ternary copolymers were able to cleave bovine and human serum albumins. Homopolymers incorporating a single type of amino acid also showed protein cleavage abilities. These findings suggest that polymers functionalized with amino acids have potential as proteolytic artificial enzymes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Takahiko Matsushita, Momoka Nozaki, Mio Sunaga, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: In this study, glycomonomers with N-glycosidic linkages were prepared using a known glycosyl amine, GlcNAc. Radical polymerization of the glycomonomers resulted in a series of glycopolymers with varying sugar densities, serving as models for the core structure of Asn-linked-type glycoproteins. Additionally, the fluorometric analysis demonstrated unique binding specificities of wheat germ agglutinin towards the glycopolymers based on the flexibility of sugar moieties.
Article
Chemistry, Multidisciplinary
Yoshihiro Ishimaru, Tomohide Moteki, Miho Suzuki, Tetsuo Koyama, Takahiko Matsushita, Ken Hatano, Koji Matsuoka
Summary: A water-soluble glycopolymer bearing porphyrin moieties was synthesized by radical polymerization, and its potential as an anti-cancer agent was confirmed.
